Next Article in Journal
Metformin Restores Parkin-Mediated Mitophagy, Suppressed by Cytosolic p53
Previous Article in Journal
Cloning and Transcriptional Activity of the Mouse Omi/HtrA2 Gene Promoter
Open AccessArticle

Platelet-Rich Plasma Increases the Levels of Catabolic Molecules and Cellular Dedifferentiation in the Meniscus of a Rabbit Model

Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea
Department of Orthopedic Surgery, College of Medicine, Yeungnam University, Daegu 705-717, Korea
Cardiovascular Product Evaluation Center, College of Medicine, Yonsei University, Seoul 120-752, Korea
School of Materials Science and Engineering, Yeungnam University, Gyeongsan 712-749, Korea
Author to whom correspondence should be addressed.
Academic Editor: Joseph V. Moxon
Int. J. Mol. Sci. 2016, 17(1), 120;
Received: 26 August 2015 / Revised: 24 November 2015 / Accepted: 11 January 2016 / Published: 16 January 2016
(This article belongs to the Section Biochemistry)
Despite the susceptibility to frequent intrinsic and extrinsic injuries, especially in the inner zone, the meniscus does not heal spontaneously owing to its poor vascularity. In this study, the effect of platelet-rich plasma (PRP), containing various growth factors, on meniscal mechanisms was examined under normal and post-traumatic inflammatory conditions. Isolated primary meniscal cells of New Zealand white (NZW) rabbits were incubated for 3, 10, 14 and 21 days with PRP(−), 10% PRP (PRP(+)), IL(+) or IL(+)PRP(+). The meniscal cells were collected and examined using reverse-transcription polymerase chain reaction (RT-PCR). Culture media were examined by immunoblot analyses for matrix metalloproteinases (MMP) catabolic molecules. PRP containing growth factors improved the cellular viability of meniscal cells in a concentration-dependent manner at Days 1, 4 and 7. However, based on RT-PCR, meniscal cells demonstrated dedifferentiation, along with an increase in type I collagen in the PRP(+) and in IL(+)PRP(+). In PRP(+), the aggrecan expression levels were lower than in the PRP(−) until Day 21. The protein levels of MMP-1 and MMP-3 were higher in each PRP group, i.e., PRP(+) and IL(+)PRP(+), at each culture time. A reproducible 2-mm circular defect on the meniscus of NZW rabbit was used to implant fibrin glue (control) or PRP in vivo. After eight weeks, the lesions in the control and PRP groups were occupied with fibrous tissue, but not with meniscal cells. This study shows that PRP treatment of the meniscus results in an increase of catabolic molecules, especially those related to IL-1α-induced inflammation, and that PRP treatment for an in vivo meniscus injury accelerates fibrosis, instead of meniscal cartilage. View Full-Text
Keywords: platelet-rich plasma; meniscal cells; MMPs; proteoglycan; collagen platelet-rich plasma; meniscal cells; MMPs; proteoglycan; collagen
Show Figures

Graphical abstract

MDPI and ACS Style

Lee, H.-R.; Shon, O.-J.; Park, S.-I.; Kim, H.-J.; Kim, S.; Ahn, M.-W.; Do, S.H. Platelet-Rich Plasma Increases the Levels of Catabolic Molecules and Cellular Dedifferentiation in the Meniscus of a Rabbit Model. Int. J. Mol. Sci. 2016, 17, 120.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop