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A Combined Metabolomic and Proteomic Analysis of Gestational Diabetes Mellitus

Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 6 Grunwaldzka Street, Poznań 60-780, Poland
Obstetrics and Gynecology Ward, District Hospital in Mielec, 22a Żeromskiego Street, Mielec 39-300, Poland
Departmentof Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Święcickiego 4 Street, Poznań 60-781, Poland
Institute of Human Genetics, Polish Academy of Sciences, 32 Strzeszyńska Street, Poznań 60-479, Poland
Gynecologic Oncology Department, Poznan University of Medical Sciences, Polna 33 Street, Poznań 60-535, Poland
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: William Chi-shing Cho
Int. J. Mol. Sci. 2015, 16(12), 30034-30045;
Received: 3 July 2015 / Revised: 28 October 2015 / Accepted: 20 November 2015 / Published: 16 December 2015
(This article belongs to the Collection Advances in Proteomic Research)
PDF [1469 KB, uploaded 16 December 2015]


The aim of this pilot study was to apply a novel combined metabolomic and proteomic approach in analysis of gestational diabetes mellitus. The investigation was performed with plasma samples derived from pregnant women with diagnosed gestational diabetes mellitus (n = 18) and a matched control group (n = 13). The mass spectrometry-based analyses allowed to determine 42 free amino acids and low molecular-weight peptide profiles. Different expressions of several peptides and altered amino acid profiles were observed in the analyzed groups. The combination of proteomic and metabolomic data allowed obtaining the model with a high discriminatory power, where amino acids ethanolamine, l-citrulline, l-asparagine, and peptide ions with m/z 1488.59; 4111.89 and 2913.15 had the highest contribution to the model. The sensitivity (94.44%) and specificity (84.62%), as well as the total group membership classification value (90.32%) calculated from the post hoc classification matrix of a joint model were the highest when compared with a single analysis of either amino acid levels or peptide ion intensities. The obtained results indicated a high potential of integration of proteomic and metabolomics analysis regardless the sample size. This promising approach together with clinical evaluation of the subjects can also be used in the study of other diseases. View Full-Text
Keywords: gestational diabetes mellitus; mass spectrometry; combined metabolomic and proteomic approach; chemometric analysis gestational diabetes mellitus; mass spectrometry; combined metabolomic and proteomic approach; chemometric analysis

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Hajduk, J.; Klupczynska, A.; Dereziński, P.; Matysiak, J.; Kokot, P.; Nowak, D.M.; Gajęcka, M.; Nowak-Markwitz, E.; Kokot, Z.J. A Combined Metabolomic and Proteomic Analysis of Gestational Diabetes Mellitus. Int. J. Mol. Sci. 2015, 16, 30034-30045.

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