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Open AccessArticle

Transcriptome Profiling of Louisiana iris Root and Identification of Genes Involved in Lead-Stress Response

by Songqing Tian 1,2,†, Chunsun Gu 1,†, Liangqin Liu 3,†, Xudong Zhu 1,2, Yanhai Zhao 1 and Suzhen Huang 1,*
1
Institute of Botany, Jiangsu Province and Chinese Academy of Science, Nanjing 210014, China
2
Suzhou Polytechnical Institute of Agriculture, Suzhou 215008, China
3
College of Horticulture, Nanjing Agricultural University, Nanjing 210014, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Eleftherios P. Eleftheriou
Int. J. Mol. Sci. 2015, 16(12), 28087-28097; https://doi.org/10.3390/ijms161226084
Received: 6 June 2015 / Revised: 27 October 2015 / Accepted: 11 November 2015 / Published: 25 November 2015
Louisiana iris is tolerant to and accumulates the heavy metal lead (Pb). However, there is limited knowledge of the molecular mechanisms behind this feature. We describe the transcriptome of Louisiana iris using Illumina sequencing technology. The root transcriptome of Louisiana iris under control and Pb-stress conditions was sequenced. Overall, 525,498 transcripts representing 313,958 unigenes were assembled using the clean raw reads. Among them, 43,015 unigenes were annotated and their functions classified using the euKaryotic Orthologous Groups (KOG) database. They were divided into 25 molecular families. In the Gene Ontology (GO) database, 50,174 unigenes were categorized into three GO trees (molecular function, cellular component and biological process). After analysis of differentially expressed genes, some Pb-stress-related genes were selected, including biosynthesis genes of chelating compounds, metal transporters, transcription factors and antioxidant-related genes. This study not only lays a foundation for further studies on differential genes under Pb stress, but also facilitates the molecular breeding of Louisiana iris. View Full-Text
Keywords: Iris; Lead; transcriptome; RT-qPCR Iris; Lead; transcriptome; RT-qPCR
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Tian, S.; Gu, C.; Liu, L.; Zhu, X.; Zhao, Y.; Huang, S. Transcriptome Profiling of Louisiana iris Root and Identification of Genes Involved in Lead-Stress Response. Int. J. Mol. Sci. 2015, 16, 28087-28097.

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