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Int. J. Mol. Sci. 2015, 16(10), 24403-24416;

Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice

College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China
Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan 430023, China
Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangzhou 510610, China
Author to whom correspondence should be addressed.
Academic Editor: Chang Won Choi
Received: 4 August 2015 / Revised: 21 September 2015 / Accepted: 8 October 2015 / Published: 15 October 2015
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [806 KB, uploaded 15 October 2015]   |  


A high performance size exclusion-fluorescence detection (HPSEC-FD) method combined with fluorescein isothiocyanate (FITC) prelabeling was established for the microanalysis of polysaccharide–protein complexes from longan pulp (LPP). FITC-labeled LPP (LPPF) was fractionated by gel filtration chromatography. The weight-average molecular weight and FITC substitution degree of LPPF were 39.01 kDa and 0.20%, respectively. The HPSEC-FD calibration curves linear over the range of 1–200 µg/mL in mouse plasma, spleen and lung samples with correlation coefficients greater than 0.995. The inter-day and intra-day precisions of the method were not more than 6.9%, and the relative recovery ranged from 93.7% to 106.4%. The concentration–time curve of LPPF in plasma following intravenous (i.v.) administration at 40 mg/kg body weight well fitted to a two-compartment model. LPPF rapidly eliminated from plasma according to the short half-lives (t1/2α = 2.23 min, t1/2β = 39.11 min) and mean retention times (MRT0–t = 1.15 h, MRT0–∞ = 1.39 h). After administration over 5 to 360 min, the concentration of LPPF in spleen homogenate decreased from 7.41 to 3.68 µg/mL; the concentration in lung homogenate decreased from 9.08 to 3.40 µg/mL. On the other hand, the increasing concentration of LPPF fraction with low molecular weight in heart homogenate was observed. View Full-Text
Keywords: longan pulp; polysaccharide-protein complex; HPSEC-FD; pharmacokinetics; distribution longan pulp; polysaccharide-protein complex; HPSEC-FD; pharmacokinetics; distribution

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Min, T.; Sun, J.; Yi, Y.; Wang, H.-X.; Hang, F.; Ai, Y.-W.; Wang, L.-M. Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice. Int. J. Mol. Sci. 2015, 16, 24403-24416.

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