Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purification of Longan Polysaccharide Complexes (LPP) and Fluorescein Isothiocyanate-Labeled LPP (LPPF)
2.2. Spectral Features of LPP and LPPF
2.3. Method Validation
Biological Samples | Standard Curves | Correlation Coefficients | Linear Ranges * (µg/mL) |
---|---|---|---|
Blank | y = 2.199x + 2.273 | 0.998 | 1–200 µg/mL |
Plasma | y = 2.223x + 1.034 | 0.996 | 1–200 µg/mL |
Spleen | y = 2.349x − 0.485 | 0.996 | 1–200 µg/mL |
Lung | y = 2.138x + 2.616 | 0.995 | 1–200 µg/mL |
Biological Samples | Added Concentration (µg/mL) | Relative Recovery (%) | Precision (RSD, %) | |
---|---|---|---|---|
Intra-Day | Inter-Day | |||
Blank | 2 | 99.4 ± 4.3 | 3.4 | 4.0 |
40 | 101.7 ± 3.0 | 2.4 | 2.9 | |
80 | 101.4 ± 2.3 | 3.1 | 4.2 | |
Plasma | 2 | 98.2 ± 5.1 | 4.5 | 4.5 |
40 | 102.3 ± 4.1 | 3.2 | 2.9 | |
80 | 103.4 ± 5.2 | 5.1 | 2.6 | |
Spleen | 2 | 106.4 ± 3.9 | 3.6 | 3.5 |
40 | 96.7 ± 5.7 | 4.4 | 5.0 | |
80 | 105.4 ± 3.3 | 5.7 | 3.5 | |
Lung | 2 | 93.7 ± 2.9 | 3.0 | 4.2 |
40 | 104.2 ± 3.1 | 4.4 | 3.8 | |
80 | 96.5 ± 3.4 | 2.5 | 6.9 |
2.4. Pharmacokinetics and Tissue Distribution of LPPF
Parameters * | Values |
---|---|
Half-life of distribution phase t1/2α (min) | 2.23 ± 0.35 |
Half-life of elimination phase t1/2β (min) | 39.11 ± 2.73 |
Apparent volume of distribution of the central compartment V1 (L/kg) | 0.09 ± 0.01 |
Apparent volume of distribution of the peripheral compartment V2 (L/kg) | 0.26 ± 0.03 |
Clearance of the central compartment CL1 (L/h/kg) | 0.53 ± 0.03 |
Clearance of the peripheral compartment CL2 (L/h/kg) | 0.60 ± 0.05 |
Area under concentration–time curve AUC0–t (mg·h/L) | 56.80 ± 3.44 |
Area under concentration–time curve AUC0–∞ (mg·h/L) | 58.85 ± 3.51 |
Mean retention time MRT0–t (h) | 1.15 ± 0.04 |
Mean retention time MRT0–∞ (h) | 1.39 ± 0.08 |
3. Experimental Section
3.1. Materials
3.2. Animals and Biological Sample Preparation
3.3. Polysaccharide–Protein Complex Preparation
3.4. Spectrum Analysis
3.5. HPSEC Analysis
3.5.1. Detection Conditions
3.5.2. Selectivity
3.5.3. Quantitative Determination
3.5.4. Precision and Accuracy
3.6. Animal Experiment
3.7. Data Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Min, T.; Sun, J.; Yi, Y.; Wang, H.-X.; Hang, F.; Ai, Y.-W.; Wang, L.-M. Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice. Int. J. Mol. Sci. 2015, 16, 24403-24416. https://doi.org/10.3390/ijms161024403
Min T, Sun J, Yi Y, Wang H-X, Hang F, Ai Y-W, Wang L-M. Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice. International Journal of Molecular Sciences. 2015; 16(10):24403-24416. https://doi.org/10.3390/ijms161024403
Chicago/Turabian StyleMin, Ting, Jie Sun, Yang Yi, Hong-Xun Wang, Fei Hang, You-Wei Ai, and Li-Mei Wang. 2015. "Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice" International Journal of Molecular Sciences 16, no. 10: 24403-24416. https://doi.org/10.3390/ijms161024403