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Int. J. Mol. Sci. 2014, 15(10), 17806-17826;

Atrazine Affects Phosphoprotein and Protein Expression in MCF-10A Human Breast Epithelial Cells

Department of Agriculture & Environmental Science, Lincoln University of Missouri, Jefferson City, MO 65120, USA
Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA
Authors to whom correspondence should be addressed.
Received: 23 May 2014 / Revised: 26 August 2014 / Accepted: 11 September 2014 / Published: 1 October 2014
(This article belongs to the Collection Advances in Proteomic Research)
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Atrazine, a member of the 2-chloro-s-triazine family of herbicides, is the most widely used pesticide in the world and often detected in agriculture watersheds. Although it was generally considered as an endocrine disruptor, posing a potential threat to human health, the molecular mechanisms of atrazine effects remain unclear. Using two-dimensional gel electrophoresis, we identified a panel of differentially expressed phosphoproteins and total proteins in human breast epithelial MCF-10A cells after being exposed to environmentally relevant concentrations of atrazine. Atrazine treatments for 6 h resulted in differential expression of 4 phosphoproteins and 8 total-proteins as compared to the control cells (>1.5-fold, p < 0.05). MALDI-TOF MS/MS analysis revealed that the differentially expressed proteins belong to various cellular compartments (nucleus, cytosol, membrane) and varied in function, including those regulating the stress response such as peroxiredoxin I, HSP70 and HSP27; structural proteins such as tropomyosin and profilin 1; and oncogenesis proteins such as ANP32A. Six of the 12 identified proteins were verified by quantitative PCR for their transcript levels. The most up-regulated phosphoprotein by atrazine treatment, ANP32A, was further analyzed for its expression, distribution and cellular localization using Western blot and immunocytochemical approaches. The results revealed that ANP32 expression after atrazine treatment increased dose and time dependently and was primarily located in the nucleus. This study may provide new evidence on the potential toxicity of atrazine in human cells. View Full-Text
Keywords: Atrazine; MCF-10A cells; 2DE; protein expression; ANP32A Atrazine; MCF-10A cells; 2DE; protein expression; ANP32A

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Huang, P.; Yang, J.; Song, Q. Atrazine Affects Phosphoprotein and Protein Expression in MCF-10A Human Breast Epithelial Cells. Int. J. Mol. Sci. 2014, 15, 17806-17826.

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