Next Article in Journal
Regulation of Huntingtin Gene Expression by miRNA-137, -214, -148a, and Their Respective isomiRs
Next Article in Special Issue
The Key Enzyme of the Sialic Acid Metabolism Is Involved in Embryoid Body Formation and Expression of Marker Genes of Germ Layer Formation
Previous Article in Journal
Structural Properties of Polyphenols Causing Cell Cycle Arrest at G1 Phase in HCT116 Human Colorectal Cancer Cell Lines
Open AccessArticle

Decreased Expression of Alpha-L-Fucosidase Gene FUCA1 in Human Colorectal Tumors

Department of Biochemistry, Genetics and Immunology, University of Vigo, Campus As Lagoas Marcosende s/n, 36310 Vigo, Spain
Unit of Molecular Oncology, Department of Basic Research, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT), 28040 Madrid, Spain
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(8), 16986-16998;
Received: 2 July 2013 / Revised: 7 August 2013 / Accepted: 8 August 2013 / Published: 19 August 2013
(This article belongs to the Special Issue Glycosylation and Glycoproteins)
In previous studies we described a decreased alpha-L-fucosidase activity in colorectal tumors, appearing as a prognostic factor of tumoral recurrence. The aim of this work was to extend the knowledge about tissue alpha-L-fucosidase in colorectal cancer by quantifying the expression of its encoding gene FUCA1 in tumors and healthy mucosa. FUCA1 mRNA levels were measured by RT-qPCR in paired tumor and normal mucosa tissues from 31 patients. For the accuracy of the RT-qPCR results, five candidate reference genes were validated in those samples. In addition, activity and expression of alpha-L-fucosidase in selected matched tumor and healthy mucosa samples were analyzed. According to geNorm and NormFinder algorithms, RPLP0 and HPRT1 were the best reference genes in colorectal tissues. These genes were used for normalization of FUCA1 expression levels. A significant decrease of more than 60% in normalized FUCA1 expression was detected in tumors compared to normal mucosa (p = 0.002). Moreover, a gradual decrease in FUCA1 expression was observed with progression of disease from earlier to advanced stages. These findings were confirmed by Western blot analysis of alpha-L-fucosidase expression. Our results demonstrated diminished FUCA1 mRNA levels in tumors, suggesting that expression of tissue alpha-L-fucosidase could be regulated at transcriptional level in colorectal cancer. View Full-Text
Keywords: alpha-L-fucosidase; colorectal cancer; FUCA1; RT-qPCR alpha-L-fucosidase; colorectal cancer; FUCA1; RT-qPCR
Show Figures

Graphical abstract

MDPI and ACS Style

Otero-Estévez, O.; Martínez-Fernández, M.; Vázquez-Iglesias, L.; Páez de la Cadena, M.; Rodríguez-Berrocal, F.J.; Martínez-Zorzano, V.S. Decreased Expression of Alpha-L-Fucosidase Gene FUCA1 in Human Colorectal Tumors. Int. J. Mol. Sci. 2013, 14, 16986-16998.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Search more from Scilit
Back to TopTop