The Enzyme-Mediated Direct Reversal of a Dithymine Photoproduct in Germinating Endospores
Abstract
:1. The Unique Photo-Damage in Bacterial Endospores
2. SP Repair by NER and SPL
3. Mechanism of SPL Repair
4. The Stereoselectivity of SPL Reaction
5. SPL Catalysis Requires an Essential Cysteine
6. The Presence of a Novel Radical Transfer Pathway Mediated by Tyrosine Residues in SPL
7. Summary and Outlook
Acknowledgements
Conflict of Interest
References
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SPL enzyme | v (min−1) | Apparent KIE | Competitive KIE | SP repaired/5′-dA |
---|---|---|---|---|
W.T. | 0.41 ± 0.03 | 2.8 ± 0.3 | 3.4 ± 0.3 | 1.5 ± 0.2 |
C141A(Bs) | 0.14 ± 0.02 | 1.7 ± 0.2 | 3.0 ± 0.3 | 1.08 ± 0.1 |
Y97,99A(Bs) | N.A. | N.A. | N.A. | N.A. |
Y97F(Bs) | 0.12 ± 0.01 | 16 ± 1.5 | 11.5 ± 1.5 | 1.6 ± 0.2 |
Y99F(Bs) | 0.06 ± 0.005 | 10.5 ± 1 | 9 ± 1 | 1.0 ± 0.1 |
Y97,99F(Bs) | <0.004 | N.A. | N.A. | 0.92 ± 0.1 |
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Yang, L.; Li, L. The Enzyme-Mediated Direct Reversal of a Dithymine Photoproduct in Germinating Endospores. Int. J. Mol. Sci. 2013, 14, 13137-13153. https://doi.org/10.3390/ijms140713137
Yang L, Li L. The Enzyme-Mediated Direct Reversal of a Dithymine Photoproduct in Germinating Endospores. International Journal of Molecular Sciences. 2013; 14(7):13137-13153. https://doi.org/10.3390/ijms140713137
Chicago/Turabian StyleYang, Linlin, and Lei Li. 2013. "The Enzyme-Mediated Direct Reversal of a Dithymine Photoproduct in Germinating Endospores" International Journal of Molecular Sciences 14, no. 7: 13137-13153. https://doi.org/10.3390/ijms140713137