Molecular System Bioenergics of the Heart: Experimental Studies of Metabolic Compartmentation and Energy Fluxes versus Computer Modeling †
Abstract
:1. Introduction
1.1. Some Historical Notes on the Metabolic Compartmentation of Adenine Nucleotides in Muscle Cells
1.2. Local Restrictions of ATP and ADP Diffusion, Their Binding to the Intracellular Structures and on the State of the Creatine Kinase Reaction in Muscle Cells
1.3. Direct Measurement of the Energy Fluxes in Vivo: The 18O Transfer Method
1.4. Problems of Computer Simulation of Muscle Energetic: Success and Failures
1.4.1. Original and Modified Models of Compartmentalized Energy Transfer
1.4.2. Multiscale “Sloppy” Modeling of CK Fluxes by Hetting—van Beek
1.4.3. PCr Fluxes Lost: The Vendelin-Hoerter’s Model
2. Conclusions
Acknowledgments
- †This work is dedicated to the memory of Professor Xavier Leverve. Professor Xavier Leverve (born in 1950) created the Laboratory of Fundamental and Applied Bioenergetics at the Joseph Fourier University in Grenoble, France, in 1995. He was one of the leading scientists in the field of bioenergetics, metabolism and nutrition. His interests were covering cellular bioenergetics and substrate metabolism, as well as hypoxia/reoxygenation and acid/base balance. Under his leadership, the Laboratory of Fundamental and Applied Bioenergetics of Joseph Fourier University became one of the most productive and influential in France, acknowledged for its high level of research by acceptance into Institute National de la Santé et la Recherche Medicale (INSERM) in 2002. He directed this laboratory very effectively and skillfully until the end of his days on November 8, 2010. He will always be missed.
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[ATP], mM | MtCK Rate (PCr Production), % of Maximum | ANT Rate (ATP Export), % of Maximum | PCr/O2 |
---|---|---|---|
1 | 62.61 | 53.55 | 7.01 |
2 | 63.95 | 52.12 | 7.36 |
5 | 64.83 | 46.19 | 8.44 |
10 | 65.17 | 38.22 | 10.2 |
System Description | tmito, s | Myoplasmic PCr/Cr in Diastole | Energy Export by PCr, % | ||
---|---|---|---|---|---|
Before Transition | After Transition | Before Transition | After Transition | ||
System complete with MtCK tightly coupled to ANT and severe restrictions for ATP/ADP diffusion on MOM | 3.9 | 2.64 | 1.82 | 91.4 | 90.0 |
System A with low MtCK activity with no coupling, but maximal restrictions on MOM | 8.7 | 1.82 | 0.88 | 73.2 | 62.7 |
System with no coupling and weak restrictions on MOM | 4.4 | 2.53 | 1.68 | 23.5 | 19.4 |
System with no coupling, weak restrictions on MOM and reduced metabolite contents | 2.9 | 1.96 | 1.28 | 23.3 | 19.4 |
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Aliev, M.; Guzun, R.; Karu-Varikmaa, M.; Kaambre, T.; Wallimann, T.; Saks, V. Molecular System Bioenergics of the Heart: Experimental Studies of Metabolic Compartmentation and Energy Fluxes versus Computer Modeling. Int. J. Mol. Sci. 2011, 12, 9296-9331. https://doi.org/10.3390/ijms12129296
Aliev M, Guzun R, Karu-Varikmaa M, Kaambre T, Wallimann T, Saks V. Molecular System Bioenergics of the Heart: Experimental Studies of Metabolic Compartmentation and Energy Fluxes versus Computer Modeling. International Journal of Molecular Sciences. 2011; 12(12):9296-9331. https://doi.org/10.3390/ijms12129296
Chicago/Turabian StyleAliev, Mayis, Rita Guzun, Minna Karu-Varikmaa, Tuuli Kaambre, Theo Wallimann, and Valdur Saks. 2011. "Molecular System Bioenergics of the Heart: Experimental Studies of Metabolic Compartmentation and Energy Fluxes versus Computer Modeling" International Journal of Molecular Sciences 12, no. 12: 9296-9331. https://doi.org/10.3390/ijms12129296