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Int. J. Mol. Sci. 2011, 12(11), 8275-8287;

Active Protein Aggregates Produced in Escherichia coli

Laboratory for Biosynthesis and Biotransformation, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
Medical Centre for Molecular Biology, Medical faculty, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia
Author to whom correspondence should be addressed.
Received: 12 October 2011 / Revised: 11 November 2011 / Accepted: 11 November 2011 / Published: 22 November 2011
(This article belongs to the Special Issue Protein Aggregation)
Full-Text   |   PDF [557 KB, uploaded 19 June 2014]


Since recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host organisms for economical protein production. However, upon over-expression, protein aggregates called inclusion bodies (IBs) are often formed. Until recently IBs formation represented a bottleneck in protein production as they were considered as deposits of inactive proteins. However, recent studies show that by choosing the appropriate host strain and designing an optimal production process, IBs composed from properly folded and biologically active recombinant proteins can be prepared. Such active protein particles can be further used for the isolation of pure proteins or as whole active protein particles in various biomedical and other applications. Therefore interest in understanding the mechanisms of their formation as well as their properties is increasing. View Full-Text
Keywords: E. coli; active protein aggregates; inclusion bodies; IBs E. coli; active protein aggregates; inclusion bodies; IBs
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Peternel, Š.; Komel, R. Active Protein Aggregates Produced in Escherichia coli. Int. J. Mol. Sci. 2011, 12, 8275-8287.

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