Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental
3.1. General
4. Conclusions
Acknowledgments
References
- Sohal, RS; Mockett, RJ; Orr, WC; Holbrook, NJ. Mechanisms of aging: An appraisal of the oxidative stress hypothesis. Free Rad. Biol. Med 2002, 33, 575–586. [Google Scholar]
- Hamilton, ML; Remmen, HV; Drake, JA; Yang, H; Guo, ZM; Kewitt, K; Walter, CA; Richardson, A. Does oxidative damage to DNA increase with age? Proc. Nat. Acad. Sci. USA 2001, 98, 10469–10474. [Google Scholar]
- Young, IS; Woodside, JV. Antioxidants in health and disease. J. Clin. Pathol 2001, 54, 176–186. [Google Scholar]
- Basile, A; Ferrara, L; Del Pozzo, M; Mele, G; Sorbo, S; Bassi, P; Montesano, D. Antibacterial and antioxidant activities of ethanol extract from Paullinia cupana Mart. J. Ethnopharmacol 2005, 102, 32–36. [Google Scholar]
- Yoo, HH; Park, JH; Kwon, SW. In vitro cytotoxic activity of some korean medicinal plants on human cancer cell lines: Enhancement in cytotoxicity by heat processing. Phytother. Res 2007, 21, 900–903. [Google Scholar]
- Coelho, MG; Cheddier, LM; Scio, E; Pimenta, DS. Alterações morfoanatômicas e químicas em Centella erecta (Linn. F.) Fernand., relacionadas à luminosidade e sazonalidade. In Proceedings of Livro de resumos XI Seminário Mineiro de Plantas Medicinais e IIa Jornada de Farmácia de Diamantina, Diamantina, Brazil; 2005; pp. 81–82. [Google Scholar]
- Handa, SS; Deepak, M; Mangal, AK. Centella asiatica. In Indian Herbal Pharmacopoeia, Indian Drug Manufacture; Mumbai and Regional Res. Lab.: Jammu-Tawi, India, 1988; pp. 47–55. [Google Scholar]
- Veerendrakumar, MH; Gupta, YK. Effect of different extracts of Centella asiatica on cognition and markers of oxidative stress in rats. J. Ethnopharmacol 2002, 79, 253–260. [Google Scholar]
- Matsuda, H; Morikawa, T; Ueda, H; Yoshikawa, M. Medicinal foodstuffs. XXVII. Saponin constituents Gotu Kola (2): Structures of new ursane- and oleanane-type triterpene oligoglycosides, centellasaponin B, C, and D, from Centella asiatica cultivated in Sri Lanka. Chem. Pharm. Bull 2001, 49, 1368–1371. [Google Scholar]
- Inamdar, PK; Yeole, RD; Ghogare, AB; de Souza, NJ. Determination of biologically active constituents in Centella asiatica. J. Chromatogr. A 1996, 742, 127–130. [Google Scholar]
- Bonfill, M; Mangas, S; Cusidó, RM; Osuna, L; Piñol, MT; Palazón, J. Identification of triterpenoid compounds of Centella asiatica by thin-layer chromatography and mass spectrometry. Biom. Chromatogr 2006, 20, 151–153. [Google Scholar]
- Coldren, CD; Hashim, P; Ali, JM; Oh, SK; Sinskey, AJ; Rha, C. Gene expression changes in the human fibroblast induced by Centella asiatica triterpenoids. Planta Med 2003, 69, 725–732. [Google Scholar]
- Babu, TD; Kuttan, G; Padikkala, J. Cytotoxic and antitumour properties of certain taxa of Umbelliferae with special reference to Centella asiatica (L.) Urban. J. Ethnopharmacol 1995, 48, 53–57. [Google Scholar]
- Gurfinkel, DM; Chow, S; Hurren, R; Gronda, M; Henderson, C; Berube, C; Hedley, DW; Schimmer, AD. Disruption of the endoplasmic reticulum and increases in cytoplasmic calcium are early events in cell death induced by the natural triterpenoid Asiatic acid. Apoptosis 2006, 11, 1463–1471. [Google Scholar]
- Park, BC; Bosire, KO; Lee, ES; Lee, YS; Kim, JA. Asiatic acid induces apoptosis in SK-MEL-2 human melanoma cells. Cancer Lett 2005, 218, 81–90. [Google Scholar]
- Cho, CW; Choi, DS; Cardone, MH; Kim, CW; Sinskey, AJ; Rha, C. Glioblastoma cell death induced by asiatic acid. Cell Bio. Toxicol 2006, 22, 393–408. [Google Scholar]
- Hsu, YL; Kuo, PL; Lin, LT; Lin, CC. Asiatic acid, a triterpene, induces apoptosis and cell cycle arrest through activation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways in human breast cancer cells. J. Pharmacol. Experim. Therap 2005, 313, 333–344. [Google Scholar]
- Park, BC; Paek, SH; Lee, YS; Kim, SJ; Lee, ES; Choi, HG; Yong, CS; Kim, JA. Inhibitory effects of asiatic acid on 7,12-dimethylbenz[α]anthracene and 12-O-tetradecanoylphorbol 13-acetate-induced tumor promotion in mice. Biol. Pharm. Bull 2007, 30, 176–179. [Google Scholar]
- Xu, BJ; Chang, SKC. A comparative study on phenolic profiles and antioxidant activities of Legumes as affected by extraction solvents. J. Food. Sci 2007, 72, 159–166. [Google Scholar]
- Zainol, MK; Abd-Hamid, A; Yusof, S; Muse, R. Antioxitative activity and total phenolic compounds of leaf, root, and petiole of four accessions of Centella asiatica (L.) Urban. Food Chem 2003, 81, 575–581. [Google Scholar]
- Robbers, JE; Speedie, MK; Tyler, VE. Pharmacognosy and Pharmacobiotecnology; Editora International Williams & Wilkins: Baltimore, USA, 1996; p. 128. [Google Scholar]
- Zuanazzi, JAS. Flavonóides. In Farmacognosia: da planta ao medicamento; Simões, CMO, Schenkel, EP, Gosmann, G, Mello, JCP, Mentz, LA, Petrovick, PR, Eds.; Editora UFSC: Florianópolis, Brazil, 2000; pp. 576–614. [Google Scholar]
- Mensor, LL; Menezes, FS; Leitão, GG; Reis, AS; Santos, TC; Coube, CS. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother. Res 2001, 15, 27–30. [Google Scholar]
- Cao, G; Sofic, E; Prior, RL. Antioxidant and prooxidant behavior of flavonoids: Structure-activity relationships. Free Rad. Biol. Med 1997, 22, 749–760. [Google Scholar]
- Madsen, HL; Nielsen, BR; Bertelsen, G; Skibsted, LH. Screening of antioxidative activity of spices. A comparison between assays based on ESR spins trapping and electrochemical measurement of oxygen consumption. Food Chem 1996, 57, 331–337. [Google Scholar]
- Moller, JKS; Madsen, HL; Altonen, T; Skibsted, LH. Dittany (Origanum dictamnus) as a source of water-extractable antioxidants. Food Chem 1999, 64, 215–219. [Google Scholar]
- Subathra, M; Shila, S; Devi, MA; Panneerselvam, C. Emerging role of Centella asiatica in improving age-related neurological antioxidant status. Exp. Geronto 2005, 40, 707–715. [Google Scholar]
- Kamuhabwa, A; Nshimo, C; de Witte, P. Cytotoxicity of some medicinal plant extracts used in Tanzanian traditional medicine. J. Ethnopharmacol 2000, 70, 143–149. [Google Scholar]
- Fiore, G; Nencini, C; Cavallo, F; Capasso, A; Bader, A; Giorgi, G; Micheli, L. In vitro antiproliferative effect of six salvia species on human tumor cell lines. Phytother. Res 2006, 20, 701–703. [Google Scholar]
- Stewart, DJ. Mechanisms of resistance to cisplatin and carboplatin. Crit. Rev. Oncol. Hematol 2007, 63, 12–31. [Google Scholar]
- Barbosa, NR; Pittella, F; Gattaz, WF. Centella asiatica water extract inhibits iPLA2 and cPLA2 activities in rat cerebellum. Phytomedicine 2008, 15, 896–900. [Google Scholar]
- Association of official analytical chemists. In Official Methods of Analysis of the Association of Official Analytical Chemists, 9th ed; Board: Washington, USA, 1960; p. 275.
- Vennat, B; Gross, D; Pourrat, H. Hamamelis virginiana: Identification and assay of proanthrocyanidins, phenolic acids and flavonoids in leaf extracts. Pharm. Acta Helv 1992, 67, 11–14. [Google Scholar]
- Sreejayan, N; Rao, MNA. Free radical scavenging activity of curcuminoids. Drug Res 1997, 46, 169–171. [Google Scholar]
- Denizot, F; Lang, R. Rapid colorimetric assay for cell growth and survival–modification to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immun. Meth 1986, 89, 271–277. [Google Scholar]
- Wallace, HJ; Higby, DJ. Recents Results in Cancer Research: Platinum Coordination Complexes in Cancer Chemotherapy; Connors, TA, Roberts, JJ, Eds.; Springer-Verlag: New York, NY, USA, 1974; p. 167. [Google Scholar]
- Wiltshaw, E; Roberts, JJ; Carr, B. Recents Results in Cancer Research Platinum Coordination Complexes in Cancer Chemotherapy; Springer-Verlag: New York, NY, USA, 1974; p. 178. [Google Scholar]
Cell lines | IC50 (μg/mL) | Taxol® (μg/mL) | |
---|---|---|---|
Tumoral | B16F1 | 698.0 | 3.55 × 10−6 |
MDA-MB 231 | 648.0 | 6.71 × 10−6 | |
C6 | 1000.0 | 3.43 × 10−6 | |
A549 | > 1000.0 | 6.03 × 10−6 | |
Normal | BHK-21 | > 1000.0 | 1.06 × 102 |
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Share and Cite
Pittella, F.; Dutra, R.C.; Junior, D.D.; Lopes, M.T.P.; Barbosa, N.R. Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb. Int. J. Mol. Sci. 2009, 10, 3713-3721. https://doi.org/10.3390/ijms10093713
Pittella F, Dutra RC, Junior DD, Lopes MTP, Barbosa NR. Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb. International Journal of Molecular Sciences. 2009; 10(9):3713-3721. https://doi.org/10.3390/ijms10093713
Chicago/Turabian StylePittella, Frederico, Rafael C. Dutra, Dalton D. Junior, Miriam T. P. Lopes, and Nádia R. Barbosa. 2009. "Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb." International Journal of Molecular Sciences 10, no. 9: 3713-3721. https://doi.org/10.3390/ijms10093713