Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration
Abstract
:1. Introduction
2. Results
2.1. Scanning Electron Microscopy (SEM)
2.2. Antibacterial Activity
2.3. Cell Viability
2.4. Alizarin Red S Staining
2.5. Alkaline Phosphatase (ALP) Activity
2.6. Quantitative Real-Time Polymerase Chain Reaction
3. Discussion
4. Materials and Methods
4.1. Scaffold Preparation
4.2. Peptide Loading
4.3. Scanning Electron Microscopy (SEM)
4.4. Antibacterial Test
4.5. Cell Seeding
4.6. Cytotoxicity Assay
4.7. Alizarin Red S Staining
4.8. Alkaline Phosphatase (ALP) Activity
4.9. Quantitative Real-Time Polymerase Chain Reaction
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Scaffold ID | CS (g) | HAp (g) | FAp (g) | Volume of Acetic Acid/(mL) |
---|---|---|---|---|
12.5 HAp | 1.5 | 1.5 | 0 | 12.5 |
20 HAp | 1.5 | 1.5 | 0 | 20 |
20 HAp/FAp | 1.5 | 0.75 | 0.75 | 20 |
30 HAp/FAp | 1.5 | 0.75 | 0.75 | 30 |
Gene | Primer Sequences Forward/Reverse |
---|---|
Bglap | 5′-GACACCATGAGGACCATCTTTC-3′/5′-CATGAAGGCTTTGTCAGACTCA-3′ |
Col1a1 | 5′-CCAATGGTGCTCCTGGTATT-3′/5′-GGTTCACCACTGTTACCCTT-3′ |
Runx2 | 5′-CTCTGATCGCCTCAGTGATTT-3′/5′-CTGCCTGGGATCTGTAATCTG-3′ |
Tbp | 5′-AGTGCCCAGCATCACTATTT-3′/5′-GGTCCATGATTCTCCCTTTCTT-3′ |
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Zhou, K.; Simonassi-Paiva, B.; Fehrenbach, G.; Yan, G.; Portela, A.; Pogue, R.; Cao, Z.; Fournet, M.B.; Devine, D.M. Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration. Molecules 2024, 29, 4208. https://doi.org/10.3390/molecules29174208
Zhou K, Simonassi-Paiva B, Fehrenbach G, Yan G, Portela A, Pogue R, Cao Z, Fournet MB, Devine DM. Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration. Molecules. 2024; 29(17):4208. https://doi.org/10.3390/molecules29174208
Chicago/Turabian StyleZhou, Keran, Bianca Simonassi-Paiva, Gustavo Fehrenbach, Guangming Yan, Alexandre Portela, Robert Pogue, Zhi Cao, Margaret Brennan Fournet, and Declan M. Devine. 2024. "Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration" Molecules 29, no. 17: 4208. https://doi.org/10.3390/molecules29174208
APA StyleZhou, K., Simonassi-Paiva, B., Fehrenbach, G., Yan, G., Portela, A., Pogue, R., Cao, Z., Fournet, M. B., & Devine, D. M. (2024). Investigating the Promising P28 Peptide-Loaded Chitosan/Ceramic Bone Scaffolds for Bone Regeneration. Molecules, 29(17), 4208. https://doi.org/10.3390/molecules29174208