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Article

Biotech Application of Exopolysaccharides from Curvularia brachyspora: Optimization of Production, Structural Characterization, and Biological Activity

by
Rafael Andrade Menolli
1,2,3,*,
Fernando Henrique Galvão Tessaro
4,
Alex Evangelista do Amaral
5,
Renan Henrique de Melo
1,
Jean Felipe dos Santos
2,3,
Marcello Iacomini
6,
Fhernanda Ribeiro Smiderle
2,3,* and
Rosiane Guetter Mello
2,3
1
Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel 85819-110, PR, Brazil
2
Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil
3
Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil
4
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo 05508-000, SP, Brazil
5
Unidade de Laboratório de Análises Clínicas, Universidade Federal de Santa Catarina, Florianópolis 88036-800, SC, Brazil
6
Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba 81531-980, PR, Brazil
*
Authors to whom correspondence should be addressed.
Molecules 2023, 28(11), 4356; https://doi.org/10.3390/molecules28114356
Submission received: 24 March 2023 / Revised: 18 May 2023 / Accepted: 22 May 2023 / Published: 26 May 2023
(This article belongs to the Special Issue Polysaccharides and Their Biological Activities)
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Abstract

C. brachyspora, a widespread dematiaceous fungus, was evaluated in this study to optimize the production of exopolysaccharides (CB-EPS). Optimization was performed using response surface methodology, and the best production yielded 75.05% of total sugar at pH 7.4, with 0.1% urea, after 197 h. The obtained CB-EPS showed typical signals of polysaccharides, which was confirmed by FT-IR and NMR. The HPSEC analysis indicated a polydisperse polymer, showing a non-uniform peak, with an average molar mass (Mw) of 24,470 g/mol. The major monosaccharide was glucose (63.9 Mol%), followed by mannose (19.7 Mol%), and galactose (16.4 Mol%). Methylation analysis encountered derivatives that indicated the presence of a β-d-glucan and a highly branched glucogalactomannan. CB-EPS was tested on murine macrophages to verify its immunoactivity, and the treated cells were able to produce TNF-α, IL-6, and IL-10. However, the cells did not produce superoxide anions or nitric oxide nor stimulated phagocytosis. The results demonstrated an indirect antimicrobial activity of macrophages by stimulating cytokines, showing another biotech applicability for the exopolysaccharides produced by C. brachyspora.
Keywords: dematiaceous fungi; response surface methodology; polysaccharide; fungal production; biological activity dematiaceous fungi; response surface methodology; polysaccharide; fungal production; biological activity

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MDPI and ACS Style

Menolli, R.A.; Tessaro, F.H.G.; do Amaral, A.E.; de Melo, R.H.; dos Santos, J.F.; Iacomini, M.; Smiderle, F.R.; Mello, R.G. Biotech Application of Exopolysaccharides from Curvularia brachyspora: Optimization of Production, Structural Characterization, and Biological Activity. Molecules 2023, 28, 4356. https://doi.org/10.3390/molecules28114356

AMA Style

Menolli RA, Tessaro FHG, do Amaral AE, de Melo RH, dos Santos JF, Iacomini M, Smiderle FR, Mello RG. Biotech Application of Exopolysaccharides from Curvularia brachyspora: Optimization of Production, Structural Characterization, and Biological Activity. Molecules. 2023; 28(11):4356. https://doi.org/10.3390/molecules28114356

Chicago/Turabian Style

Menolli, Rafael Andrade, Fernando Henrique Galvão Tessaro, Alex Evangelista do Amaral, Renan Henrique de Melo, Jean Felipe dos Santos, Marcello Iacomini, Fhernanda Ribeiro Smiderle, and Rosiane Guetter Mello. 2023. "Biotech Application of Exopolysaccharides from Curvularia brachyspora: Optimization of Production, Structural Characterization, and Biological Activity" Molecules 28, no. 11: 4356. https://doi.org/10.3390/molecules28114356

APA Style

Menolli, R. A., Tessaro, F. H. G., do Amaral, A. E., de Melo, R. H., dos Santos, J. F., Iacomini, M., Smiderle, F. R., & Mello, R. G. (2023). Biotech Application of Exopolysaccharides from Curvularia brachyspora: Optimization of Production, Structural Characterization, and Biological Activity. Molecules, 28(11), 4356. https://doi.org/10.3390/molecules28114356

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