Oxidative Stress and DNA Damage Effect of Dioscorea hispida Dennst. on Placental Tissues of Rats
Abstract
:1. Introduction
2. Results
2.1. UHPLC-ESI-MS Analysis
2.2. Determination of ROS Level
2.3. Lipid Peroxidation and Malondialdehyde (MDA) Quantification Assays
2.4. Superoxide Dismutase (SOD) Activity Assay
2.5. Determination of DNA Damage Using Comet Assay
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Plant Material and Extract Preparation
4.3. Total Polyphenols
4.4. UHPLC-ESI-MS Analysis
4.5. Animals and Experimental Design
4.6. Determination of ROS Level
4.7. Lipid Peroxidation and Malondialdehyde (MDA) Quantification Assays
4.8. Superoxide Dismutase (SOD) Activity Assay
4.9. Determination of DNA Damage Using Comet Assay
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Peak No | Retention Time Rt (min) | Compound | Formula | Selected Ion | m/z Observed | Error (ppm) |
---|---|---|---|---|---|---|
i. | 4.78 | (+)Syringavesinol-4-O-β-d-glucopyranoside-isomer | C28H35O13 | [M − H]− | 579.20844 | 2.11 |
ii. | 10.76 | (+)Syringavesinol-4-O-β-d-glucopyranoside-isomer | C28H35O13 | [M − H]− | 579.20807 | 1.47 |
iii. | 11.46 | Dioscin | C45H71O16 | [M − H]− | 867.47941 | 6.62 |
iv. | 12.06 | Disogenin-3,6-dione | C28H39O6 | [M − H]− | 471.27484 | 1.54 |
v. | 12.71 | Progenin III | C40H63O11 | [M − H]− | 719.43701 | 0.72 |
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Muhammad, H.; Bakar, T.A.S.T.A.; Yusery, M.F.A.; Awang, N.; Saad, W.M.M.; Ibnu Rasid, E.N.; Mahomoodally, M.F.; Omar, M.H. Oxidative Stress and DNA Damage Effect of Dioscorea hispida Dennst. on Placental Tissues of Rats. Molecules 2022, 27, 2190. https://doi.org/10.3390/molecules27072190
Muhammad H, Bakar TASTA, Yusery MFA, Awang N, Saad WMM, Ibnu Rasid EN, Mahomoodally MF, Omar MH. Oxidative Stress and DNA Damage Effect of Dioscorea hispida Dennst. on Placental Tissues of Rats. Molecules. 2022; 27(7):2190. https://doi.org/10.3390/molecules27072190
Chicago/Turabian StyleMuhammad, Hussin, Tengku Aideed Syah Tg Abu Bakar, Muhamad Faizul Adhzim Yusery, Norizah Awang, Wan Mazlina Md. Saad, Elda Nurafnie Ibnu Rasid, Mohamad Fawzi Mahomoodally, and Maizatul Hasyima Omar. 2022. "Oxidative Stress and DNA Damage Effect of Dioscorea hispida Dennst. on Placental Tissues of Rats" Molecules 27, no. 7: 2190. https://doi.org/10.3390/molecules27072190