Development of Optogenetic Dual-Switch System for Rewiring Metabolic Flux for Polyhydroxybutyrate Production
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains, Plasmids, Primers, and Culture Media
2.2. Plasmid and Strain Construction
2.3. Characterization of Light-Sensing Systems
2.4. PHB Fermentation
2.5. Analytical Method
2.6. Statistical Analysis
3. Results and Discussion
3.1. Characterization and Screening of Optogenetic Elements
3.2. Analysis of Crosstalk of Optogenetic Parts
3.3. Construction of Optogenetic Dual-Switch System
3.4. Dynamically Regulating Engineered E. coli for Enhancing PHB Production with Optogenetic Dual-Switch System
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Property | Photosensory System | |||
---|---|---|---|---|
RBS10–CcaS#10–CcaR | EL222 | YF1–FixJ–PhlF | LexRO | |
Sensory light | Activation in green light Repression in red light | Activation in blue light Repression in darkness | Activation in blue light Repression in darkness | Activation in blue light Repression in darkness |
Photosensory range | 10-fold | 7-fold | 10-fold | 1.3-fold |
Leakage | Negligible | Fewer leakage in green light | No leakage | — |
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Wang, S.; Luo, Y.; Jiang, W.; Li, X.; Qi, Q.; Liang, Q. Development of Optogenetic Dual-Switch System for Rewiring Metabolic Flux for Polyhydroxybutyrate Production. Molecules 2022, 27, 617. https://doi.org/10.3390/molecules27030617
Wang S, Luo Y, Jiang W, Li X, Qi Q, Liang Q. Development of Optogenetic Dual-Switch System for Rewiring Metabolic Flux for Polyhydroxybutyrate Production. Molecules. 2022; 27(3):617. https://doi.org/10.3390/molecules27030617
Chicago/Turabian StyleWang, Sumeng, Yue Luo, Wei Jiang, Xiaomeng Li, Qingsheng Qi, and Quanfeng Liang. 2022. "Development of Optogenetic Dual-Switch System for Rewiring Metabolic Flux for Polyhydroxybutyrate Production" Molecules 27, no. 3: 617. https://doi.org/10.3390/molecules27030617