Next Article in Journal
Classical vs. Non-Classical Cyclometalated Pt(II) Complexes
Next Article in Special Issue
Simultaneous Determination of 108 Pesticide Residues in Three Traditional Chinese Medicines Using a Modified QuEChERS Mixed Sample Preparation Method and HPLC-MS/MS
Previous Article in Journal
Fast Assembly of Metal Organic Framework UiO-66 in Acid-Base Tunable Deep Eutectic Solvent for the Acetalization of Benzaldehyde and Methanol
Previous Article in Special Issue
Autotoxicity of Endogenous Organic Acid Stress in Two Ganoderma lucidum Cultivars
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 27
Issue 21
10.3390/molecules27217247
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food

by
Wenyu Zhao
1,
Na Cui
1,
Yuan Huang
1,
Zihao Wang
1,
Peilong Wang
1,
Haijun Liang
2,
Ruiguo Wang
1,* and
Xiaoou Su
1,*
1
Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
CHINA FEED Magazine Agency, Beijing 100710, China
*
Authors to whom correspondence should be addressed.
Molecules 2022, 27(21), 7247; https://doi.org/10.3390/molecules27217247
Submission received: 8 October 2022 / Revised: 21 October 2022 / Accepted: 22 October 2022 / Published: 25 October 2022
(This article belongs to the Special Issue Application of LC-MS/MS in Biochemistry II)
Browse Figures
Versions Notes

Abstract

Hydroxylated polychlorinated biphenyls (OH-PCBs) are a group of metabolites biotransformed from polychlorinated biphenyls by animals with higher toxicities than their parent compounds. The present work developed and validated an analytical method for determinating penta-, hexa-, and hepta-chlorine substituted OH-PCBs in animal-derived food based on ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) with isotope-dilution. The target analytes were extracted with a 50% n-hexane/dichloromethane (v/v), purified by sulfuric acid-silica gel, and separated by 5% hydrated silica gel, achieving a final concentration of 100 times before injection to LC–MS/MS. The limits of detection (LOD) and quantification (LOQ) for target OH-PCBs were within the ranges of 0.003–0.010 μg/kg and 0.009–0.030 μg/kg, respectively. Average recoveries ranged between 76.7% and 116.5%, with relative standard deviations of less than 18.4%. The proposed method is simple, time-saving, sensitive, and accurate, making it a powerful tool for risk monitoring of OH-PCBs in animal-derived food.
Keywords: hydroxylated polychlorinated biphenyls; food; determination; metabolite; UPLC–MS/MS hydroxylated polychlorinated biphenyls; food; determination; metabolite; UPLC–MS/MS

Share and Cite

MDPI and ACS Style

Zhao, W.; Cui, N.; Huang, Y.; Wang, Z.; Wang, P.; Liang, H.; Wang, R.; Su, X. Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food. Molecules 2022, 27, 7247. https://doi.org/10.3390/molecules27217247

AMA Style

Zhao W, Cui N, Huang Y, Wang Z, Wang P, Liang H, Wang R, Su X. Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food. Molecules. 2022; 27(21):7247. https://doi.org/10.3390/molecules27217247

Chicago/Turabian Style

Zhao, Wenyu, Na Cui, Yuan Huang, Zihao Wang, Peilong Wang, Haijun Liang, Ruiguo Wang, and Xiaoou Su. 2022. "Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food" Molecules 27, no. 21: 7247. https://doi.org/10.3390/molecules27217247

APA Style

Zhao, W., Cui, N., Huang, Y., Wang, Z., Wang, P., Liang, H., Wang, R., & Su, X. (2022). Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food. Molecules, 27(21), 7247. https://doi.org/10.3390/molecules27217247

Article Metrics

Back to TopTop