Next Article in Journal
Evaluating the Functional Characteristics of Certain Insect Flours (Non-Defatted/Defatted Flour) and Their Protein Preparations
Next Article in Special Issue
Variations in the Chemical Composition of Essential Oils in Native Populations of Korean Thyme, Thymus quinquecostatus Celak.
Previous Article in Journal
Homology Modeling, Molecular Docking, Molecular Dynamic Simulation, and Drug-Likeness of the Modified Alpha-Mangostin against the β-Tubulin Protein of Acanthamoeba Keratitis
Previous Article in Special Issue
Neutrophil Immunomodulatory Activity of (−)-Borneol, a Major Component of Essential Oils Extracted from Grindelia squarrosa
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Chemical Diversity of Essential Oils from Korean Native Populations of Agastache rugosa (Korean Mint)

1
School of Natural Resources and Environmental Science, Kangwon National University, Chuncheon 24341, Gangwon-do, Korea
2
Agro-Food Research Institute, Gangwon Agricultural Research & Extension Service, Sinbuk, Chuncheon 24203, Gangwon-do, Korea
3
Department of Botany, Kongunadu Arts and Science College, Coimbatore 641029, Tamil Nadu, India
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(19), 6341; https://doi.org/10.3390/molecules27196341
Submission received: 6 September 2022 / Revised: 21 September 2022 / Accepted: 22 September 2022 / Published: 26 September 2022
(This article belongs to the Special Issue Chemical Composition and Bioactivities of Essential Oils)

Abstract

:
Agastache rugosa (baechohyang) is one of the most important aromatic plants native to the Republic of Korea. A. rugosa fragrance has been used to prepare incense since the Goryeo Dynasty in Korea. The present study aimed to explore the variation in the composition of essential oils from A. rugosa among native populations in Korea. The seeds of A. rugosa were collected from 90 different sites in Korea and seedlings were raised in the nursery. Essential oils were extracted from these populations by the steam distillation extraction method and their chemical compositions were analyzed by GC-MS. The yield of essential oils of A. rugosa ranged between 0.11% and 0.86%. A total of 204 components were identified from 90 populations of A. rugosa. Out of 204 components, 32 components were common in more than 40 individuals of A. rugosa and these 32 components were selected for principal component analysis (PCA). On the basis of the essential oil compositions, six chemotypes—estragole, pulegone, methyl eugenol, menthone, isopulegone, and nepetalactone—were distinguished according to their major components. As a result of the cluster analysis, 90 individuals of A. rugosa could be classified into three groups: estragole, methyl eugenol, and pulegone. A. rugosa exhibited significant chemical diversity among the individuals. The distribution of chemotypes is associated with the collection of seeds, suggesting that genetic diversity may influence the variations in the chemical compositions and concentrations within the species. This chemical diversity serves as the background to select cultivars for the cultivation and industrial applications of A. rugosa cultivars with high essential oil yield and concentration of its chemical components.

1. Introduction

Agastache rugosa (Fisch. & C. A. Mey.) Kuntze is an important aromatic edible plant in Korea that belongs to the Lamiaceae family. The A. rugosa plant is mainly found in East Asia, China, Japan, and Taiwan [1]. The common name of A. rugosa is Korean mint (baechohyang) and its aerial parts contain essential oils with a unique fragrance. Therefore, the leaves of A. rugosa are used to enhance the aroma and taste of Korean dishes, such as salads and soups [2,3]. In traditional Korean medicine, A. rugosa is used for the treatment of numerous diseases, especially anxiety, cholera, diarrhea, fever, and nausea [1,4]. Previously, several studies reported that A. rugosa contains a variety of bioactive metabolites that prevent inflammation, cardiovascular diseases, cancer, photoaging, adipogenesis, etc. [4,5,6,7,8].
Previously, few studies reported on the chemical composition of essential oils obtained from A. rugosa in the Republic of Korea [3,9,10]. This plant has a high developmental value as a flavoring crop for various industrial products. Major biologically active constituents of A. rugosa are characterized into two main metabolic classes: terpenoids and phenylpropanoids. The essential oil of A. rugosa is mainly composed of estragole, followed by D-limonene, menthone, and pulegone. However, a huge variation in the chemical composition of essential oils of A. rugosa has been reported, according to different geographical origins [3,6,11]. Knowledge of the chemical composition of essential oils from aromatic plants is an important quality measure for their commercial utilization.
Environmental factors play a critical role in determining the chemical polymorphism of plant essential oils [12]. In addition, the quantity and composition of essential oils from aromatic plants are mainly influenced by various factors such as genotype, season, stage of maturity, extraction technique, and drying [13,14]. Principal component analysis (PCA) is an important multivariate analysis method for grouping or clustering quantitative data based on the significance of the sample [11,15,16].
In this context, the present study aimed to provide information on the variation of essential oil compositions of Korean A. rugosa. For this purpose, seeds of A. rugosa were collected from 90 different regions in Korea, the plants were cultivated, and their essential oil compositions were analyzed using gas chromatography and mass spectrometry (GC-MS) analysis. Furthermore, PCA was performed to classify chemotypes in order to identify differences in regional species of A. rugosa in Korea.

2. Results and Discussion

2.1. Color and Yield of Essential Oils from A. rugosa Populations

A. rugosa is one of the important medicinal and aromatic plants in Korea. The essential oil obtained from this plant has been used for enhancing the aroma of Korean dishes. This oil is mainly composed of monoterpenoids, phenylpropanoids, and other aromatic components [4]. In this study, 90 Korean native A. rugosa populations were selected for essential oil analysis. Seeds collected from 90 individuals were cultivated in the same field under similar environmental conditions and then used for essential oil extraction (Figure 1). Essential oils were extracted from 90 individuals of A. rugosa by the steam distillation method. For this purpose, seeds of A. rugosa were collected from different cultivated regions in Korea. The yield percent (v/w) and the color of essential oils markedly varied according to the collection sites of the seeds (Table 1). The extraction yield of A. rugosa essential oils was a minimum of 0.11% and a maximum of 0.86%. The essential oil varied depending on the seed collection area. Furthermore, the color of the essential oils was classified into colorless, pale lemon, and lemon (Figure 2). Similar to our report, the yield of essential oil obtained from the aerial parts of A. rugosa ranged between 0.15 and 0.49% [3,17].

2.2. Chemical Composition of Essential Oils of A. rugosa Populations

In total, 204 chemical components were identified in the essential oils of 90 individuals of Korean A. rugosa based on the retention indices and mass spectral data (Supplementary Table S1). Among them, methyl chavicol (estragole), D-limonene, isopulegone, menthone, pulegone, β-caryophyllene, and β-cubebene were registered as major components in the essential oils of A. rugosa populations. The composition of essential oils of A. rugosa populations differed not only by their major components, but also by the number of different classes of components. Li et al. [18] identified 37 chemical components from the essential oil of A. rugosa, and the major components were methyl eugenol (50.1%), estragole (8.5%), eugenol (7.5%), thymol (3.6%), and pulegone (2.5%). A recent study reported that the most abundant component in the essential oil of A. rugosa was estragole (89.49%), followed by D-limonene (3.40%), menthone (1.80%), and pulegone (1.86%) [3]. Estragole was also the main component in the essential oil of A. ruogsa cultivated in Australia [19]. In other studies, menthone (48.8%) was reported as the most abundant component in the essential oil obtained from the leaves of A. rugosa cultivated in China [6,20]. Based on previous reports, A. rugosa is classified into three chemotypes: pulegone, estragole, and methyl eugenol [18]. These studies suggested that the variation in the essential oil composition may be influenced by various ecological and physiological factors. In addition, ontogenetic cues play a partial role in the varied proportions of essential oil components [11].
In the present study, 32 components were common in more than 40 individuals of A. rugosa. A. rugosa individuals collected from various sites were classified into six chemotypes according to their chemical components (Table 2). Among them, the estragole chemotype showed the highest number of A. rugosa (53 individuals), followed by the pulegone type (17 individuals), methyl eugenol type (11 individuals), menthone type (6 individuals), isopulegone type (2 individuals), and nepetalactone type (1 individual). Although the collected seeds were grown in the same environmental conditions, significant variations were observed in the essential oil compositions of 90 individuals. Hence, the data may reflect the genetic diversity within the species. It was reported that genetic diversity within the species plays a major role in essential oil compositions and concentrations [21,22,23]. Kang et al. [24] studied the genetic diversity of 65 accessions of A. rugosa germplasms using inter simple sequence repeat (ISSR) markers. The authors reported these accessions were grouped into two major clusters (A and B), and cluster A is subdivided into two sub-clusters. Furthermore, Dang et al. [25] compared morphological features and essential oil compositions in the pulegone and estragole chemotypes of A. rugosa. Previous studies also found that the morphological variations among different chemotypes were mainly in leaf type, trichome density, plant height, and internode length [26,27]. These studies clearly indicate that morphological and genetic variability among A. rugosa populations may influence the chemical diversity within species.

2.3. Principal Components Analysis of the Essential Oils of A. rugosa Populations

Multivariate data analysis has been used for the numerical taxonomic classification of plants based on their phenotypic, chemical, and molecular features. In particular, PCA and cluster analyses have been performed on the basis of the essential oil components in plants [11,23,28]. PCA was used to determine the variability in the chemical composition of essential oils obtained from 90 individuals of A. rugosa seeds collected from different regions of Korea. Statistical analysis was performed based on the data from the composition of essential oils of A. rugosa populations. Based on the principal component analysis of 32 essential oil components from 90 A. rugosa individuals, principal components PC1, PC2, PC3, and PC4 accounted for 70.97%, 20.91%, 3.74%, and 3.38% of the total variation, respectively. The main principal components, PC1 and PC2, can account for approximately 91.87% of the total variation. The dimension was reduced to two principal components (Table 3).
Table 4 shows the correlation between chemical components and principal components. The main component PC1 showed a relatively high correlation with the contents of pulegone (C13), β-pinene (C5), and menthone (C10), whereas it showed a low correlation with the content of estragole (C12). On the other hand, the principal component PC2 showed a high correlation with the contents of methyl eugenol (C18), β-caryophyllene (C20), and α-humulene (C21) components. Figure 3 shows the scatter plot of the major essential oil components on the plane coordinates using the principal component PC1 and PC2 values.
Using the scores of the principal components PC1 and PC2, A. rugosa populations have been expressed as a scatter plot, as shown in Figure 4. The principal component PC1 (high content of pulegone, l-β-pinene, and menthone) is correlated with A. rugosa individuals of AR2, AR6, AR7, AR9, AR14, AR17, AR21, AR35, AR36, AR37, AR38, AR39, AR40, AR41, AR42, AR43, AR44, AR54, AR57, AR65, AR66, AR69, AR70, and AR88. The principal component PC2 (high content of methyl eugenol, β-caryophyllene, and α-humulene) is correlated with A. rugosa individuals of AR45, AR46, AR47, AR48, AR49, AR50, AR53, AR84, and AR89 (Figure 4).
Zielińska et al. [11] studied the essential oil profiles of the progeny derived from different ages of mother plants of A. rugosa in cultivated plants and in vitro shoot cultures. A phenylpropanoid, estragole, was the most abundant component in A. rugosa essential oil. The quantitative variation among individuals was mainly in the concentration of estragole, menthone/isomenthone ratio in leaves, and pulegone in inflorescences. The authors suggested that the essential oil compositions of A. rugosa were highly dependent on the age of the mother plants. Zielińska and Matkowski [29] reviewed that the essential oil of A. rugosa is mainly dominated by estragole, but other monoterpene components such as limonene, menthone, isomenthone, and pulegone may also be present in high concentrations. Similar to our study, Li et al. [18] isolated the essential oils of A. rugosa aerial parts collected from Zhejiang, Hubei, and Henan provinces of China and found three different chemotypes, pulegone, estragole, and methyl eugenol, were major components, respectively. The morphological variations, essential oil components, and transcriptomic data between the two chemotypes of A. rugosa, pulegone and estragole, were also investigated [25].
The results of the correlation coefficients between 32 chemicals in the essential oils of Korean A. rugosa populations are presented in Supplementary Table S2. Among them, the chemical components that showed significance at the 1% probability level and had a correlation coefficient of 0.70 or higher were as follows: 1-octen-3-ol showed a high correlation coefficient with 3-octanone (0.822 **), 3-octanol (0.826 **), and l-β-pinene (0.743 **) components, 3-octanone with 3-octanol (0.795 **), and 3-octanol with β-pinene (0.745 **). β-Pinene showed a correlation with menthone (0.750 **) and pulegone (0.756 **). Isopulegone showed a high positive correlation coefficient with isopiperitenone (0.840 **). β-Bourbonene with β-elemene (0.793 **), methyl eugenol with α-humulene (0.718 **), and β-elemene with β-cubebene (0.711 **). In addition, β-caryophyllene showed the highest correlation coefficients with α-humulene (0.965 **) and β-cubebene (0.729 **). α-Humulene with β-cubebene (0.701 **), and γ-elemene with β-cadinene (0.808**), t-muurolol (0.720 **), and α-cadinol (0.733 **), and β-cadinene showed a positive correlation coefficient with α-cadinol (0.734 **). The biosynthetic pathways of β-caryophyllene and α-humulene are intimately associated because the compounds have the same chemical structures and molecular weights. On the other hand, the strongest negative correlation (−0.716 **) was observed between estragole and β-pinene components. Furthermore, the remaining components showed low correlations. The results demonstrate that the correlations can be used as an important outcome to understand the relationship between Korean native A. rugosa populations and their chemical components (Supplementary Table S2). Moreover, the data reveal that cultivars can be developed according to the content of the compounds. The results indicate that A. rugosa populations were categorized into three major chemotypes: estragole, pulegone, and methyl eugenol.
As a result of the cluster analysis, 90 individuals of A. rugosa could be classified into three groups according to their chemical composition, as shown in the dendrogram (Figure 5). Group I included most A. rugosa individuals with the highest estragole content in their essential oils. Group II contains individuals of A. rugosa with the highest methyl eugenol content and group III contains individuals of A. rugosa with the highest pulegone content. Furthermore, group III could be divided into three sub-groups according to the ratio of essential oil components. The chemical composition characteristics of each cluster are shown in Table 5. Dang et al. [25] reported that 46 genes were identified with the biosynthesis of estragole and pulegone. In these, the authors identified chavicol methyl transferase and limonene-3-hydroxylase in A. rugosa. Jang et al. [30] investigated the phenotypic expression and floral dimorphism in five Korean populations of A. rugosa and reported that three phenotypes were found according to their reproductive characteristics. The findings of this study suggest that different chemotypes among A. rugosa populations are not associated with environmental conditions. The changes in the composition of essential oils and the concentration of components may be associated with phenotypic and genetic characteristics of A. rugosa populations. In particular, genetic and environmental variations play a key role in phenotypic variation within the species [31].
Previous studies demonstrated that various edaphic and ecological factors influenced the essential oil yield and their chemical components in aromatic plants [23,32,33]. In the present study, the statistical analysis results suggest that the essential oil components are predominantly determined by the plant’s genotype, because all collected seeds were cultivated under similar environmental conditions. Furthermore, planting season may slightly influence the overall yield of essential oils and their compositions of A. rugosa populations.

3. Materials and Methods

3.1. Collection of A. rugosa Seeds and Their Cultivation

Korean domestic A. rugosa seeds were collected from 90 different regions of the Republic of Korea in 2019 (Table 1). The seeds were sown in 128-hole seedling trays (17 cm3, Seoul Bio Co., Ltd., Chungbuk, Korea) filled with horticultural topsoil in April 2019. The seedlings were grown for 36 days in a glass greenhouse at the Agricultural Research Institute, Gangwon-do Agricultural Research and Development Institute, where the proper temperature (23–25 °C day) and humidity were maintained. In early May, 3.5-leaf-bearing herbaceous seedlings were planted in two rows at 25 cm intervals with an area of 1650 m2 (Figure 1). The plants were grown until the flowering stage, which is known to contain the highest essential oil content. After that, aerial parts of all samples of A. rugosa were harvested at the blooming stage and immediately cut into 15 cm units used for the essential oil extraction.

3.2. Essential Oil Extraction

The essential oils were separately extracted from the aerial parts of 90 different A. rugosa individuals by steam distillation. The essential oil extraction was carried out with a 1 kg sample of A. rugosa for 1 h and 30 min using a steam distillation apparatus (EssenLab Plus, Hanil Lab Tech Co, Ltd., Yangju, Korea). The essential oil yield (%) was calculated as volume (mL) of extracted oil per 1 kg of fresh plant sample. After extraction, water and impurities in the extracted essential oil were removed using anhydrous sodium sulfate and stored at 4 °C for further analysis.

3.3. GC-MS Analysis

The GC-MS analysis of the essential oil of A. rugosa was performed by a Varian CP-3800 (GC)/Varian 1200 L (MS) (Varian, Palo Alto, CA, USA) equipped with a capillary column VF-5MS (Agilent, Santa Clara, CA, USA) polydimethylsiloxane (30 m × 0.25 mm × 0.25 µm). The GC oven temperature was programmed to increase from 50 °C (for 5 min) to 250 °C (for 3 min) at a rate of 5 °C/min, then increased to 300 °C at a rate of 20 °C/min, and the final temperature was kept for 5 min. The injector temperature was set at 250 °C and the ion source temperature was set at 280 °C. One µL of the sample was injected with a split ratio of 20:1, and helium was used as a carrier gas at a rate of 1 mL/min. For mass spectra analysis, the ionization voltage was set to 70 eV, and the mass range was set to 30–500 m/z. The components in the essential oil of A. rugosa were identified by comparing the mass spectrum data of the National Institute of Standards and Technology (NIST, 3.0) library and the retention indices (RI) relative to a homologous series of n-alkanes (C8–C20) with those reported in the literature [34].

3.4. Statistical Analysis

Prior to the statistical analysis, the data of essential oil components obtained from 90 individuals of A. rugosa were integrated and sorted in ascending order based on the RI value. Then, the common chemical components that appeared in 40 or more A. rugosa populations were extracted separately from the raw data. Statistical analysis was done based on the completely extracted data and then cluster analysis was performed. Principal component analysis (PCA) was performed to analyze multiple data on the content of essential oil components and to understand the relationship between the essential oil composition and the collection site of the seeds of A. rugosa. All statistical analyzes were performed using IBM SPSS ver. 24 (IBM Corp. Released 2016, Chicago, IL, USA).

4. Conclusions

The results reveal a significant chemical polymorphism within Korean native A. rugosa populations according to seed collection sites. Based on the essential oil compositions, 90 individuals of A. rugosa were classified into six chemotypes: estragole, pulegone, methyl eugenol, menthone, isopulegone, and nepetalactone. Furthermore, these 90 individuals of A. rugosa could be classified into three groups—estragole, methyl eugenol, and pulegone—according to PCA and cluster analysis. These results can extend our knowledge of the chemical diversity of A. rugosa populations, suggesting the view that essential components are excellent markers at the intraspecific level. Further genetic diversity studies of A. rugosa populations are warranted to understand the variation within the species.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules27196341/s1, Table S1. The area percent of 32 components in the essential oils of A. rugosa populations. Table S2. Correlation coefficients between 32 chemicals of essential oils from A. rugosa populations.

Author Contributions

Conceptualization, S.K.; methodology, S.K., M.H. and K.-Y.L.; formal analysis, M.H. and K.-Y.L.; investigation, M.H.; resources, K.-Y.L. and K.K.; data curation, M.H. and P.D.; writing—original draft preparation, P.D. and K.S.; writing—review and editing, S.K. and K.S.; supervision, S.K. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

This study was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ014506), Rural Development Administration, Korea.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Lee, Y.; Lim, H.W.; Ryu, I.W.; Huang, Y.H.; Park, M.; Chi, Y.M.; Lim, C.J. Anti-inflammatory, barrier-protective, and antiwrinkle properties of Agastache rugosa Kuntze in human epidermal keratinocytes. BioMed Res. Int. 2020, 2020, 1759067. [Google Scholar] [CrossRef] [PubMed]
  2. Bae, K.H. The Medicinal Plants of Korea; Kyo-Hak Publishing: Seoul, Korea, 2000; p. 432. [Google Scholar]
  3. Hong, M.J.; Jang, H.J.; Bo, S.; Kim, M.J.; Deepa, P.; Park, J.; Sowndhararajan, K.; Kim, S. Changes in Human Electroencephalographic Activity in Response to Agastache rugosa Essential Oil Exposure. Behav. Sci. 2022, 12, 238. [Google Scholar] [CrossRef] [PubMed]
  4. Seo, H.; Kim, C.; Kim, M.B.; Hwang, J.K. Anti-photoaging effect of Korean mint (Agastache rugosa Kuntze) extract on UVB-irradiated human dermal fibroblasts. Prev. Nutr. Food Sci. 2019, 24, 442–448. [Google Scholar] [CrossRef] [PubMed]
  5. Guo, X.; Cao, W.; Yao, J.; Yuan, Y.; Hong, Y.; Wang, X.; Xing, J. Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury. Mol. Med. Rep. 2015, 11, 2227–2233. [Google Scholar] [CrossRef] [PubMed]
  6. Gong, H.; He, L.; Li, S.; Zhang, C.; Ashraf, M.A. Antimicrobial, antibiofilm and antitumor activities of essential oil of Agastache rugosa from Xinjiang, China. Saudi J. Biol. Sci. 2016, 23, 524–530. [Google Scholar]
  7. Lim, H.W.; Lee, Y.; Huang, Y.H.; Yoon, J.Y.; Lee, S.H.; Kim, K.; Lim, C.J. Enhancement of skin antioxidant and anti-inflammatory potentials of Agastache rugosa leaf extract by probiotic bacterial fermentation in human epidermal keratinocytes. Microbiol. Biotechnol. Lett. 2017, 45, 35–42. [Google Scholar] [CrossRef]
  8. Hwang, J.M.; Lee, M.H.; Lee, J.H.; Lee, J.H. Agastache rugosa extract and its bioactive compound tilianin suppress adipogenesis and lipogenesis on 3t3-l1 cells. Appl. Sci. 2021, 11, 7679. [Google Scholar] [CrossRef]
  9. Kim, J.M. Flavoral essential oil components in the stems of Agastache rugosa for aromatherapy. J. Korean Soc. Food Cult. 2021, 36, 317–324. [Google Scholar]
  10. Lim, S.S.; Jang, J.M.; Park, W.T.; Uddin, M.R.; Chae, S.C.; Kim, H.H.; Park, S.U. Chemical composition of essential oils from flower and leaf of Korean mint, Agastache rugosa. Asian J. Chem. 2013, 25, 4361–4363. [Google Scholar] [CrossRef]
  11. Zielińska, S.; Dąbrowska, M.; Kozłowska, W.; Kalemba, D.; Abel, R.; Dryś, A.; Szumny, A.; Matkowski, A. Ontogenetic and trans-generational variation of essential oil composition in Agastache rugosa. Ind. Crops Prod. 2017, 97, 612–619. [Google Scholar] [CrossRef]
  12. Liu, W.; Yin, D.; Li, N.; Hou, X.; Wang, D.; Li, D.; Liu, J. Influence of environmental factors on the active substance production and antioxidant activity in Potentilla fruticosa L. and its quality assessment. Sci. Rep. 2016, 6, 28591. [Google Scholar] [CrossRef]
  13. Bakkali, F.; Averbeck, S.; Averbeck, D.; Idaomar, M. Biological effects of essential oils-A review. Food Chem. Toxicol. 2008, 46, 446–475. [Google Scholar] [CrossRef]
  14. Jug-Dujaković, M.; Ristić, M.; Pljevljakušić, D.; Dajić-Stevanović, Z.; Liber, Z.; Hančević, K.; Radić, T.; Satović, Z. High diversity of indigenous populations of dalmatian sage (Salvia officinalis L.) in essential-oil composition. Chem. Biodivers. 2012, 9, 2309–2323. [Google Scholar] [CrossRef]
  15. Judzentiene, A.; Budiene, J. Chemical polymorphism of essential oils of Artemisia vulgaris growing wild in Lithuania. Chem. Biodivers. 2018, 15, e1700257. [Google Scholar] [CrossRef]
  16. Valarezo, E.; Vullien, A.; Conde-Rojas, D. Variability of the Chemical Composition of the Essential Oil from the Amazonian Ishpingo Species (Ocotea quixos). Molecules 2021, 26, 3961. [Google Scholar] [CrossRef]
  17. Hong, M.J.; Kim, J.H.; Kim, H.Y.; Kim, M.J.; Kim, S. Chemical composition and biological activity of essential oil of Agastache rugosa (Fisch. & CA Mey.) O. Kuntze. Korean J. Med. Crop Sci. 2020, 28, 95–110. [Google Scholar]
  18. Li, H.Q.; Liu, Q.Z.; Liu, Z.L.; Du, S.S.; Deng, Z.W. Chemical composition and nematicidal activity of essential oil of Agastache rugosa against Meloidogyne incognita. Molecules 2013, 18, 4170–4180. [Google Scholar] [CrossRef]
  19. Yamani, H.; Mantri, N.; Morrison, P.D.; Pang, E. Analysis of the volatile organic compounds from leaves, flower spikes, and nectar of Australian grown Agastache rugosa. BMC Complement. Altern. Med. 2014, 14, 495. [Google Scholar] [CrossRef]
  20. Gong, H.; Li, S.; He, L.; Kasimu, R. Microscopic identification and in vitro activity of Agastache rugosa (Fisch. et Mey) from Xinjiang, China. BMC Complement. Altern. Med. 2017, 17, 95. [Google Scholar] [CrossRef]
  21. Gupta, A.K.; Mishra, R.; Singh, A.K.; Srivastava, A.; Lal, R.K. Genetic variability and correlations of essential oil yield with agro-economic traits in Mentha species and identification of promising cultivars. Ind. Crops Prod. 2017, 95, 726–733. [Google Scholar] [CrossRef]
  22. Leontaritou, P.; Lamari, F.N.; Papasotiropoulos, V.; Iatrou, G. Exploration of genetic, morphological and essential oil variation reveals tools for the authentication and breeding of Salvia pomifera subsp. calycina (Sm.) Hayek. Phytochemistry 2021, 191, 112900. [Google Scholar] [CrossRef]
  23. Badr, A.; El-Shazly, H.H.; Sakr, M.; Farid, M.M.; Hamouda, M.; Elkhateeb, E.; Ahmad, H.S. Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes. J. Genet. Eng. Biotechnol. 2021, 19, 166. [Google Scholar] [CrossRef]
  24. Kang, M.J.; Sundan, S.; Lee, G.A.; Ko, H.C.; Chung, J.W.; Huh, Y.C.; Gwag, J.G.; Oh, S.J.; Kim, Y.G.; Cho, G.T. Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers. Korean J. Plant Res. 2013, 26, 362–369. [Google Scholar]
  25. Dang, J.; Lin, G.; Liu, L.; Zhou, P.; Shao, Y.; Dai, S.; Sang, M.; Jiang, Z.; Liu, C.; Wu, Q. Comparison of Pulegone and Estragole chemotypes provides new insight into volatile oil biosynthesis of Agastache rugosa. Front. Plant Sci. 2022, 13, 771. [Google Scholar] [CrossRef]
  26. Czechowski, T.; Larson, T.R.; Catania, T.M.; Harvey, D.; Wei, C.; Essome, M.; Brown, G.D.; Graham, I.A. Detailed phytochemical analysis of high- and low artemisinin producing chemotypes of Artemisia annua. Front. Plant Sci. 2018, 9, 641. [Google Scholar] [CrossRef]
  27. Wêglarz, Z.; Kosakowska, O.; Przybył, J.L.; Pióro-Jabrucka, E.; Baczek, K. The quality of Greek oregano (O. vulgare L. subsp. hirtum (Link) Ietswaart) and common oregano (O. vulgare L. subsp. vulgare) Cultivated in the temperate climate of Central Europe. Foods 2020, 9, 1671. [Google Scholar]
  28. Bielecka, M.; Zielińska, S.; Pencakowski, B.; Stafiniak, M.; Ślusarczyk, S.; Prescha, A.; Matkowski, A. Age-related variation of polyphenol content and expression of phenylpropanoid biosynthetic genes in Agastache rugosa. Ind. Crops Prod. 2019, 141, e111743. [Google Scholar] [CrossRef]
  29. Zielińska, S.; Matkowski, A. Phytochemistry and bioactivity of aromatic and medicinal plants from the genus Agastache (Lamiaceae). Phytochem. Rev. 2014, 13, 391–416. [Google Scholar] [CrossRef]
  30. Jang, T.S.; Moon, H.K.; Hong, S.P. Sex expression, population structure, and floral dimorphism in a gynodioecious herb, Agastache rugosa (Lamiaceae) in Korea. Flora 2015, 215, 23–32. [Google Scholar] [CrossRef]
  31. Tsusaka, T.; Makino, B.; Ohsawa, R.; Ezura, H. Genetic and environmental factors influencing the contents of essential oil compounds in Atractylodes lancea. PLoS ONE 2019, 14, e0217522. [Google Scholar] [CrossRef]
  32. Akula, R.; Ravishankar, G.A. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal. Behav. 2011, 6, 1720–1731. [Google Scholar] [CrossRef] [PubMed]
  33. Sampaio, B.L.; Costa, F.B.D. Influence of abiotic environmental factors on the main constituents of the volatile oils of Tithonia diversifolia. Rev. Bras. Farmacogn. 2018, 28, 135–144. [Google Scholar] [CrossRef]
  34. Adams, R.P. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry; Allured Publishing Co., Ltd.: Carol Stream, IL, USA, 2007. [Google Scholar]
Figure 1. The cultivation of A. rugosa under field conditions.
Figure 1. The cultivation of A. rugosa under field conditions.
Molecules 27 06341 g001
Figure 2. The color of essential oils extracted from A. rugosa.
Figure 2. The color of essential oils extracted from A. rugosa.
Molecules 27 06341 g002
Figure 3. PCA scatter plot for each 32 chemicals from A. rugosa essential oils.
Figure 3. PCA scatter plot for each 32 chemicals from A. rugosa essential oils.
Molecules 27 06341 g003
Figure 4. Scatter plot of the 90 A. rugosa individuals.
Figure 4. Scatter plot of the 90 A. rugosa individuals.
Molecules 27 06341 g004
Figure 5. Dendrogram obtained by cluster analysis based on the essential oil components of A. rugosa populations.
Figure 5. Dendrogram obtained by cluster analysis based on the essential oil components of A. rugosa populations.
Molecules 27 06341 g005
Table 1. The extraction yield and color of essential oils from A. rugosa populations.
Table 1. The extraction yield and color of essential oils from A. rugosa populations.
No.Name
(Sampling Site)
Yield
(%)
ColorNo.Name
(Sampling Site)
Yield
(%)
Color
1AR1(Incheon)0.138Colorless46AR46(NAC27)0.140Pale lemon
2AR2(KNA1)0.189Lemon47AR47(NAC28)0.164Colorless
3AR3(KNA2)0.296Colorless48AR48(NAC29)0.269Pale lemon
4AR4(KNA3)0.200Colorless49AR49(NAC30)0.170Colorless
5AR5(KNA4)0.282Colorless50AR50(NAC31)0.225Colorless
6AR6(KNA5)0.217Colorless51AR51(Cheongju)0.426Colorless
7AR7(KNA6)0.261Colorless52AR52(Seoul)0.274Colorless
8AR8(KNA7)0.282Colorless53AR53(Chungnyeongsan)0.170Colorless
9AR9(KNA8)0.167Colorless54AR54(Taebaek)0.226Pale lemon
10AR10(KNA9)0.151Lemon55AR55(Hamyang)0.863Lemon
11AR11(KNA10)0.155Colorless56AR56(Ulleungdo)0.294Colorless
12AR12(KNA11)0.217Colorless57AR57(Dutasan)0.227Colorless
13AR13(KU1)0.121Colorless58AR58(Bukhansan)0.297Pale lemon
14AR14(KU2)0.176Lemon59AR59(Jeonju)0.397Colorless
15AR15(KU3)0.298Colorless60AR60(Namyangju)0.348Colorless
16AR16(KU4)0.163Colorless61AR61(Hwangmae-san)0.371Colorless
17AR17(KU5)0.153Colorless62AR62(Jinju)0.225Colorless
18AR18(KU6)0.249Colorless63AR63(Seoul)0.298Colorless
19AR19(KU7)0.261Colorless64AR64(Seoul)0.372Pale lemon
20AR20(KU8)0.238Colorless65AR65(Jirisan)0.258Pale lemon
21AR21(NAC2)0.259Pale lemon66AR66(Chuncheon)0.184Colorless
22AR22(NAC3)0.287Colorless67AR67(Seoul)0.282Lemon
23AR23(NAC4)0.108Colorless68AR68(Gohan)0.117Pale lemon
24AR24(NAC5)0.247Colorless69AR69(Hambaeksan)0.295Pale lemon
25AR25(NAC6)0.281Colorless70AR70(Taebaek)0.295Pale lemon
26AR26(NAC7)0.216Colorless71AR71(Seoul)0.329Pale lemon
27AR27(NAC8)0.250Colorless72AR72(Yeongcheon)0.322Colorless
28AR28(NAC9)0.235Colorless73AR73(Haman)0.425Colorless
29AR29(NAC10)0.247Colorless74AR74(Gyeongju)0.267Colorless
30AR30(NAC11)0.259Colorless75AR75(Gyeongju)0.343Colorless
31AR31(NAC12)0.290 Colorless76AR76(Jinju)0.168Colorless
32AR32(NAC13)0.200 Colorless77AR77(Hamyang)0.262Colorless
33AR33(NAC14)0.242 Pale lemon78AR78(Yeongcheon)0.281Colorless
34AR34(NAC15)0.239 Lemon79AR79(Pocheon)0.179Colorless
35AR35(NAC16)0.187 Pale lemon80AR80(Uiwang)0.343Colorless
36AR36(NAC17)0.160 Colorless81AR81(Pyeongnae)0.278Colorless
37AR37(NAC18)0.277Lemon82AR82(Cheonmasan)0.239Pale lemon
38AR38(NAC19)0.240 Colorless83AR83(Odaesan)0.457Colorless
39AR39(NAC20)0.324 Colorless84AR84(Ulleungdo)0.179Lemon
40AR40(NAC21)0.273 Pale lemon85AR85Yeoncheon)0.380Pale lemon
41AR41(NAC22)0.383 Colorless86AR86(Choansan)0.261Pale lemon
42AR42(NAC23)0.338Colorless87AR87(Changny-eong)0.270Colorless
43AR43(NAC24)0.255 Colorless88AR88(Balwangsan)0.353Colorless
44AR44(NAC25)0.440 Colorless89AR89(Jeongseon)0.225Pale lemon
45AR45(NAC26)0.181 Pale lemon90AR90(Seoul)0.314Colorless
KNA, Korea National Arboretum; KU, Korea University; NAC, National Agrobiodiversity Center.
Table 2. The chemotype classification of Korean A. rugosa populations.
Table 2. The chemotype classification of Korean A. rugosa populations.
ChemotypeSample Name
Estragole (53)AR3, AR5, AR8, AR10, AR11, AR12, AR15, AR16, AR18, AR19, AR20, AR22, AR23, AR24, AR25, AR26, AR27, AR28, AR29, AR30, AR31, AR32, AR33, AR34, AR51, AR52, AR55, AR56, AR58, AR59, AR60, AR61, AR62, AR63, AR64, AR67, AR68, AR71, AR72, AR73, AR74, AR75, AR76, AR77, AR78, AR79, AR80, AR81, AR82, AR85, AR86, AR87, AR90
Isopulegone (2)AR83, AR88
Menthone (6)AR6, AR9, AR17, AR35, AR36, AR42
Methyl eugenol (11)AR4, AR13, AR45, AR46, AR47, AR48, AR49, AR50, AR53, AR84, AR89
Nepetalactone (1)AR1
Pulegone (17)AR2, AR7, AR14, AR21, AR37, AR38, AR39, AR40, AR41, AR43, AR44, AR54, AR57, AR65, AR66, AR69, AR70
Table 3. PC scores of the 32 components in the essential oils of Korean A. rugosa populations.
Table 3. PC scores of the 32 components in the essential oils of Korean A. rugosa populations.
No.CodeCompound NamePrincipal Components
PC1PC2PC3PC4
1C11-Octen-3-ol0.540−0.1300.0290.296
2C23-Octanone0.4630.0460.0050.228
3C33-Octanol0.586−0.0820.0270.150
4C4β-Phellandrene0.507−0.1570.1640.331
5C5β-Pinene0.745−0.4180.1040.121
6C6D-Limonene0.5180.3800.1900.109
7C7Linalool0.370−0.2360.043−0.008
8C81-Octen-3-yl-acetate−0.2730.1810.0210.086
9C9d-2,8-p-Menthadien-1-ol0.500−0.2720.1070.149
10C10Menthone0.662−0.4440.1950.551
11C11Isopulegone0.186−0.0300.730−0.625
12C12Estragole−0.995−0.077−0.0570.002
13C13Pulegone0.753−0.582−0.269−0.140
14C14Piperitone0.405−0.2210.2200.341
15C15Isopiperitenone0.408−0.2010.529−0.571
16C16α-Copaene0.155−0.0980.0780.222
17C17β-Bourbonene0.4720.2420.1900.263
18C18Methyl eugenol0.3470.919−0.171−0.001
19C19β-Elemene0.2650.1420.0810.198
20C20β-Caryophyllene−0.0290.706−0.0580.230
21C21α-Humulene0.0450.774−0.0370.239
22C22β-Cubebene0.0930.5360.0260.210
23C23γ-Elemene0.0710.3060.1480.265
24C24Butylated hydroxytoluene0.1280.1460.025−0.094
25C25γ-Cadinene−0.1880.0460.237−0.059
26C26β-Cadinene0.1260.1520.1890.200
27C27γ-Muurolene−0.0570.3950.0070.110
28C28β-Caryophyllene oxide0.2780.0870.2130.164
29C29T-Cadinol0.4260.0600.1890.147
30C30T-Muurolol0.2690.1440.0880.247
31C31α-Cadinol0.4480.1570.1110.259
32C32Phytol0.0880.441−0.0150.128
% Variance70.96820.9063.7423.373
Cumulative % of variance70.96891.87195.61398.986
Table 4. Chemical characterization of the principal components in essential oils of local varieties of A. rugosa.
Table 4. Chemical characterization of the principal components in essential oils of local varieties of A. rugosa.
Principal ComponentClassCorresponding Characters
PCA1+Pulegone, β-pinene, menthone
Estragole
PCA2+Methyl eugenol, β-caryophyllene, α-humulene
Table 5. The different chemical characterization of the three groups for Korean A. rugosa populations.
Table 5. The different chemical characterization of the three groups for Korean A. rugosa populations.
GroupMajor ChemicalCharacterization
IEstragoleContent ratio of estragole in essential oil is the highest
IIMethyl eugenolThe highest ratio of methyl eugenol content
IIIPulegone
isopulegone
The highest ratio of pulegone and isopulegone contents
Pulegone
menthone
The highest ratio of pulegone and menthone contents (1:1)
PulegoneThe highest ratio of pulegone content
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Hong, M.; Deepa, P.; Lee, K.-Y.; Kim, K.; Sowndhararajan, K.; Kim, S. Chemical Diversity of Essential Oils from Korean Native Populations of Agastache rugosa (Korean Mint). Molecules 2022, 27, 6341. https://doi.org/10.3390/molecules27196341

AMA Style

Hong M, Deepa P, Lee K-Y, Kim K, Sowndhararajan K, Kim S. Chemical Diversity of Essential Oils from Korean Native Populations of Agastache rugosa (Korean Mint). Molecules. 2022; 27(19):6341. https://doi.org/10.3390/molecules27196341

Chicago/Turabian Style

Hong, Minji, Ponnuvel Deepa, Ki-Yeon Lee, Kyunghee Kim, Kandhasamy Sowndhararajan, and Songmun Kim. 2022. "Chemical Diversity of Essential Oils from Korean Native Populations of Agastache rugosa (Korean Mint)" Molecules 27, no. 19: 6341. https://doi.org/10.3390/molecules27196341

Article Metrics

Back to TopTop