Recombinant Human Annexin A5 Alleviated Traumatic-Brain-Injury Induced Intestinal Injury by Regulating the Nrf2/HO-1/HMGB1 Pathway
Abstract
:1. Introduction
2. Results
2.1. ANXA5 Reduced Intestinal Lesions Induced by TBI
2.2. ANXA5 Increased Mouse Body Weight after TBI
2.3. ANXA5 Inhibited TBI-Induced Intestinal Apoptosis
2.4. ANXA5 Alleviated TBI-Induced Intestinal Mucosal Damage
2.5. ANXA5 Mitigated Intestinal MMP-9 after TBI
2.6. ANXA5 Increased Anti-Inflammatory Cytokines and Reduced Pro-Inflammatory Cytokines after TBI
2.7. ANXA5 Inhibited Inflammatory Infiltration after TBI
2.8. ANXA5 Attenuated Intestinal Oxidative Stress after TBI
2.9. ANXA5 Reduced HMGB1 and Increased HO-1 and Nrf2 after TBI
3. Materials and Methods
3.1. Mice
3.2. Fluid Percussion Injury Model
3.3. Hematoxylin and Eosin Staining
3.4. Lipid Peroxidation Assay
3.5. Immunofluorescence Staining
3.6. Enzyme-Linked Immunosorbent Assay
3.7. TUNEL Staining
3.8. Western Blotting
3.9. Statistical Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Zhang, H.; Gao, Y.; Li, T.; Li, F.; Peng, R.; Wang, C.; Zhang, S.; Zhang, J. Recombinant Human Annexin A5 Alleviated Traumatic-Brain-Injury Induced Intestinal Injury by Regulating the Nrf2/HO-1/HMGB1 Pathway. Molecules 2022, 27, 5755. https://doi.org/10.3390/molecules27185755
Zhang H, Gao Y, Li T, Li F, Peng R, Wang C, Zhang S, Zhang J. Recombinant Human Annexin A5 Alleviated Traumatic-Brain-Injury Induced Intestinal Injury by Regulating the Nrf2/HO-1/HMGB1 Pathway. Molecules. 2022; 27(18):5755. https://doi.org/10.3390/molecules27185755
Chicago/Turabian StyleZhang, Hejun, Yalong Gao, Tuo Li, Fanjian Li, Ruilong Peng, Cong Wang, Shu Zhang, and Jianning Zhang. 2022. "Recombinant Human Annexin A5 Alleviated Traumatic-Brain-Injury Induced Intestinal Injury by Regulating the Nrf2/HO-1/HMGB1 Pathway" Molecules 27, no. 18: 5755. https://doi.org/10.3390/molecules27185755