Simultaneous UHPLC-MS Quantification of Catechins and Untargeted Metabolomic Profiling for Proof-of-Concept Authenticity Determination of Maytenus ssp. Samples
Abstract
:1. Introduction
2. Results and Discussion
2.1. Analytical Method Validation
2.2. Quantification and Metabolomics
3. Materials and Methods
3.1. Plant Material
3.2. Sample Extraction
3.3. Analytical Method
3.4. Untargeted Processing of Full-Scan Chromatograms
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | Standard Values | Calculated Values Catechin | Calculated Values Epicatechin |
---|---|---|---|
Homoscedasticity | 0.616 | 0.553 | 0.427 |
Correlation Coefficient (r) | 0.99 | 0.9971 | 0.9929 |
Determination Coefficient (r2) | - | 0.9986 | 0.9964 |
Significance of the Angular Coefficient | 6.12 | 5534 | 2223 |
Evaluation of the Linear Coefficient (intercept) | 4.30 | 2.00 | 0.00 |
Independence of Residues | 1.5 | 1.58 | 1.57 |
Normality of Residues | 0.908 | 0.937 | 0.962 |
Evaluation of Outliers | 3.00 | No outliers | No outliers |
Variation (%) Angular Coef. between Solvent and Plant Matrix | - | 1.72 | 2.5 |
Repeatability (RSD%) | <15 | 6.5 | 4.0 |
Intermediate Precision (RSD%) | <15 | 7.0 | 5.4 |
Accuracy (recovery %) | 80–110 | 98.5–109.33 | 97.90–103.12 |
Detection Limit (µg/mL) | - | 0.03 | 0.03 |
Quantification Limit (µg/mL) | - | 0.1 | 0.1 |
Sample Name | Description | Acquired in | Expiry Date | Catechin (mg/g) | Epicatechin (mg/g) |
---|---|---|---|---|---|
Mi | M. ilicifolia | CPQBA Campinas/SP | - | 2.0 | 4.4 |
Ma | M. aquifolium | CPQBA Campinas/SP | - | <LQ | 1.9 |
Hb | Maytenus hybrid | CPQBA Campinas/SP | - | 0.8 | 4.1 |
A | brand A (packaged) | Pharmacy Itapira/SP | Apr-19 | 1.0 | 2.3 |
B | brand B (packaged) | Pharmacy Itapira/SP | Apr-19 | <LQ | <LQ |
C | brand C (packaged) | Pharmacy Itapira/SP | Feb-19 | 0.3 | 1.1 |
D | brand D (bulk) | City Market Lindóia/SP | - | 0.4 | 0.9 |
E | brand E (bulk) | City Market Jacareí/SP | - | 1.3 | 5.5 |
F | brand F (bulk) | City Market Araraquara/SP | - | 0.7 | 5.2 |
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Duarte, R.S.; Antunes, E.R.M.; Sawaya, A.C.H.F. Simultaneous UHPLC-MS Quantification of Catechins and Untargeted Metabolomic Profiling for Proof-of-Concept Authenticity Determination of Maytenus ssp. Samples. Molecules 2022, 27, 5520. https://doi.org/10.3390/molecules27175520
Duarte RS, Antunes ERM, Sawaya ACHF. Simultaneous UHPLC-MS Quantification of Catechins and Untargeted Metabolomic Profiling for Proof-of-Concept Authenticity Determination of Maytenus ssp. Samples. Molecules. 2022; 27(17):5520. https://doi.org/10.3390/molecules27175520
Chicago/Turabian StyleDuarte, Rodolfo Santos, Elisa Ribeiro Miranda Antunes, and Alexandra Christine Helena Frankland Sawaya. 2022. "Simultaneous UHPLC-MS Quantification of Catechins and Untargeted Metabolomic Profiling for Proof-of-Concept Authenticity Determination of Maytenus ssp. Samples" Molecules 27, no. 17: 5520. https://doi.org/10.3390/molecules27175520