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Article

Development of a Lateral Flow Strip with a Positive Readout for the On-Site Detection of Aflatoxin B1

by 1,*,†, 2,†, 1, 2 and 1
1
Department of Preventive Medicine, Changzhi Medical College, Changzhi 046000, China
2
Department of Chemistry, Changzhi University, Changzhi 046011, China
*
Author to whom correspondence should be addressed.
Those authors contributed equally to this work.
Academic Editors: Rossella Svigelj and Gavino Sanna
Molecules 2022, 27(15), 4949; https://doi.org/10.3390/molecules27154949
Received: 25 June 2022 / Revised: 29 July 2022 / Accepted: 30 July 2022 / Published: 3 August 2022
Aflatoxin B1 is one of the contamination indicators for food safety monitoring. The rapid and effective assessment and determination of AFB1 in food is of great importance to dietary safety. The lateral flow assay shows advantages in its simplicity, and rapidity, and provides a visual readout, while the available lateral flow assay for AFB1 requires a competitive format that produces readings inversely proportional to the AFB1 concentration, which is counterintuitive and may lead to a potential misinterpretation of the results. Herein, we developed a positive readout aptamer-based lateral flow strip (Apt-strip) for the detection of AFB1. This Apt-strip relies on the competition between AFB1 and fluorescein-labeled complementary DNA strands (FAM-cDNA) for affinity binding to limited aptamers against AFB1 (AFB1-Apt). In the absence of AFB1, AFB1-Apt hybridizes with FAM-cDNA. No signal at the T-line of the Apt-strip was observed. In contrast, AFB1-Apt binds to AFB1 in the sample, and then a part of the FAM-cDNA is hybridized with the free AFB1-Apt, at which time the other unreacted FAM-cDNA is captured by A35-Apt on the T-line. The signal was observed. This method achieved fast detection of AFB1 with a detection limit (DL) of 0.1 ng/mL, positive readout, and increased sensitivity. View Full-Text
Keywords: positive readout; lateral flow assay; aptamer; aflatoxin B1 positive readout; lateral flow assay; aptamer; aflatoxin B1
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MDPI and ACS Style

Shen, K.; Hu, X.; Sun, L.; Han, C.; Yang, J. Development of a Lateral Flow Strip with a Positive Readout for the On-Site Detection of Aflatoxin B1. Molecules 2022, 27, 4949. https://doi.org/10.3390/molecules27154949

AMA Style

Shen K, Hu X, Sun L, Han C, Yang J. Development of a Lateral Flow Strip with a Positive Readout for the On-Site Detection of Aflatoxin B1. Molecules. 2022; 27(15):4949. https://doi.org/10.3390/molecules27154949

Chicago/Turabian Style

Shen, Kemin, Xiaoqin Hu, Linlin Sun, Chun Han, and Jianzhou Yang. 2022. "Development of a Lateral Flow Strip with a Positive Readout for the On-Site Detection of Aflatoxin B1" Molecules 27, no. 15: 4949. https://doi.org/10.3390/molecules27154949

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