Light and Potassium Improve the Quality of Dendrobium officinale through Optimizing Transcriptomic and Metabolomic Alteration
Abstract
:1. Introduction
2. Results
2.1. Phenotypic Changes in Light- or K-Treated D. officinale Pseudobulb
2.2. Metabolomic Changes in of Light- or K-Treated D. officinale Pseudobulb
2.3. Transcriptomic Changes in Light- or K-Treated D. officinale Pseudobulb
2.4. Identification of Key Genes Correlated to Flavonoid Biosynthesis via Coexpression Analysis
2.5. Gene-Metabolite Correlations Reveal Significant Interactions between Flavonoid-Related DEGs and DAMs
2.6. The Effect of JA on the Synthesis of Anthocyanins in D. officinale Pseudobulbs
3. Discussion
3.1. Light and K Regulate the Biosynthesis of Flavonoids in D. officinale
3.2. JA Promoted the Synthesis of Anthocyanin Which Contributed for the Purple Stem of D. officinale
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Metabolome Analyses
4.3. RNA Extraction, Library Construction, and Sequencing
4.4. Transcriptome Analyses
4.5. qRT-PCR Analysis
4.6. KEGG Enrichment Analysis of DEGs and Gene Coexpression Analysis
4.7. Transcriptome and Metabolome Joint Analysis
4.8. Determination of the Total Anthocyanin Content and Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Compounds | CAS | Log2FC | Compounds | CAS | Log2FC | ||
---|---|---|---|---|---|---|---|
PL/P | PK/P | PL/P | PK/P | ||||
Flavonoids | |||||||
Butin | 492-14-8 | −0.92 | −1.35 | Pinobanksin | 548-82-3 | −0.88 | −1.17 |
Naringenin chalcone | 73,692-50-9 | −0.95 | −1.43 | Hesperetin | 520-33-2 | −0.62 | −1.52 |
Genistein | 446-72-0 | −0.93 | −1.40 | Epigallocatechin-gallate | 989-51-5 | −1.13 | −1.66 |
Dihydrochrysin | 480-39-7 | −1.86 | −1.11 | (-)-Gallocatechin gallate | 4233-96-9 | −1.12 | −2.03 |
Fisetin | 528-48-3 | −0.70 | −0.67 | ||||
Narirutin | 14,259-46-2 | 0.73 | 0.90 | Quercetin-glc-rha | - | 0.88 | 1.97 |
Naringin | 10,236-47-2 | 0.75 | 0.68 | Isoquercitrin | 482-35-9 | 1.06 | 2.23 |
Isoluteolin-diglc | - | 1.34 | 1.89 | Quercetin-sop-rha | - | 0.89 | 1.54 |
Diosmetin-glc | - | 0.74 | 1.40 | Hyperin | 482-36-0 | 0.90 | 2.25 |
Isorhoifolin | 552-57-8 | 0.65 | 0.89 | Quercetin-rtn | 147,714-62-3 | 0.92 | 2.04 |
Apigenin-diara | - | 2.07 | 1.15 | Tricin (sinapoyl) glc | - | 0.73 | 0.63 |
Orientin-glc | - | 1.15 | 2.12 | Dihydrocharcone-glc | - | 0.78 | 1.14 |
Luteolin-ara | - | 0.78 | 0.66 | Hesperetin (malonyl) glc | - | 1.08 | 1.11 |
Luteolin-diglc | 29,428-58-8 | 1.56 | 1.98 | HydroxyKaempferol-diglc | 142,674-16-6 | 0.98 | 1.16 |
Astilbin | 29,838-67-3 | 0.77 | 0.68 | Hydroxykaempferol-rtn-glc | - | 0.93 | 1.66 |
Chrysoeriol-rtn | - | 0.69 | 1.56 | Hydroxykaempferol-glc | - | 1.37 | 2.45 |
Chrysoeriol-diglc | - | 0.61 | 1.02 | Malonylgenistin | 51,011-05-3 | 0.70 | 1.03 |
Quercetin-rtn-glc | - | 1.12 | 1.77 | Peonidin-diglc | 47,851-83-2 | 0.72 | 0.88 |
Rutin | 153-18-4 | 0.86 | 1.69 | Delphinidin-rtn-glc | - | 1.40 | 1.91 |
Quercetin-rob | 52,525-35-6 | 0.85 | 1.60 | Delphinidin (coumaroyl) glc | - | 0.98 | 1.96 |
JA and derivatives | |||||||
JA | 77,026-92-7 | 0.69 | - | 5′-Glucosyloxyjasmanic acid | 1.83 | 1.35 | |
JA-Ile | 120,330-93-0 | 2.26 | 0.94 | Dihydrojasmone | 1128-08-1 | 1.44 | 0.73 |
Cis-Jasmone | 488-10-8 | 0.72 | - |
GeneID | Gene | Annotation | Log2FC | p-Value | ||
---|---|---|---|---|---|---|
PL/P | PK/P | PL/P | PK/P | |||
Phenylpropanoid biosynthesis | ||||||
LOC110097226 | 4CL2 | 4-coumarate-CoA ligase 2 | - | −1.13 | - | 0.00 |
LOC110093998 | BBE22 | berberine bridge enzyme-like 22 | - | −0.70 | - | 0.00 |
LOC110113575 | C4H | trans-cinnamate 4-monooxygenase | - | −1.47 | - | 0.00 |
LOC110102215 | CSE | caffeoylshikimate esterase | 1.57 | 0.99 | 0.00 | 0.03 |
LOC110100929 | OMT | Tricetin 3′,4′,5′-O-trimethyltransferase | 0.62 | - | 0.00 | - |
LOC110097445 | PER51 | peroxidase 51-like | −2.55 | −2.07 | 0.00 | 0.00 |
LOC110095178 | PER65 | peroxidase 65 | −0.58 | −1.22 | 0.02 | 0.00 |
LOC110095989 | UFGT1 | glucosyltransferase | 0.77 | 1.29 | 0.04 | 0.00 |
Flavonoid biosynthesis | ||||||
LOC110100597 | BZ1 | anthocyanidin 3-O-glucosyltransferase | 1.69 | 1.72 | 0.00 | 0.00 |
LOC110103723 | ANS | anthocyanidin synthase | 2.40 | 1.91 | 0.00 | 0.00 |
LOC110113809 | CHS8 | chalcone synthase 8 | 1.61 | 1.94 | 0.00 | 0.00 |
LOC110101655 | DFR | dihydroflavonol-4-reductase | 0.88 | 1.79 | 0.10 | 0.00 |
LOC110097388 | F3H | flavanone 3-hydroxylase | 1.54 | 1.83 | 0.00 | 0.00 |
LOC110113268 | CYP75B3 | flavonoid 3′-monooxygenase CYP75B3-like | 1.05 | 1.87 | 0.01 | 0.00 |
LOC110103762 | F3′5′H1 | flavonoid 3′,5′-hydroxylase 1 | 0.94 | 0.88 | 0.05 | 0.00 |
JA synthesis | ||||||
LOC110093045 | AOC3 | allene oxide cyclase, chloroplastic-like | 0.71 | 0.53 | 0.00 | 0.04 |
LOC110103009 | AOS2 | allene oxide synthase2 | 0.87 | 0.91 | 0.00 | 0.00 |
LOC110108695 | AOS2L | allene oxide synthase 2-like | 0.64 | 0.55 | 0.00 | 0.00 |
LOC110096989 | OPR11 | Putative 12-oxophytodienoate reductase 11 | 0.86 | 1.34 | 0.00 | 0.00 |
LOC110108000 | LOX5.1 | lipoxygenase 5.1 | 2.71 | - | 0.06 | - |
LOC110108011 | LOX5.2 | lipoxygenase 5.2 | - | 0.69 | - | 0.01 |
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Jia, Y.; Liu, J.; Xu, M.; Chen, G.; Tan, M.; Xiang, Z. Light and Potassium Improve the Quality of Dendrobium officinale through Optimizing Transcriptomic and Metabolomic Alteration. Molecules 2022, 27, 4866. https://doi.org/10.3390/molecules27154866
Jia Y, Liu J, Xu M, Chen G, Tan M, Xiang Z. Light and Potassium Improve the Quality of Dendrobium officinale through Optimizing Transcriptomic and Metabolomic Alteration. Molecules. 2022; 27(15):4866. https://doi.org/10.3390/molecules27154866
Chicago/Turabian StyleJia, Yue, Juan Liu, Mengyao Xu, Guihong Chen, Mingpu Tan, and Zengxu Xiang. 2022. "Light and Potassium Improve the Quality of Dendrobium officinale through Optimizing Transcriptomic and Metabolomic Alteration" Molecules 27, no. 15: 4866. https://doi.org/10.3390/molecules27154866