Biotransformation of Benzoate to 2,4,6-Trihydroxybenzophenone by Engineered Escherichia coli
Abstract
:1. Introduction
2. Results
2.1. Identification of 2,4,6-TriHB (5) by Using the High-Resolution LC-ESI-QTOF-MS/MS
2.2. Localisation of 2,4,6-TriHB (5)
2.3. Structural Confirmation of 2,4,6-TriHB (5) by Using SIRIUS
Molecular Formula Annotation from the Positive Mode Mass Data
2.4. Molecular Formula and Structural Annotation from the Negative Mode Mass Data
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Enzymatic Preparation of 2,4,6-TriHB (5)
4.3. Construction of Plasmid pETDuet-BadA-GmBPS
4.4. Bioconversion of Benzoate (1) to 2,4,6-TriHB (5)
4.5. Metabolite Extraction from the Cell Pellets
4.6. Extraction of the Secreted Metabolites from the Culture Medium
4.7. Metabolite Detection by Using the High-Resolution LC-ESI-QTOF-MS/MS
4.8. Identification of 2,4,6-TriHB (5) Using an in Silico Mass Prediction Tool
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Klamrak, A.; Nabnueangsap, J.; Nualkaew, N. Biotransformation of Benzoate to 2,4,6-Trihydroxybenzophenone by Engineered Escherichia coli. Molecules 2021, 26, 2779. https://doi.org/10.3390/molecules26092779
Klamrak A, Nabnueangsap J, Nualkaew N. Biotransformation of Benzoate to 2,4,6-Trihydroxybenzophenone by Engineered Escherichia coli. Molecules. 2021; 26(9):2779. https://doi.org/10.3390/molecules26092779
Chicago/Turabian StyleKlamrak, Anuwatchakij, Jaran Nabnueangsap, and Natsajee Nualkaew. 2021. "Biotransformation of Benzoate to 2,4,6-Trihydroxybenzophenone by Engineered Escherichia coli" Molecules 26, no. 9: 2779. https://doi.org/10.3390/molecules26092779