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Article

Garcinielliptone G from Garcinia subelliptica Induces Apoptosis in Acute Leukemia Cells

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School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
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School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
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Faculty of Pharma-Science, Teikyo University, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Enrique Barrajon
Molecules 2021, 26(9), 2422; https://doi.org/10.3390/molecules26092422
Received: 21 February 2021 / Revised: 15 April 2021 / Accepted: 19 April 2021 / Published: 21 April 2021
(This article belongs to the Section Natural Products Chemistry)
Cytotoxicity and apoptosis-inducing properties of compounds isolated from Garcinia subelliptica leaves were investigated. The hexane-soluble portion of MeOH extracts of G. subelliptica leaves that showed cytotoxic activity was separated to yield seven compounds 17. Chemical structure analysis using NMR spectroscopy and mass spectrometry confirmed that compound 1 was canophyllol, and compounds 27 were garcinielliptones N, O, J, G, F, and garcinielliptin oxide, respectively. Among them, garcinielliptone G (5) showed growth inhibition by causing apoptosis in THP-1 and Jurkat cells derived from human acute monocytic leukemia and T lymphocyte cells, respectively. Apoptosis induced by garcinielliptone G (5) was demonstrated by the detection of early apoptotic cells with fluorescein-labeled Annexin V and increases in cleaved caspase-3 and cleaved PARP protein levels. However, the addition of caspase inhibitor Z-VAD-FMK did not affect growth arrest or apoptosis induction. These results suggest that garcinielliptone G (5) can induce both caspase-3 activation and caspase-independent apoptosis. Therefore, garcinielliptone G (5) may be a potential candidate for acute leukemia treatment. View Full-Text
Keywords: Garcinia subelliptica; garcinielliptone G; apoptosis; human acute leukemia cell lines Garcinia subelliptica; garcinielliptone G; apoptosis; human acute leukemia cell lines
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MDPI and ACS Style

Yun, Y.; Shioura, M.; Hitotsuyanagi, Y.; Yotsumoto, S.; Takahashi, Y.; Aoyagi, Y.; Kinoshita, T.; Takeya, K.; Inoue, H. Garcinielliptone G from Garcinia subelliptica Induces Apoptosis in Acute Leukemia Cells. Molecules 2021, 26, 2422. https://doi.org/10.3390/molecules26092422

AMA Style

Yun Y, Shioura M, Hitotsuyanagi Y, Yotsumoto S, Takahashi Y, Aoyagi Y, Kinoshita T, Takeya K, Inoue H. Garcinielliptone G from Garcinia subelliptica Induces Apoptosis in Acute Leukemia Cells. Molecules. 2021; 26(9):2422. https://doi.org/10.3390/molecules26092422

Chicago/Turabian Style

Yun, YoungSook, Mariko Shioura, Yukio Hitotsuyanagi, Satoshi Yotsumoto, Yuji Takahashi, Yutaka Aoyagi, Takeshi Kinoshita, Koichi Takeya, and Hideshi Inoue. 2021. "Garcinielliptone G from Garcinia subelliptica Induces Apoptosis in Acute Leukemia Cells" Molecules 26, no. 9: 2422. https://doi.org/10.3390/molecules26092422

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