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Review

Genetically Encodable Scaffolds for Optimizing Enzyme Function

by 1,2, 1,2,3 and 1,2,3,*
1
Synthetic Biology for Clinical and Technological Innovation, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore
2
Synthetic Biology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive, Singapore 117599, Singapore
3
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597, Singapore
*
Author to whom correspondence should be addressed.
Academic Editor: Rudy J. Richardson
Molecules 2021, 26(5), 1389; https://doi.org/10.3390/molecules26051389
Received: 10 February 2021 / Revised: 27 February 2021 / Accepted: 1 March 2021 / Published: 4 March 2021
Enzyme engineering is an indispensable tool in the field of synthetic biology, where enzymes are challenged to carry out novel or improved functions. Achieving these goals sometimes goes beyond modifying the primary sequence of the enzyme itself. The use of protein or nucleic acid scaffolds to enhance enzyme properties has been reported for applications such as microbial production of chemicals, biosensor development and bioremediation. Key advantages of using these assemblies include optimizing reaction conditions, improving metabolic flux and increasing enzyme stability. This review summarizes recent trends in utilizing genetically encodable scaffolds, developed in line with synthetic biology methodologies, to complement the purposeful deployment of enzymes. Current molecular tools for constructing these synthetic enzyme-scaffold systems are also highlighted. View Full-Text
Keywords: protein shells; synthetic enzymology; synthetic biology; protein scaffold; nucleic acid scaffold protein shells; synthetic enzymology; synthetic biology; protein scaffold; nucleic acid scaffold
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MDPI and ACS Style

Tan, Y.Q.; Xue, B.; Yew, W.S. Genetically Encodable Scaffolds for Optimizing Enzyme Function. Molecules 2021, 26, 1389. https://doi.org/10.3390/molecules26051389

AMA Style

Tan YQ, Xue B, Yew WS. Genetically Encodable Scaffolds for Optimizing Enzyme Function. Molecules. 2021; 26(5):1389. https://doi.org/10.3390/molecules26051389

Chicago/Turabian Style

Tan, Yong Quan, Bo Xue, and Wen Shan Yew. 2021. "Genetically Encodable Scaffolds for Optimizing Enzyme Function" Molecules 26, no. 5: 1389. https://doi.org/10.3390/molecules26051389

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