α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials
Abstract
:1. Introduction
2. Results
2.1. Isolation of α-Amyrin and β-Amyrin from Leaves of C. hindsii
2.2. Identification of α-Amyrin and β-Amyrin by GC-MS and ESI-MS
2.3. Elucidation of α-Amyrin and β-Amyrin by NMR
2.4. Quantitative Analysis of α-Amyrin and β-Amyrin in C. hindsii Leaves
2.5. Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase of α-Amyrin and β-Amyrin from C. hindsii
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Preparation of Plant Extract
4.3. Fractionation of Ethyl Acetate Extract
4.4. Identification α-Amyrin and β-Amyrin by Gas Chromatography-Mass Spectrometry (GC-MS)
4.5. Electrospray Ionization-Mass Spectrometry (ESI-MS) Analysis
4.6. Nuclear Magnetic Resonance (NMR) Data of α-Amyrin and β-Amyrin
4.7. Xanthine Oxidase (XO) Inhibitory Activity
4.8. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Free Radical Scavenging Activity
4.9. 2,20-Azinobis (3-Ethylbenzothiazoline-6-sulfonic Acid) (ABTS) Cation Scavenging Activity
4.10. Tyrosinase Inhibition
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Time (min) | Peak Area (% of Total) | Compounds | Formula | Molecular Weight (g/mol) | Chemical Class |
---|---|---|---|---|---|
28.92 | 40.61 | β-Amyrin | C30H50O | 426.70 | Triterpene |
29.53 | 59.10 | α-Amyrin | C30H50O | 426.70 | Triterpene |
Position | β-Amyrin | α-Amyrin | Position | β-Amyrin | α-Amyrin |
---|---|---|---|---|---|
1 | 38.60 | 38.80 | 16 | 26.20 | 26.60 |
2 | 27.20 | 27.30 | 17 | 32.70 | 33.80 |
3 | 79.00 | 79.10 | 18 | 47.20 | 59.10 |
4 | 39.80 | 38.80 | 19 | 46.80 | 39.70 |
5 | 55.20 | 55.20 | 20 | 31.10 | 39.60 |
6 | 18.40 | 18.40 | 21 | 34.70 | 31.30 |
7 | 32.50 | 32.90 | 22 | 37.10 | 40.00 |
8 | 41.70 | 40.00 | 23 | 28.10 | 28.10 |
9 | 47.60 | 47.70 | 24 | 15.60 | 15.70 |
10 | 37.00 | 36.90 | 25 | 15.50 | 15.60 |
11 | 23.70 | 23.30 | 26 | 16.80 | 17.40 |
12 | 121.70 | 124.40 | 27 | 26.00 | 23.40 |
13 | 145.20 | 139.60 | 28 | 28.40 | 28.80 |
14 | 42.80 | 41.50 | 29 | 33.30 | 16.90 |
15 | 26.90 | 28.10 | 30 | 23.50 | 21.40 |
Content of Mixture (g/kg Dry Weight) | Ratio (%) | Contents (g/kg Dry Weight) | ||
---|---|---|---|---|
10.75 | α-Amyrin | β-Amyrin | α-Amyrin | β-Amyrin |
69.60 | 30.40 | 7.48 | 3.27 |
Sample | Antioxidant Activity | XO IC50 (µg/mL) | Tyrosinase IC50 (µg/mL) | |
---|---|---|---|---|
DPPH IC50 (µg/mL) | ABTS IC50 (µg/mL) | |||
α-Amyrin and β-Amyrin | 125.55 ± 0.98 a | 155.28 ± 1.01 a | 258.22 ± 2.28 a | 178.85 ± 3.28 a |
Allopurinol | - | 7.58 ± 1.29 b | ||
Kojic acid | - | - | 15.55 ± 2.28 b | |
BHT | 8.22 ± 0.89 b | 53.40 ± 1.52 b | - | - |
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Viet, T.D.; Xuan, T.D.; Anh, L.H. α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials. Molecules 2021, 26, 7248. https://doi.org/10.3390/molecules26237248
Viet TD, Xuan TD, Anh LH. α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials. Molecules. 2021; 26(23):7248. https://doi.org/10.3390/molecules26237248
Chicago/Turabian StyleViet, Tran Duc, Tran Dang Xuan, and La Hoang Anh. 2021. "α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials" Molecules 26, no. 23: 7248. https://doi.org/10.3390/molecules26237248