Red Light-Emitting Water-Soluble Luminescent Iridium-Containing Polynorbornenes: Synthesis, Characterization and Oxygen Sensing Properties in Biological Tissues In Vivo
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis of Polymers P1–P4
2.2. Photophysical Properties of Polymers P1–P4
2.3. Biological Properties of Polymers P1–P4
3. Materials and Methods
3.1. Instruments and Characterization
3.2. Synthesis of Polymers
3.3. In Vitro Cytotoxicity Assay and Cellular Uptake Study
3.4. In Vivo Animal Study
3.5. Immunohistochemical (IHC) Staining for Hypoxia
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Polymer | λmaxabs/nm (log ε) in CH2Cl2 | λmaxem/nm (CH2Cl2) | Quantum Yield, % (in CH2Cl2) | Quantum Yield, % (in Water) | Chromaticity Coordinates in the CIE Diagram (x; y) | ||
---|---|---|---|---|---|---|---|
(a) | (b) | (a) | (b) | ||||
P1 | 297 sh (4.29), 344 (3.97), 476 (3.60), 550 (3.26) | 618 | 10.0 | 2.7 | 3.8 | 2.8 | 0.66; 0.34 |
P2 | 300 sh (4.45), 353 (4.29), 472 (3.91), 525 (3.90) | 657 | 2.5 | 0.5 | 0.5 | 0.3 | 0.71; 0.29 |
P3 | 299 sh (4.08), 346 (3.75), 477 (2.95), 552 (2.59) | 618 | 9.3 | 3.6 | 2.7 | 2.0 | 0.66; 0.34 |
P4 | 302 sh (4.18), 353 (4.04), 471 (3.66), 527 (3.65) | 657 | 2.6 | 0.5 | 0.4 | 0.2 | 0.71; 0.29 |
P1 | P3 | |
---|---|---|
τ0, μs | 1.5 ± 0.07 | 1.5 ± 0.08 |
τ, μs (τ in BSA, μs) | 1.1 ± 0.06 (1.2 ± 0.07) | 1.3 ± 0.06 (1.2 ± 0.06) |
Dynamic range r = τ0/τ | 1.36 ± 0.072 | 1.15 ± 0.07 |
Photon count rate (for τ) | 5890 | 5600 |
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Bochkarev, L.N.; Parshina, Y.P.; Gracheva, Y.V.; Kovylina, T.A.; Lermontova, S.A.; Klapshina, L.G.; Konev, A.N.; Lopatin, M.A.; Lukina, M.M.; Komarova, A.D.; et al. Red Light-Emitting Water-Soluble Luminescent Iridium-Containing Polynorbornenes: Synthesis, Characterization and Oxygen Sensing Properties in Biological Tissues In Vivo. Molecules 2021, 26, 6349. https://doi.org/10.3390/molecules26216349
Bochkarev LN, Parshina YP, Gracheva YV, Kovylina TA, Lermontova SA, Klapshina LG, Konev AN, Lopatin MA, Lukina MM, Komarova AD, et al. Red Light-Emitting Water-Soluble Luminescent Iridium-Containing Polynorbornenes: Synthesis, Characterization and Oxygen Sensing Properties in Biological Tissues In Vivo. Molecules. 2021; 26(21):6349. https://doi.org/10.3390/molecules26216349
Chicago/Turabian StyleBochkarev, Leonid N., Yulia P. Parshina, Yana V. Gracheva, Tatyana A. Kovylina, Svetlana A. Lermontova, Larisa G. Klapshina, Aleksey N. Konev, Mikhail A. Lopatin, Maria M. Lukina, Anastasia D. Komarova, and et al. 2021. "Red Light-Emitting Water-Soluble Luminescent Iridium-Containing Polynorbornenes: Synthesis, Characterization and Oxygen Sensing Properties in Biological Tissues In Vivo" Molecules 26, no. 21: 6349. https://doi.org/10.3390/molecules26216349