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Article

Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (Betula pendula Roth) and Black Birch (Betula obscura Kotula) Barks by FT-IR and HPLC-PDA

1
Department of Medical Chemistry, Medical University of Lublin, 4A Chodźki Str., 20-093 Lublin, Poland
2
Independent Laboratory of Behavioral Studies, Medical University of Lublin, 4A Chodźki Str., 20-093 Lublin, Poland
3
Department of Analytical Chemistry, Medical University of Lublin, 4A Chodźki Str., 20-093 Lublin, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Anna Petruczynik
Molecules 2021, 26(15), 4633; https://doi.org/10.3390/molecules26154633
Received: 13 July 2021 / Revised: 27 July 2021 / Accepted: 28 July 2021 / Published: 30 July 2021
(This article belongs to the Special Issue Natural Products: Isolation, Identification and Biological Activity)
Silver birch, Betula pendula Roth, is one of the most common trees in Europe. Due to its content of many biologically active substances, it has long been used in medicine and cosmetics, unlike the rare black birch, Betula obscura Kotula. The aim of the study was therefore to compare the antioxidant properties of extracts from the inner and outer bark layers of both birch trees towards the L929 line treated with acetaldehyde. Based on the lactate dehydrogenase test and the MTT test, 10 and 25% concentrations of extracts were selected for the antioxidant evaluation. All extracts at tested concentrations reduced the production of hydrogen peroxide, superoxide anion radical, and 25% extract decreased malonic aldehyde formation in acetaldehyde-treated cells. The chemical composition of bark extracts was accessed by IR and HPLC-PDA methods and surprisingly, revealed a high content of betulin and lupeol in the inner bark extract of B. obscura. Furthermore, IR analysis revealed differences in the chemical composition of the outer bark between black and silver birch extracts, indicating that black birch may be a valuable source of numerous biologically active substances. Further experiments are required to evaluate their potential against neuroinflammation, cancer, viral infections, as well as their usefulness in cosmetology. View Full-Text
Keywords: Betula pendula Roth; Betula obscura Kotula; silver birch; black birch; pentacyclic triterpenes Betula pendula Roth; Betula obscura Kotula; silver birch; black birch; pentacyclic triterpenes
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MDPI and ACS Style

Ostapiuk, A.; Kurach, Ł.; Strzemski, M.; Kurzepa, J.; Hordyjewska, A. Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (Betula pendula Roth) and Black Birch (Betula obscura Kotula) Barks by FT-IR and HPLC-PDA. Molecules 2021, 26, 4633. https://doi.org/10.3390/molecules26154633

AMA Style

Ostapiuk A, Kurach Ł, Strzemski M, Kurzepa J, Hordyjewska A. Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (Betula pendula Roth) and Black Birch (Betula obscura Kotula) Barks by FT-IR and HPLC-PDA. Molecules. 2021; 26(15):4633. https://doi.org/10.3390/molecules26154633

Chicago/Turabian Style

Ostapiuk, Aleksandra, Łukasz Kurach, Maciej Strzemski, Jacek Kurzepa, and Anna Hordyjewska. 2021. "Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (Betula pendula Roth) and Black Birch (Betula obscura Kotula) Barks by FT-IR and HPLC-PDA" Molecules 26, no. 15: 4633. https://doi.org/10.3390/molecules26154633

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