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Article

Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line

by
Deepthi Venkatachalapathy
1,
Chandan Shivamallu
2,*,
Shashanka K. Prasad
2,
Gopenath Thangaraj Saradha
2,
Parthiban Rudrapathy
3,
Raghavendra G. Amachawadi
4,
Sharanagouda S. Patil
5,
Asad Syed
6,
Abdallah M. Elgorban
6,
Ali H. Bahkali
6,
Shiva Prasad Kollur
7,* and
Kanthesh M. Basalingappa
1,*
1
Division of Molecular Biology, Faculty of Life Science, JSS Academy of Higher Education & Research, Mysuru 570015, India
2
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru 570105, India
3
Malabar Cancer Centre, Department of Clinical Laboratory Services & Translational Research, Thalassery, Kannur 670103, India
4
Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5606, USA
5
ICAR, National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Yelahanka, Bengaluru 560064, India
6
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
7
Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru 570026, India
*
Authors to whom correspondence should be addressed.
Molecules 2021, 26(15), 4593; https://doi.org/10.3390/molecules26154593
Submission received: 26 June 2021 / Revised: 14 July 2021 / Accepted: 19 July 2021 / Published: 29 July 2021
(This article belongs to the Special Issue Anticancer Drug Discovery and Development)
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Abstract

The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and exhibit anti-carcinogenic properties. This study aims to evaluate the anti-cancerous properties of these plant crude extracts on human liver cancer HepG2 cells. The leaves of Camellia sinensis, Withania somnifera and the seeds of Vitis vinifera were collected and methanolic extracts were prepared. Then, these extracts were subjected to DPPH, α- amylase assays to determine the antioxidant properties. A MTT assay was performed to investigate the viability of the extracts of HepG2 cells, and the mode of cell death was detected by Ao/EtBr staining and flow cytometry with PI Annexin- V FITC dual staining. Then, the protein expression of BAX and BCl2 was studied using fluorescent dye to determine the regulation of the BAX and BCl2 genes. We observed that all the three extracts showed the presence of bioactive compounds such as polyphenols or phytochemicals. The W. somnifera bioactive compounds were found to have the highest anti-proliferative activity on human liver cancer cells.
Keywords: Camellia sinensis; Withania somnifera; Vitis vinifera; anti-carcinogenic; antioxidants; HepG2 cell line Camellia sinensis; Withania somnifera; Vitis vinifera; anti-carcinogenic; antioxidants; HepG2 cell line

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MDPI and ACS Style

Venkatachalapathy, D.; Shivamallu, C.; Prasad, S.K.; Thangaraj Saradha, G.; Rudrapathy, P.; Amachawadi, R.G.; Patil, S.S.; Syed, A.; Elgorban, A.M.; Bahkali, A.H.; et al. Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line. Molecules 2021, 26, 4593. https://doi.org/10.3390/molecules26154593

AMA Style

Venkatachalapathy D, Shivamallu C, Prasad SK, Thangaraj Saradha G, Rudrapathy P, Amachawadi RG, Patil SS, Syed A, Elgorban AM, Bahkali AH, et al. Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line. Molecules. 2021; 26(15):4593. https://doi.org/10.3390/molecules26154593

Chicago/Turabian Style

Venkatachalapathy, Deepthi, Chandan Shivamallu, Shashanka K. Prasad, Gopenath Thangaraj Saradha, Parthiban Rudrapathy, Raghavendra G. Amachawadi, Sharanagouda S. Patil, Asad Syed, Abdallah M. Elgorban, Ali H. Bahkali, and et al. 2021. "Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line" Molecules 26, no. 15: 4593. https://doi.org/10.3390/molecules26154593

APA Style

Venkatachalapathy, D., Shivamallu, C., Prasad, S. K., Thangaraj Saradha, G., Rudrapathy, P., Amachawadi, R. G., Patil, S. S., Syed, A., Elgorban, A. M., Bahkali, A. H., Kollur, S. P., & Basalingappa, K. M. (2021). Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line. Molecules, 26(15), 4593. https://doi.org/10.3390/molecules26154593

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