Next Article in Journal
Influencing Factors on the Physicochemical Characteristics of Tea Polysaccharides
Next Article in Special Issue
Jabuticaba (Myrciaria jaboticaba) Peel as a Sustainable Source of Anthocyanins and Ellagitannins Delivered by Phospholipid Vesicles for Alleviating Oxidative Stress in Human Keratinocytes
Previous Article in Journal
Amino Acid Profile and Biological Properties of Silk Cocoon as Affected by Water and Enzyme Extraction
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 26
Issue 11
10.3390/molecules26113452
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia

by
Amna Ben Hassine
1,
Gabriele Rocchetti
2,
Leilei Zhang
2,
Biancamaria Senizza
2,
Gökhan Zengin
3,
Mohamad Fawzi Mahomoodally
4,
Mossadok Ben-Attia
5,
Youssef Rouphael
6,*,
Luigi Lucini
2,* and
Safia El-Bok
1
1
Laboratory of Biodiversity, Biotechnologies and Climate Change (LR11/ES09), Department of Biology, Faculty of Sciences of Tunis, University of Tunis El-Manar, Tunis 2092, Tunisia
2
Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy
3
Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, 42130 Konya, Turkey
4
Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit 230, Mauritius
5
Environment Biomonitoring Laboratory (LR01/ES14), Bizerta Faculty of Sciences, University of Carthage, Zarzouna 7021, Tunisia
6
Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
*
Authors to whom correspondence should be addressed.
Molecules 2021, 26(11), 3452; https://doi.org/10.3390/molecules26113452
Submission received: 23 April 2021 / Revised: 3 June 2021 / Accepted: 4 June 2021 / Published: 7 June 2021
(This article belongs to the Special Issue Integration between Food Chemistry and Health in Focus)
Browse Figures
Versions Notes

Abstract

Lupin seeds can represent a valuable source of phenolics and other antioxidant compounds. In this work, a comprehensive analysis of the phytochemical profile was performed on seeds from three Lupinus species, including one cultivar (Lupinus albus) and two wild accessions (Lupinus cossentinii and Lupinus luteus), collected from the northern region of Tunisia. Untargeted metabolomic profiling allowed to identify 249 compounds, with a great abundance of phenolics and alkaloids. In this regard, the species L. cossentinii showed the highest phenolic content, being 6.54 mg/g DW, followed by L. luteus (1.60 mg/g DW) and L. albus (1.14 mg/g DW). The in vitro antioxidant capacity measured by the ABTS assay on seed extracts ranged from 4.67 to 17.58 mg trolox equivalents (TE)/g, recording the highest values for L. albus and the lowest for L. luteus. The DPPH radical scavenging activity ranged from 0.39 to 3.50 mg TE/g. FRAP values varied between 4.11 and 5.75 mg TE/g. CUPRAC values for lupin seeds ranged from 7.20 to 8.95 mg TE/g, recording the highest for L. cossentinii. The results of phosphomolybdenum assay and metal chelation showed similarity between the three species of Lupinus. The acetylcholinesterase (AChE) inhibition activity was detected in each methanolic extract analyzed with similar results. Regarding the butyrylcholinesterase (BChE) enzyme, it was weakly inhibited by the Lupinus extracts; in particular, the highest activity values were recorded for L. albus (1.74 mg GALAE/g). Overall, our results showed that L. cossentinii was the most abundant source of polyphenols, consisting mainly in tyrosol equivalents (5.82 mg/g DW). Finally, significant correlations were outlined between the phenolic compounds and the in vitro biological activity measured, particularly when considering flavones, phenolic acids and lower-molecular-weight phenolics.
Keywords: foodomics; untargeted profiling; radical scavenging; acetylcholine esterase inhibition; functional components; Lupinus; bioactive compounds; UHPLC-QTOF-MS foodomics; untargeted profiling; radical scavenging; acetylcholine esterase inhibition; functional components; Lupinus; bioactive compounds; UHPLC-QTOF-MS

Share and Cite

MDPI and ACS Style

Ben Hassine, A.; Rocchetti, G.; Zhang, L.; Senizza, B.; Zengin, G.; Mahomoodally, M.F.; Ben-Attia, M.; Rouphael, Y.; Lucini, L.; El-Bok, S. Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia. Molecules 2021, 26, 3452. https://doi.org/10.3390/molecules26113452

AMA Style

Ben Hassine A, Rocchetti G, Zhang L, Senizza B, Zengin G, Mahomoodally MF, Ben-Attia M, Rouphael Y, Lucini L, El-Bok S. Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia. Molecules. 2021; 26(11):3452. https://doi.org/10.3390/molecules26113452

Chicago/Turabian Style

Ben Hassine, Amna, Gabriele Rocchetti, Leilei Zhang, Biancamaria Senizza, Gökhan Zengin, Mohamad Fawzi Mahomoodally, Mossadok Ben-Attia, Youssef Rouphael, Luigi Lucini, and Safia El-Bok. 2021. "Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia" Molecules 26, no. 11: 3452. https://doi.org/10.3390/molecules26113452

APA Style

Ben Hassine, A., Rocchetti, G., Zhang, L., Senizza, B., Zengin, G., Mahomoodally, M. F., Ben-Attia, M., Rouphael, Y., Lucini, L., & El-Bok, S. (2021). Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia. Molecules, 26(11), 3452. https://doi.org/10.3390/molecules26113452

Article Metrics

Back to TopTop