Next Article in Journal
C-11, a New Antiepileptic Drug Candidate: Evaluation of the Physicochemical Properties and Impact on the Protective Action of Selected Antiepileptic Drugs in the Mouse Maximal Electroshock-Induced Seizure Model
Next Article in Special Issue
Natural Compounds Isolated from Stachybotrys chartarum Are Potent Inhibitors of Human Protein Kinase CK2
Previous Article in Journal
Application of Co-Culture Technology to Enhance Protease Production by Two Halophilic Bacteria, Marinirhabdus sp. and Marinobacter hydrocarbonoclasticus
Previous Article in Special Issue
The Onset of Systemic Oxidative Stress Associated with the Accumulation of Lipid Peroxidation Product Acrolein in the Skin of Patients with Small-Vessel Vasculitis
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 26
Issue 11
10.3390/molecules26113143
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Communication

Proteomic Analysis of Zeb1 Interactome in Breast Carcinoma Cells

by
Sergey E. Parfenyev
1,
Sergey V. Shabelnikov
1,
Danila Y. Pozdnyakov
1,
Olga O. Gnedina
1,
Leonid S. Adonin
2,
Nickolai A. Barlev
1,2 and
Alexey G. Mittenberg
1,*
1
Institute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, Russia
2
Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(11), 3143; https://doi.org/10.3390/molecules26113143
Submission received: 8 December 2020 / Revised: 20 May 2021 / Accepted: 21 May 2021 / Published: 24 May 2021
(This article belongs to the Collection Molecular Medicine)
Browse Figures
Review Reports Versions Notes

Abstract

Breast cancer is the most frequently diagnosed malignant neoplasm and the second leading cause of cancer death among women. Epithelial-to-mesenchymal Transition (EMT) plays a critical role in the organism development, providing cell migration and tissue formation. However, its erroneous activation in malignancies can serve as the basis for the dissemination of cancer cells and metastasis. The Zeb1 transcription factor, which regulates the EMT activation, has been shown to play an essential role in malignant transformation. This factor is involved in many signaling pathways that influence a wide range of cellular functions via interacting with many proteins that affect its transcriptional functions. Importantly, the interactome of Zeb1 depends on the cellular context. Here, using the inducible expression of Zeb1 in epithelial breast cancer cells, we identified a substantial list of novel potential Zeb1 interaction partners, including proteins involved in the formation of malignant neoplasms, such as ATP-dependent RNA helicase DDX17and a component of the NURD repressor complex, CTBP2. We confirmed the presence of the selected interactors by immunoblotting with specific antibodies. Further, we demonstrated that co-expression of Zeb1 and CTBP2 in breast cancer patients correlated with the poor survival prognosis, thus signifying the functionality of the Zeb1–CTBP2 interaction.
Keywords: breast cancer; Zeb1; metastasis; epithelial to mesenchymal transition (EMT) breast cancer; Zeb1; metastasis; epithelial to mesenchymal transition (EMT)

Share and Cite

MDPI and ACS Style

Parfenyev, S.E.; Shabelnikov, S.V.; Pozdnyakov, D.Y.; Gnedina, O.O.; Adonin, L.S.; Barlev, N.A.; Mittenberg, A.G. Proteomic Analysis of Zeb1 Interactome in Breast Carcinoma Cells. Molecules 2021, 26, 3143. https://doi.org/10.3390/molecules26113143

AMA Style

Parfenyev SE, Shabelnikov SV, Pozdnyakov DY, Gnedina OO, Adonin LS, Barlev NA, Mittenberg AG. Proteomic Analysis of Zeb1 Interactome in Breast Carcinoma Cells. Molecules. 2021; 26(11):3143. https://doi.org/10.3390/molecules26113143

Chicago/Turabian Style

Parfenyev, Sergey E., Sergey V. Shabelnikov, Danila Y. Pozdnyakov, Olga O. Gnedina, Leonid S. Adonin, Nickolai A. Barlev, and Alexey G. Mittenberg. 2021. "Proteomic Analysis of Zeb1 Interactome in Breast Carcinoma Cells" Molecules 26, no. 11: 3143. https://doi.org/10.3390/molecules26113143

APA Style

Parfenyev, S. E., Shabelnikov, S. V., Pozdnyakov, D. Y., Gnedina, O. O., Adonin, L. S., Barlev, N. A., & Mittenberg, A. G. (2021). Proteomic Analysis of Zeb1 Interactome in Breast Carcinoma Cells. Molecules, 26(11), 3143. https://doi.org/10.3390/molecules26113143

Article Metrics

Back to TopTop