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Open AccessArticle

Characterization of Free and Bound Phenolic Acids and Flavonoid Aglycones in Rosa rugosa Thunb. Leaves and Achenes Using LC–ESI–MS/MS–MRM Methods

Chair and Department of Pharmaceutical Botany, Medical University, 1 Chodźki Street, 20-093 Lublin, Poland
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Academic Editor: Monika Waksmundzka-Hajnos
Molecules 2020, 25(8), 1804; https://doi.org/10.3390/molecules25081804
Received: 20 March 2020 / Revised: 8 April 2020 / Accepted: 10 April 2020 / Published: 15 April 2020
(This article belongs to the Special Issue Chromatographic Science of Natural Products)
Fast and reliable determination of polyphenols is a quite common goal during investigation of new plant materials and herbal products, their standardization, quality control, or chemo-taxonomical studies. The aim of this study was to develop and validate methods based on the application of reversed phase liquid chromatography/electrospray ionization triple quadrupole mass spectrometry (LC-ESI-MS/MS) using multiple reaction monitoring (MRM) for comprehensive quantitative and qualitative analysis of phenolic acids and flavonoid aglycones. LC-MS/MS-MRM protocols were applied for the determination of free and bound phenolics in a series of plant samples prepared from leaves and achenes (true fruits) of Japanese rose (Rosa rugosa Thunb.). The presence of large amount of phenolic compounds was detected in rose leaves (786.44 µg/g and 14.46 µg/g of phenolic acids and flavonoid aglycones, respectively). Isoferulic acid and five aglycones were revealed for the first time in this plant material. Moreover, 15 phenolic acids and six aglycones were found in the rose achenes, including eight phenolic acids and four aglycones that had not been previously reported in this rose organ. It was shown that leaves and achenes may constitute an industrially relevant source of phenolic compounds for potential commercial use in pharmaceutical, food, or cosmetic industry. View Full-Text
Keywords: liquid chromatography mass spectrometry; validation; Rosaceae; rose leaves; rose true fruit; flavonoid aglycones; phenolic acids liquid chromatography mass spectrometry; validation; Rosaceae; rose leaves; rose true fruit; flavonoid aglycones; phenolic acids
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MDPI and ACS Style

Olech, M.; Pietrzak, W.; Nowak, R. Characterization of Free and Bound Phenolic Acids and Flavonoid Aglycones in Rosa rugosa Thunb. Leaves and Achenes Using LC–ESI–MS/MS–MRM Methods. Molecules 2020, 25, 1804.

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