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Open AccessArticle

Scrophularia Tenuipes Coss and Durieu: Phytochemical Composition and Biological Activities

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Laboratory of Molecular Toxicology, Faculty of Nature and Life Science, University of Mohamed Seddik Benyahia, 18000 Jijel, Algeria
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Laboratory of Phytochemistry and Physical-Chemical and Biological Analyses, Department of Chemistry, Faculty of Exact Sciences, University of Mentouri Constantine, 25000 Constantine, Algeria
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LAQV-REQUIMTE Department of Chemistry & Organic Chemistry, Natural Products and Food Stuffs Research Unit, University of Aveiro, 3810-193 Aveiro, Portugal
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Laboratory of Biomolecule and Plant Breeding, Life Science and Nature Department, Faculty of Exact Science and Life Science and Nature, University of Larbi Ben Mhidi, 4000 Oum El Bouaghi, Algeria
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Laboratory of Biotechnology Environment and Health, University of Mohamed Seddik Benyahia, 18000 Jijel, Algeria
*
Author to whom correspondence should be addressed.
Academic Editors: Salvatore Genovese and Bruno Botta
Molecules 2020, 25(7), 1647; https://doi.org/10.3390/molecules25071647
Received: 18 January 2020 / Revised: 5 March 2020 / Accepted: 30 March 2020 / Published: 3 April 2020
Scrophularia tenuipes is an Algerian-Tunisian endemic species, which has not been studied yet. Ethyl acetate (EA) and n-butanol (Bu) fractions obtained from Scrophularia tenuipes were investigated for their health benefit properties, in particular with respect to in vivo/in vitro anti-inflammatory and antioxidant activities, as well as their potential to inhibit key enzymes with impact in diabetes (α-glucosidase and α-amylase). The fractions had a distinct phytochemical composition, of which EA was richer in total phenolic compounds (225 mg GAE/g) and mostly composed of the phenylethanoid acetyl martynoside. Compared to EA, Bu had higher amounts of total flavonoids, and according to the result obtained from UHPLC-DAD-ESI-MSn analysis, harpagoside (iridoid) was its major phytochemical. EA fraction was quite promising with regard to the in vivo (at 200 mg/kg, po) anti-inflammatory effect (62% and 52% for carrageenan-induced rat paw edema and xylene-induced ear edema tests, respectively), while Bu fraction exhibited a stronger antioxidant capacity in all tests (IC50 = 68 µg/mL, IC50 = 18 µg/mL, IC50 = 18 µg/mL and A0.50 = 43 µg/mL for DPPH, ABTS•+, O2•− scavenging assays and cupric-reducing antioxidant capacity method, respectively). Both fractions also showed a strong effect against α-amylase enzyme (IC50 = 8 µg/mL and 10 µg/mL for EA and Bu fraction, respectively). View Full-Text
Keywords: Scrophularia tenuipes; UHPLC-ESI-DAD-MSn; phenolic; iridoids; phenylethanoid; anti-inflammatory; antioxidant; antidiabetic Scrophularia tenuipes; UHPLC-ESI-DAD-MSn; phenolic; iridoids; phenylethanoid; anti-inflammatory; antioxidant; antidiabetic
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Chaibeddra, Z.; Akkal, S.; Ouled-Haddar, H.; Silva, A.M.S.; Zellagui, A.; Sebti, M.; Cardoso, S.M. Scrophularia Tenuipes Coss and Durieu: Phytochemical Composition and Biological Activities. Molecules 2020, 25, 1647.

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