Next Article in Journal
Cytotoxic Activity of Organotin(IV) Derivatives with Triazolopyrimidine Containing Exocyclic Oxygen Atoms
Previous Article in Journal
Telodendrimer-Based Macromolecular Drug Design using 1,3-Dipolar Cycloaddition for Applications in Biology
Article

Characterization of a Flavonoid 3’/5’/7-O-Methyltransferase from Citrus reticulata and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products

by 1,2,3,†, 1,2,3,†, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3 and 1,2,3,*
1
College of Agriculture & Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China
2
Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China
3
The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2020, 25(4), 858; https://doi.org/10.3390/molecules25040858
Received: 18 January 2020 / Revised: 9 February 2020 / Accepted: 12 February 2020 / Published: 15 February 2020
(This article belongs to the Section Natural Products Chemistry)
O-methylation of flavonoids is an important modification reaction that occurs in plants. O-methylation contributes to the structural diversity of flavonoids, which have several biological and pharmacological functions. In this study, an O-methyltransferase gene (CrOMT2) was isolated from the fruit peel of Citrus reticulata, which encoding a multifunctional O-methyltransferase and could effectively catalyze the methylation of 3’-, 5’-, and 7-OH of flavonoids with vicinal hydroxyl substitutions. Substrate preference assays indicated that this recombinant enzyme favored polymethoxylated flavones (PMF)-type substrates in vitro, thereby providing biochemical evidence for the potential role of the enzyme in plants. Additionally, the cytotoxicity of the methylated products from the enzymatic catalytic reaction was evaluated in vitro using human gastric cell lines SGC-7901 and BGC-823. The results showed that the in vitro cytotoxicity of the flavonoids with the unsaturated C2-C3 bond was increased after being methylated at position 3’. These combined results provide biochemical insight regarding CrOMT2 in vitro and indicate the in vitro cytotoxicity of the products methylated by its catalytic reaction. View Full-Text
Keywords: citrus; flavonoids; O-methyltransferase; bioactivity; cytotoxicity citrus; flavonoids; O-methyltransferase; bioactivity; cytotoxicity
Show Figures

Graphical abstract

MDPI and ACS Style

Liu, X.; Wang, Y.; Chen, Y.; Xu, S.; Gong, Q.; Zhao, C.; Cao, J.; Sun, C. Characterization of a Flavonoid 3’/5’/7-O-Methyltransferase from Citrus reticulata and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products. Molecules 2020, 25, 858. https://doi.org/10.3390/molecules25040858

AMA Style

Liu X, Wang Y, Chen Y, Xu S, Gong Q, Zhao C, Cao J, Sun C. Characterization of a Flavonoid 3’/5’/7-O-Methyltransferase from Citrus reticulata and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products. Molecules. 2020; 25(4):858. https://doi.org/10.3390/molecules25040858

Chicago/Turabian Style

Liu, Xiaojuan; Wang, Yue; Chen, Yezhi; Xu, Shuting; Gong, Qin; Zhao, Chenning; Cao, Jinping; Sun, Chongde. 2020. "Characterization of a Flavonoid 3’/5’/7-O-Methyltransferase from Citrus reticulata and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products" Molecules 25, no. 4: 858. https://doi.org/10.3390/molecules25040858

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop