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Open AccessReview

PNA Clamping in Nucleic Acid Amplification Protocols to Detect Single Nucleotide Mutations Related to Cancer

Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
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Academic Editor: Eylon Yavin
Molecules 2020, 25(4), 786; https://doi.org/10.3390/molecules25040786 (registering DOI)
Received: 23 December 2019 / Revised: 30 January 2020 / Accepted: 2 February 2020 / Published: 12 February 2020
(This article belongs to the Special Issue Peptide Nucleic Acids: Applications in Biomedical Sciences)
This review describes the application of peptide nucleic acids (PNAs) as clamps that prevent nucleic acid amplification of wild-type DNA so that DNA with mutations may be observed. These methods are useful to detect single-nucleotide polymorphisms (SNPs) in cases where there is a small amount of mutated DNA relative to the amount of normal (unmutated/wild-type) DNA. Detecting SNPs arising from mutated DNA can be useful to diagnose various genetic diseases, and is especially important in cancer diagnostics for early detection, proper diagnosis, and monitoring of disease progression. Most examples use PNA clamps to inhibit PCR amplification of wild-type DNA to identify the presence of mutated DNA associated with various types of cancer. View Full-Text
Keywords: peptide nucleic acids (PNAs); single-nucleotide polymorphism (SNP); polymerase chain reaction (PCR); cancer. peptide nucleic acids (PNAs); single-nucleotide polymorphism (SNP); polymerase chain reaction (PCR); cancer.
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Fouz, M.F.; Appella, D.H. PNA Clamping in Nucleic Acid Amplification Protocols to Detect Single Nucleotide Mutations Related to Cancer. Molecules 2020, 25, 786.

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