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l-DNA-Based Catalytic Hairpin Assembly Circuit

Department of Chemistry, Texas A&M University, College Station, TX, 77843, USA
Author to whom correspondence should be addressed.
Academic Editor: Eylon Yavin
Molecules 2020, 25(4), 947;
Received: 4 February 2020 / Revised: 18 February 2020 / Accepted: 19 February 2020 / Published: 20 February 2020
(This article belongs to the Special Issue Peptide Nucleic Acids: Applications in Biomedical Sciences)
Isothermal, enzyme-free amplification methods based on DNA strand-displacement reactions show great promise for applications in biosensing and disease diagnostics but operating such systems within biological environments remains extremely challenging due to the susceptibility of DNA to nuclease degradation. Here, we report a catalytic hairpin assembly (CHA) circuit constructed from nuclease-resistant l-DNA that is capable of unimpeded signal amplification in the presence of 10% fetal bovine serum (FBS). The superior biostability of the l-DNA CHA circuit relative to its native d-DNA counterpart was clearly demonstrated through a direct comparison of the two systems (d versus l) under various conditions. Importantly, we show that the l-CHA circuit can be sequence-specifically interfaced with an endogenous d-nucleic acid biomarker via an achiral peptide nucleic acid (PNA) intermediary, enabling catalytic detection of the target in FBS. Overall, this work establishes a blueprint for the detection of low-abundance nucleic acids in harsh biological environments and provides further impetus for the construction of DNA nanotechnology using l-oligonucleotides. View Full-Text
Keywords: catalytic hairpin assembly (CHA); strand-displacement reaction; peptide nucleic acid; l-DNA; microRNA catalytic hairpin assembly (CHA); strand-displacement reaction; peptide nucleic acid; l-DNA; microRNA
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MDPI and ACS Style

Kabza, A.M.; Sczepanski, J.T. l-DNA-Based Catalytic Hairpin Assembly Circuit. Molecules 2020, 25, 947.

AMA Style

Kabza AM, Sczepanski JT. l-DNA-Based Catalytic Hairpin Assembly Circuit. Molecules. 2020; 25(4):947.

Chicago/Turabian Style

Kabza, Adam M., and Jonathan T. Sczepanski 2020. "l-DNA-Based Catalytic Hairpin Assembly Circuit" Molecules 25, no. 4: 947.

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