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Article

The [DPPH●/DPPH-H]-HPLC-DAD Method on Tracking the Antioxidant Activity of Pure Antioxidants and Goutweed (Aegopodium podagraria L.) Hydroalcoholic Extracts

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Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a (Collegium Pharmaceuticum), 20-093 Lublin, Poland
2
Faculty of Medicine, Medical University of Lublin, Aleje Racławickie 1, 20-059 Lublin, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Luisa Tesoriere
Molecules 2020, 25(24), 6005; https://doi.org/10.3390/molecules25246005
Received: 3 December 2020 / Revised: 15 December 2020 / Accepted: 16 December 2020 / Published: 18 December 2020
(This article belongs to the Section Photochemistry)
The 2,2-diphenyl-1-picrylhydrazyl (DPPH)-reverse phase (RP)-HPLC-diode array detector (DAD) method was tested on standard antioxidants (AOs), i.e., reduced glutathione (GSH), ascorbic acid (vitamin C), and alcoholic extracts of A. podagraria L. An elaborated HPLC procedure enabled the simultaneous measurement of the redox couple DPPH-R (2,2-diphenyl-1-picrylhydrazyl radical)/DPPH-H (2,2-diphenyl-1-picrylhydrazine). Both forms were fully separated (Rs = 2.30, α = 1.65) on a Zorbax Eclipse XDB-C18 column eluted with methanol–water (80:20, v/v) and detected at different wavelengths in the range of 200–600 nm. The absorbance increases of the DPPH-H as well as the DPPH-R peak inhibition were measured at different wavelengths in visible and UV ranges. The chromatographic method was optimized, according to reaction time (slow, fast kinetics), the linearity range of DPPH radical depending on the detection conditions as well as the kind of the investigated antioxidants (reference chemicals and the ground elder prepared from fresh and dry plants). The scavenging capacity was expressed by the use of percentage of peak inhibition and the IC50 parameters. The evaluated extracts displayed antioxidant activity, higher than 20% inhibition against 350 µM DPPH free radical. The results show that extract prepared from dry plants in the ultrasonic bath exhibits the highest antioxidant potential (IC50 = 64.74 ± 0.22 µL/mL). View Full-Text
Keywords: Aegopodium podagraria L.; goutweed; antioxidant activity; DPPH; RP-HPLC Aegopodium podagraria L.; goutweed; antioxidant activity; DPPH; RP-HPLC
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MDPI and ACS Style

Flieger, J.; Flieger, M. The [DPPH●/DPPH-H]-HPLC-DAD Method on Tracking the Antioxidant Activity of Pure Antioxidants and Goutweed (Aegopodium podagraria L.) Hydroalcoholic Extracts. Molecules 2020, 25, 6005. https://doi.org/10.3390/molecules25246005

AMA Style

Flieger J, Flieger M. The [DPPH●/DPPH-H]-HPLC-DAD Method on Tracking the Antioxidant Activity of Pure Antioxidants and Goutweed (Aegopodium podagraria L.) Hydroalcoholic Extracts. Molecules. 2020; 25(24):6005. https://doi.org/10.3390/molecules25246005

Chicago/Turabian Style

Flieger, Jolanta, and Michał Flieger. 2020. "The [DPPH●/DPPH-H]-HPLC-DAD Method on Tracking the Antioxidant Activity of Pure Antioxidants and Goutweed (Aegopodium podagraria L.) Hydroalcoholic Extracts" Molecules 25, no. 24: 6005. https://doi.org/10.3390/molecules25246005

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