Next Article in Journal
Assessment of Tissue Distribution and Metabolism of MP1, a Novel Pyrrolomycin, in Mice Using a Validated LC-MS/MS Method
Previous Article in Journal
In Vivo Targeted Metabolomic Profiling of Prostanit, a Novel Anti-PAD NO-Donating Alprostadil-Based Drug
Previous Article in Special Issue
Surface Plasmon Resonances in Sierpinski-Like Photonic Crystal Fibers: Polarization Filters and Sensing Applications
Open AccessFeature PaperArticle

Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy

1
Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland
2
Institute of Biomedicine, Faculty of Medicine, University of Turku, 20520 Turku, Finland
3
Cancer Research Laboratory FICAN West, Institute of Biomedicine, University of Turku, 20520 Turku, Finland
4
Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland
5
Adámas Nanotechnologies, Inc., 8100 Brownleigh Drive, Suite 120, Raleigh, NC 27617, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Miguel Levy and Dolendra Karki
Molecules 2020, 25(24), 5897; https://doi.org/10.3390/molecules25245897
Received: 11 November 2020 / Revised: 7 December 2020 / Accepted: 12 December 2020 / Published: 13 December 2020
(This article belongs to the Special Issue Advances in Photonic Materials)
Correlative light and electron microscopy (CLEM) is revolutionizing how cell samples are studied. CLEM provides a combination of the molecular and ultrastructural information about a cell. For the execution of CLEM experiments, multimodal fiducial landmarks are applied to precisely overlay light and electron microscopy images. Currently applied fiducials such as quantum dots and organic dye-labeled nanoparticles can be irreversibly quenched by electron beam exposure during electron microscopy. Generally, the sample is therefore investigated with a light microscope first and later with an electron microscope. A versatile fiducial landmark should offer to switch back from electron microscopy to light microscopy while preserving its fluorescent properties. Here, we evaluated green fluorescent and electron dense nanodiamonds for the execution of CLEM experiments and precisely correlated light microscopy and electron microscopy images. We demonstrated that green color emitting fluorescent nanodiamonds withstand electron beam exposure, harsh chemical treatments, heavy metal straining, and, importantly, their fluorescent properties remained intact for light microscopy. View Full-Text
Keywords: CLEM; nanodiamonds; single-cell microscopy; fiducial; dual probes CLEM; nanodiamonds; single-cell microscopy; fiducial; dual probes
Show Figures

Graphical abstract

MDPI and ACS Style

Prabhakar, N.; Peurla, M.; Shenderova, O.; Rosenholm, J.M. Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy. Molecules 2020, 25, 5897. https://doi.org/10.3390/molecules25245897

AMA Style

Prabhakar N, Peurla M, Shenderova O, Rosenholm JM. Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy. Molecules. 2020; 25(24):5897. https://doi.org/10.3390/molecules25245897

Chicago/Turabian Style

Prabhakar, Neeraj; Peurla, Markus; Shenderova, Olga; Rosenholm, Jessica M. 2020. "Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy" Molecules 25, no. 24: 5897. https://doi.org/10.3390/molecules25245897

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop