Protective Effects of Traditional Polyherbs on Cisplatin-Induced Acute Kidney Injury Cell Model by Inhibiting Oxidative Stress and MAPK Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Free Radical Scavenging Activities of Traditional Polyherbs
2.2. Effects on Cell Viabilities in Cisplatin-Induced Nephrotoxicity
2.3. Effects on ROS Production and Antioxidant Activities
2.4. Effects on Apoptotic Changes
2.5. Effects on Cell Proliferation
2.6. Effects on Expression of Caspase-3 and MAPK Signaling Proteins
3. Discussion
4. Materials and Methods
4.1. Preparation of Traditional Polyherbs
4.2. Free Radical Scavenging Activity
4.3. Cell Culture
4.4. Cisplatin-Induced Acute Kidney Cell Model and Treatments
4.5. Cell Viability Assay
4.6. Assessment of ROS Levels
4.7. Assessment of Activities of Antioxidant Enzymes
4.8. Flow Cytometric Analysis
4.9. Immunoblotting
4.10. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ingredients | |
---|---|
IJ | Artemisiae Capillaris Herba 2 g, Gardenia Fruit 1 g, Rhubarb 0.67 g |
Uro | Akebiae Caulis 416.7 mg, Alisma Rhizome 250 mg, Angelica Gigas Root 416.7 mg, Cinnamon Bark 16.7 mg, Ephedra Herb 16.7 mg, Forsythia Fruit 16.7 mg, Gardenia Fruit 125 mg, Gentian Root 125 mg, Glycyrrhiza 125 mg, Plantago Seed 250 mg, Rhubarb 16.7 mg, Scutellaria Root 250 mg, Raw Ginger 16.7 mg, Rehmannia Root 416.7 mg |
US | Achyranthes Root 3.0 mg, Alisma Rhizome 3.0 mg, Cinnamon Bark 1 g, Cornus Fruit 3.0 mg, Dioscorea Rhizome 3.0 mg, Hoelen 3 g, Moutan Root Bark 3.0 mg, Psyllium Husk 3 g, Pulvis Aconiti Tuberis Purificatum 1.0 mg, Rehmannia Root 5.0 mg |
YJ | Alisma Rhizome 240 mg, Cornus Fruit 320 mg, Dioscorea Rhizome 320 mg, Hoelen 240 mg, Moutan Root Bark 240 mg, Steamed Rehmannia Root 640 mg |
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Dachuri, V.; Song, P.H.; Kim, Y.W.; Ku, S.-K.; Song, C.-H. Protective Effects of Traditional Polyherbs on Cisplatin-Induced Acute Kidney Injury Cell Model by Inhibiting Oxidative Stress and MAPK Signaling Pathway. Molecules 2020, 25, 5641. https://doi.org/10.3390/molecules25235641
Dachuri V, Song PH, Kim YW, Ku S-K, Song C-H. Protective Effects of Traditional Polyherbs on Cisplatin-Induced Acute Kidney Injury Cell Model by Inhibiting Oxidative Stress and MAPK Signaling Pathway. Molecules. 2020; 25(23):5641. https://doi.org/10.3390/molecules25235641
Chicago/Turabian StyleDachuri, VinayKumar, Phil Hyun Song, Young Woo Kim, Sae-Kwang Ku, and Chang-Hyun Song. 2020. "Protective Effects of Traditional Polyherbs on Cisplatin-Induced Acute Kidney Injury Cell Model by Inhibiting Oxidative Stress and MAPK Signaling Pathway" Molecules 25, no. 23: 5641. https://doi.org/10.3390/molecules25235641