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Open AccessArticle

Synergistic Effect of Propidium Iodide and Small Molecule Antibiotics with the Antimicrobial Peptide Dendrimer G3KL against Gram-Negative Bacteria

1
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland
2
Department of Microbiology and Molecular Medicine, University of Geneva, 1211 Geneva, Switzerland
3
Service of Infectious Diseases, Geneva University Hospitals, 1211 Geneva, Switzerland
*
Author to whom correspondence should be addressed.
Academic Editor: Paul Robert Hansen
Molecules 2020, 25(23), 5643; https://doi.org/10.3390/molecules25235643
Received: 15 October 2020 / Revised: 24 November 2020 / Accepted: 26 November 2020 / Published: 30 November 2020
There is an urgent need to develop new antibiotics against multidrug-resistant bacteria. Many antimicrobial peptides (AMPs) are active against such bacteria and often act by destabilizing membranes, a mechanism that can also be used to permeabilize bacteria to other antibiotics, resulting in synergistic effects. We recently showed that G3KL, an AMP with a multibranched dendritic topology of the peptide chain, permeabilizes the inner and outer membranes of Gram-negative bacteria including multidrug-resistant strains, leading to efficient bacterial killing. Here, we show that permeabilization of the outer and inner membranes of Pseudomonas aeruginosa by G3KL, initially detected using the DNA-binding fluorogenic dye propidium iodide (PI), also leads to a synergistic effect between G3KL and PI in this bacterium. We also identify a synergistic effect between G3KL and six different antibiotics against the Gram-negative Klebsiella pneumoniae, against which G3KL is inactive. View Full-Text
Keywords: antimicrobial peptides; dendrimers; membrane permeabilization; antibiotics; synergy antimicrobial peptides; dendrimers; membrane permeabilization; antibiotics; synergy
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MDPI and ACS Style

Gan, B.-H.; Cai, X.; Javor, S.; Köhler, T.; Reymond, J.-L. Synergistic Effect of Propidium Iodide and Small Molecule Antibiotics with the Antimicrobial Peptide Dendrimer G3KL against Gram-Negative Bacteria. Molecules 2020, 25, 5643.

AMA Style

Gan B-H, Cai X, Javor S, Köhler T, Reymond J-L. Synergistic Effect of Propidium Iodide and Small Molecule Antibiotics with the Antimicrobial Peptide Dendrimer G3KL against Gram-Negative Bacteria. Molecules. 2020; 25(23):5643.

Chicago/Turabian Style

Gan, Bee-Ha; Cai, Xingguang; Javor, Sacha; Köhler, Thilo; Reymond, Jean-Louis. 2020. "Synergistic Effect of Propidium Iodide and Small Molecule Antibiotics with the Antimicrobial Peptide Dendrimer G3KL against Gram-Negative Bacteria" Molecules 25, no. 23: 5643.

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