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Significance of Artemisia Vulgaris L. (Common Mugwort) in the History of Medicine and Its Possible Contemporary Applications Substantiated by Phytochemical and Pharmacological Studies

Chair and Department of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, Poland
Family Medicine Clinic, Medizinisches Versorgungszentrum (MVZ) Burgbernheim GmbH, Gruene Baumgasse 2, 91593 Burgbernheim, Germany
Museum of Pharmacy, Medical College, Jagiellonian University, Floriańska 25, 31-019 Kraków, Poland
Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, Gronostajowa 9, 30-387 Kraków, Poland
Authors to whom correspondence should be addressed.
Molecules 2020, 25(19), 4415;
Submission received: 3 September 2020 / Revised: 21 September 2020 / Accepted: 23 September 2020 / Published: 25 September 2020
(This article belongs to the Special Issue Biological Activities of Natural Products)


Artemisia vulgaris L. (common mugwort) is a species with great importance in the history of medicine and was called the “mother of herbs” in the Middle Ages. It is a common herbaceous plant that exhibits high morphological and phytochemical variability depending on the location where it occurs. This species is well known almost all over the world. Its herb—Artemisiae vulgaris herba—is used as a raw material due to the presence of essential oil, flavonoids, and sesquiterpenoids lactones and their associated biological activities. The European Pharmacopoeia has listed this species as a potential homeopathic raw material. Moreover, this species has been used in traditional Chinese, Hindu, and European medicine to regulate the functioning of the gastrointestinal system and treat various gynecological diseases. The general aim of this review was to analyze the progress of phytochemical and pharmacological as well as professional scientific studies focusing on A. vulgaris. Thus far, numerous authors have confirmed the beneficial properties of A. vulgaris herb extracts, including their antioxidant, hepatoprotective, antispasmolytic, antinociceptive, estrogenic, cytotoxic, antibacterial, and antifungal effects. In addition, several works have reviewed the use of this species in the production of cosmetics and its role as a valuable spice in the food industry. Furthermore, biotechnological micropropagation of A. vulgaris has been analyzed.

1. Introduction

In 2015, the awarding of the Nobel Prize in Medicine for the discovery of artemisinin, a compound of plant origin found in Artemisia annua (annual mugwort), inspired the researchers to study the phytochemical and pharmacological properties of other species of the genus Artemisia. Recently, this species has been taken under consideration to be active toward the virus SARS-CoV-2 and disease COVID-19 [1,2].
Artemisia vulgaris L. (common mugwort) is one of the best-known species of this genus, which has a widespread distribution in the natural habitats worldwide (Europe, Asia, North and South America, and Africa). For many centuries, this species has been mainly used for treating gynecological ailments and gastrointestinal diseases [3,4,5,6,7]. Recently, researches have proved that this species exhibits antioxidant, hypolipidemic, hepatoprotective, antispasmolytic, analgesic, estrogenic, cytotoxic, antibacterial, antifungal, hypotensive, and broncholytic effects [8,9,10,11,12,13,14,15,16,17].
The different applications of this plant species have been possible due to its rich chemical composition, which especially includes essential oils, flavonoids, sesquiterpene lactones, phenolic acids, coumarins, and other groups of metabolites.
The presence of essential oil in A. vulgaris contributes to the significance of this species as a culinary spice in the food industry in various regions of the world. Currently, this species is also increasingly used in the production of cosmetics in Europe as well as in Asia and North America [14,18,19,20].
The latest review on A. vulgaris presented by Brazilian–Iran and Malaysian teams highlighted the value of this plant species from the South American–Asian point of view [21].
The present review provides classical information about the importance of A. vulgaris in therapeutics and the food industry from the European point of view and additionally discusses the possible new applications of this plant species in phytotherapy as a hepatoprotective, broncholytic, anthelmintic, and cytotoxic agent and in the cosmetics industry as a raw material in Europe, East Asia (especially in Korea), and North America.

2. Distribution and Taxonomic Consideration of Artemisia

The Artemisia genus belongs to subtribe Artemisiinae of tribe Anthemidae from Asteraceae family and comprises more than 500 species. These species are distributed all over the world, especially in the moderate climate zones of Europe, East Asia, Americas, North Africa, and Australia [22,23,24].
The plants of this genus are annual, biennial, and perennial herbs or small shrubs and halfshrubs. From the chemotaxonomic point of view, these plants are rich in essential oils and bitter substances (particularly sesquiterpenoid lactones). They are also a good source of flavonoids, coumarins, and phenolic acids [25].
Several representatives of this genus are found in Asia—about 150 species in China and about 174 in the ex-USSR. Many species are characteristic of Japan and Iran flora, (about 50 and 35, respectively). The number of Artemisia sp. occurring in Europe is estimated at 57 [26].
In Poland, 10 different species, including A. vulgaris and Artemisia absinthium, are found in the natural habitats [27]. Some of them (e.g., Artemisia petrosa Baung and Artemisia pontica L.) are distributed only on small areas in the country and are on the list of endangered plant species [27,28].
One of the most popular species worldwide is A. vulgaris (common mugwort). It is known by various synonymous Latin names, presented in Table 1. This can be attributed to the broad distribution of this species on all continents and the differences in its chemical and genetic composition, which is characteristic of the plants of different origins.
According to the data available on the official website “The Plant List” (created by Global Strategy for Plant Conservation and World Flora Online), A. vulgaris has as many as 107 synonymous Latin names, including Absinthium spicatum (Wulfen ex Jacq.) Baumg [3,29], Artemisia affinis Hassk. [3,29], Artemisia opulenta Pamp. [3,29,30], Artemisia vulgaris subsp. vulgaris [31], and Artemisia vulgaris var. indica (Willd.) Hassk. [3,29,31] (Table 1). In addition, again due to its widespread dissemination, this species is known by various foreign-language names, including Carline Thistle, Chiu Ts’Ao, common mugwort, Chrysanthemum weed, Cingulum Sancti Johannis, common wormwood (English), Ajenjo, altamis, altamisa (Spanish), altamiza, amarella (Italian), armoise, Armoise citronnelle (French), beiai (Chinese), Beifußkraut (German), Nagadamani (Ayurvedic), moxa (Japan) and Polynesian snare (Russian) [3,4,29,30,31].

3. Genetic Issues

Artemisia (Asteraceae, Anthemideae, Artemisiinae) is the largest genus of the tribe Anthemideae. It is also one of the broadest genera of the family Asteraceae, which comprises more than 500 species [32,33]. The genus exhibits high morphological and phytochemical variability and ecological plasticity, with species occurring from sea level to huge mountains and from arid zones to wetlands [22]. At present, the following five main groups are considered at the subgeneric level, mostly based on the floral characteristics: Artemisia, Absinthium (Mill.) Less., Dracunculus (Besser) Rydb., Seriphidium Besser, and Tridentatae (Rydb.) McArthur [33,34].
According to Reference [32], Artemisia has two basic chromosome numbers, the largely predominating x = 9 and the less extended x = 8, with the ploidy levels ranging from diploid to dodecaploid in the species with x = 9 chromosomes and from diploid to hexaploid in those with x = 8 chromosomes. The chromosome number x = 9 is the most common not only in the genus Artemisia but also in the tribe Anthemideae and the family Asteraceae [33]. The evolutionary pathway determining the chromosome number in Artemisia is affected by the successive or simultaneous effects of polyploidy (both autopolyploidy and allopolyploidy) and dysploidy [35]. The existence of two basic chromosome numbers indicates that Artemisia is a dysploid genus. Dysploidy is seen in Artemisia, Absinthium, and Dracunculus, which are the three main groups of the genus, while Seriphidium and Tridentatae display only one basic chromosome number, x = 9 [32].
According to Reference [34], dysploid–polyploid complexes have played a major role in the karyological evolution of Artemisia. Dysploidy refers to change in the chromosomal base number through the rearrangement of chromatin and the loss or gain of a centromere without any change in the amount of chromatin in the karyotype. A high percentage of Artemisia species are polyploids, and the reported cytotypes cytogenetically differ in their external morphology, anatomy, fertility, and phytochemical characteristics [35]. Among the species, A. vulgaris is quite diverse showing several cytotypes, of which 2n = 2x = 18 is the most common [36].
In most cases, differences in the 2C values of about sevenfold have been detected between the species. These are directly related to the polyploidization events [33], which reflects the high genetic plasticity of the genus Artemisia. This phenomenon is also seen in all the major groups of this genus. In diploids, the chromosome number is most often 2n = 18 or 16, but ploidy levels of up to 16× have been reported. The highest chromosome number known is 144, and aneuploidy is frequent as well [37].
In Europe and North America, as well as in Altai and Russia, the most commonly observed ploidy of A. vulgaris was 2n = 16 (x = 8) [38], while specimens from the high Himalayas, which underwent a period of glaciation, were found to be mainly diploids having 2n = 18 chromosomes [39]. At different elevations in this same region, examples of tetraploid (2n = 36) and hexaploid (2n = 54) were also found [39]. However, biotypes from other regions of the world have been reported with chromosome numbers of 2n = 16, 18, 24, or 36 [7].
Artemisia is one of the 530 genera of the Asteraceae family in which polyploidy was detected. It is a good example of polyploid series and ploidy-level variation, with both autopolyploids and allopolyploids reported [35]. According to Reference [33], approximately 43.5% of Artemisia species are exclusively diploid and 29.7% are exclusively polyploid, while around 26.8% of species are both diploid and polyploid. The high percentage of polyploids and the ability of the species to colonize different ecosystems, as well as their adaptation to different habitat conditions, may be the main features that favored the successful expansion of this genus across the landscape.
The chromosomes of Artemisia are rather small (2–8 μm). Their karyotypes tend to be symmetric, considering both the interchromosomal and intrachromosomal asymmetry [33,39]. The use of Giemsa C-banding and chromomycin and bisbenzimide fluorochrome banding allowed using the heterochromatic chromosome segment banding patterns for systematic study and characterizing Artemisia as a genus having few intercalary C-bands in the chromosomes [33,39].
The distribution of heterochromatin varies between taxa. The population of A. vulgaris is characterized by centromeric or pericentromeric heterochromatin. The cytogenetic data, especially those related to the heterochromatin, may be helpful in understanding the role played by polyploidy in the evolution of the genus Artemisia. The diploid and tetraploid taxa were identified to have different chromosome structures: the tetraploid species studied showed two types of heterochromatin (AT- and CG-rich), whereas the diploids showed only the GC-rich regions [40].
The molecular phylogenetic data basically agree with the classical taxonomy, but some questions remain unsolved. The taxonomic classification of Artemisia sp. has been controversial due to the following reasons: insufficient diagnostic features, highly variable morphological traits, the possibility of natural hybridization among taxa, polyploidy, and nomenclatural legacy [35].
According to Reference [41], sequencing of nuclear and organelle genome regions, such as the external and internal transcribed spacers of nuclear ribosomal DNA and the intergenic spacers between the genes of chloroplast genome (plastome), has enabled the researchers to perform molecular phylogenetic analyses of Artemisia. They reported that the complete plastome sequences of this genus are sufficiently polymorphic to be used as superbarcodes for its species. This in turn will facilitate the development of new molecular markers and allow studying the phylogenomic relationships of Artemisia species in the family Asteraceae.

4. Botanical Characteristics

The species A. vulgaris shows high morphological variability depending on the place of occurrence [30]. Comparative studies conducted between different populations revealed variability in the branching (presence or absence and extent), leaf shape, and root diameter of the plant [7]. The high variability of A. vulgaris has been confirmed by the study conducted by Barney and DiTommaso on two populations collected from different, geographically isolated areas of Ithaca (USA). The authors found that plants from one population had densely hairy stems and light green leaves, each with a few deep notches, whereas those collected from another part of the city had almost smooth stems and dark green leaves with many deep notches [42].
Artemisia vulgaris is a herbaceous plant which grows up to a length of 2.5 m and has a width of 75 cm. It is characterized by an intense aroma that is readily released when the leaves are crushed [30,43], and a spicy taste [44]. The plant has a thick main root and many small, fibrous lateral roots. The roots take on a light-brown color and measure up to 1 cm. They remain in the upper layer of the soil, at a depth of 7–18 cm, forming a vast, underground network [7,30]. The stems of the plant are slightly wavy, straight, or branched, having a brown color at the lower end, and become woody with age, appearing green further up and purple at the top. Some of the stems are also hairy [7,45]. The leaves are 5–10 cm long. They are set densely, and alternately, primarily in the upper parts of the stem. The lower leaves with short petioles are divided into segments and take on a feathery shape, while the middle and upper ones are smaller and single or double pinnate. The dorsal side of the leaves has a dark green color, while the ventral side is whitish and tomentose [7,46]. Small, almost bare, yellowish or brown-red flowers are embedded in small baskets that form heavily branched panicles with numerous lanceolate bracts at the top of the shoots [46]. One basket may contain around 15–30 flowers with numerous stamens [7]. Studies conducted in the eastern part of the USA showed that the inflorescences contain 52% of ligulate flowers and 48% of tubular flowers [7], of which 25–50% are female [47].
After the flowering period, which lasts from July to September in Europe, A. vulgaris develops fruit—called achenes. The seeds of these fruits are brown, weighing 0.12–0.14 mg, and have a narrow base. They are oblong and ridged, covered with fine hairs on the apex [7,48].
The species reproduces from seeds, which can produce up to 200,000 per year depending on the habitat [49], but so far this has been observed only in the native places of occurrence, namely in Asia and Europe. Some biotypes of this plant do not produce reproductive seeds [5]. The seeds of A. vulgaris spread through wind, beetles, and flies [47]. The plant usually reproduces vegetatively with the help of its roots, which can survive in the ground during winter. In the northern USA, pieces of roots are often moved long distances by local floods [7].
The leaves and roots of A. vulgaris exhibit strong allelopathic properties [42,50]. Although this has been confirmed in fresh leaves, it was not possible to isolate the specific compound that is responsible for these properties. It is suspected that these are contributed by a mixture of compounds from the monoterpenoid group [42].

5. Natural Habitats and Cultivation

Most sources indicate that A. vulgaris originated from Europe and Asia [7,48], and from there, this species was brought to North America, probably at the beginning of the 16th century [7].
At present, the plant is abundantly seen in many regions of the world, ranging from the Himalayas in Asia, through Europe, to the warm areas of North America [22,30]. The only continent where A. vulgaris does not occur is Antarctica [7].
This species is widely considered a weed [7,31]. It can be found in many habitats, for example, on roadsides, along rivers, or in abandoned mines, thickets, tree nurseries, and arable or other fields, where it interferes with the growth of different plants [3,5,7].
Individual populations of A. vulgaris are well adapted to live in a wide range of pH and various soil types, including sandy and loamy. Due to its extensive root system, this plant can quickly occupy large areas [7]. Controlling the spread of A. vulgaris is very difficult because only a few effective ways can limit its growth [5,7].
As a species with low requirements, A. vulgaris can easily colonize successive sites and displace native species. Thus, it can easily disturb the local ecosystems [7,30].
This species is cultivated on an industrial scale in Italy, France, Brazil, and Japan, as well as in the mountainous regions of India and Sri Lanka [3]. It is also possible to grow this plant in home gardens or can be obtained from natural habitats.
The aerial parts of the plant die each year [46], and hence, they are harvested at the beginning of flowering. The parts are obtained by cutting the tops of shoots, while the woody stems are omitted. Then, these are dried in airy drying sheds under natural conditions [18]. After drying, the herb has a spicy, bitter taste and a balsamic aroma.
The appropriate time to harvest the roots is at the beginning of winter [18]. Drying is carried out at 40 °C in drying sheds. The roots that are properly harvested and dried are brittle and have a light-brown color.

6. Status of the Plant in Official Phytotherapy in Europe

The aerial parts of A. vulgarisArtemisiae vulgaris herba—are usually used as a pharmaceutical raw material [31], while the roots—Artemisiae vulgaris radix—harvested in early winter, are less frequently used [18]. Both these raw materials do not have their monographs in the latest editions of pharmacopoeias; however, A. vulgaris herba had a monograph in the German Pharmacopoeia published in 1988 [51]. In the latest European Pharmacopoeia [52] and in the French Pharmacopoeia [53], the species is only mentioned as a homeopathic raw material. Although the plant has a monograph published by the EFSA [18], no opinions regarding it have been issued by the European Scientific Cooperative on Phytotherapy (ESCOP) or European Medicines Agency (EMA).

7. Phytochemical Characteristics

Various groups of compounds can be distinguished in A. vulgaris, including sesquiterpenoid lactones, flavonoids, coumarins, phenolic acids, sterols, polyacetylenes, carotenoids, vitamins, and cyanogenic glycosides. Essential oil is another important substance found in the plant. Due to the high intraspecific diversity and discrepancies in the chemical composition of the plant determined by using various test methods, it is difficult to indicate a distinct phytochemical profile for A. vulgaris [15]. A characteristic feature of this species is the presence of sesquiterpenoid lactones, including psilostachyin, psilostachyin C, and vulgarin (Figure 1), and also artemisinin was confirmed. In addition, the presence of flavonoids—derivatives of kaempferol and quercetin, and coumarin compounds, such as esculin, umbelliferone, and scopoletin, is a distinguishing attribute of the plant (Table 2).
The presence of essential oil is an interesting characteristic of A. vulgaris. A major part of the oil extracted from the aerial parts is constituted by monoterpenoids (72%) and sesquiterpenoids (26%). Among the volatile compounds, the following are the most commonly identified: 1,8-cineole, sabinene, camphor, camphene, caryophyllene oxide, α-thujone, and β-thujone (Table 3 and Figure 2).
Many experiments have revealed the significant differences in the quality and quantity of the components of the essential oils obtained from the A. vulgaris plants grown in different parts of the world (Table 4).
Although the root of this species is sometimes used as raw material, only a few publications have described its chemical composition. A group of scientists from the University of Niš in Niš (Serbia and Montenegro) analyzed the differences in the phytochemical profile of the essential oil obtained from its green parts and root. The plant materials used in the study were collected during the flowering period. They were dried and then subjected to steam distillation in a Clevenger apparatus. The isolated compounds were identified using gas chromatography, gas chromatography–mass spectrometry, and 13C nuclear magnetic resonance. The main compounds in the oil extracted from the root were neryl 2-methylbutanoate (13.2%), β-eudesmol (10.4%), and bornyl 3-methylbutanoate (8.45%). However, none of these substances was found in the oil obtained from the aerial parts. The underground part of the plant also contained some rare compounds belonging to, inter alia, the group of tricyclic sesquiterpenoids, such as (−)-α-isocomene, (−)-isocomene, silphin-1-ene, presilphiperfol-7-ene, and modhephene [16] (Figure 3).
In the essential oil extracted from the aerial parts of A. vulgaris, no tricyclic sesquiterpenoids were detected. The dominant compounds that were identified in the study were 1,8-cineole (28.9%), sabinene (13.7%), β-thujone (13.5%), and β-caryophyllene oxide (6.5%), but these were absent in the underground parts of the plant. Based on these findings, the authors concluded that the differences in the chemical composition of the oils extracted from the root and aerial parts might indicate the coexistence of different biogenetic pathways in the plant species [16].

8. History of Medicinal Use

Due to its widespread occurrence, A. vulgaris was well known in ancient Egypt, Greece, and Rome. According to ancient belief, its name is derived from the name of the Greek goddess Artemis, who is the patron of pregnant women and newly delivered mothers. Because of its beneficial effects on menstruation- and pregnancy-related ailments, A. vulgaris had great importance in the religious rites devoted to the goddesses Isis, Artemis, and Diana. The healing properties of this species were described in medical works in as early as the 1st century A.D. by Dioscorides in “Materia medica” [81], by Pliny the Elder in “Naturalis Historia” [82], and by Galen in “De simplicium medicamentorum facultatibus” [83]. Furthermore, the mugwort plant was credited with warming and drying effects, and therefore, it was also recommended for the treatment of urological diseases, such as dysuria or nephrolithiasis. In medieval medicine, A. vulgaris, called “mater herbarum” (the mother of herbs), was used externally for treating wounds, against gout, and to remove leg fatigue, as well as in an attempt to treat fever [84]. In addition, the plant gained popularity as a remedy for gastrointestinal ailments “resulting from cold” [85], including stomach pain, diarrhea, and intestinal colic. It was believed to be effective against jaundice when served with wine and against goiter when applied as a poultice [86]. During the Renaissance, thanks to, inter alia, the invention of printing by J. Gutenberg, the holistic medicine flourished in Europe, lasting until the 18th century. At that time, in addition to women’s diseases, the therapeutic spectrum of A. vulgaris was expanded to include spleen and liver diseases. Additional recommendations appeared “for enlarged and distended spleen” [87], “against clogged liver” [88], and “for cold lower abdomen” [89]. During the development of modern medicine in the 19th century, epilepsy and neurosis were included among the indications for treatment with mugwort [90]. In the 20th century, the scientific and laboratory analyses of the composition of A. vulgaris led to the declaration that due to its high allergic potential the herb is not suitable for medicinal use and can only be used in culinary applications, and that its place in the household is the kitchen, not the medicine cabinet [63].

9. Applications in Traditional Medicine Worldwide

In Asian medicine, A. vulgaris is often used for alleviating gastrointestinal discomfort and treating gynecological diseases [5,6].
In China, A. vulgaris is traditionally used to treat cholera and leprosy. Other indications include hemorrhagic conditions, such as the presence of blood in sputum, stool, and vomit, and nosebleeds [18]. The essential oil of this species is used as a popular herbal medicine called “Ai Hao,” and is prescribed for curing ulcers and diarrhea [7].
The Traditional Chinese Medicine (TCM) also recommends thermopuncture consisting in burning “moxa”—dried and powdered leaves of A. vulgaris—directly on or close to the skin, or inhaling the resulting smoke, which allows the migration of “chi” energy [91]. Indications for this treatment include tumors, although its effectiveness has not been confirmed so far [92]. In 2009, Reference [93] investigated the harmfulness of compounds formed during the combustion of this plant species. The authors tested the smoke emitted by moxa for the presence of compounds commonly found in cigarettes, such as tar, nicotine, carbon monoxide, polycyclic aromatic hydrocarbons, ammonia, hydrogen cyanide, polyaromatic amines, volatile organic compounds, semi-volatile compounds, phenols, and carbonyls (e.g., formaldehyde and acetone), all of which are associated with the highest risk of cancer. Their results showed that even at concentrations higher by an order of magnitude than that achieved with the inhalation of normal smoke, only a few of the analytes exceeded the permissible standards. These included aromatic amines such as 1-aminonaphthalene, 2-aminonaphthalene, 3-aminobiphenyl, and 4-aminobiphenyl. Therefore, the authors concluded that burning moxa should not pose a health risk. Evidence of exceeded limits appeared only after the extrapolation of results for long-term use, which probably did not reflect the actual concentration of the tested compounds [93]. In TCM, A. vulgaris is most often used in thermopuncture in the form of cotton buds impregnated with the plant extract, or as cigarettes filled with dried leaves and used to cauterize the skin.
The species is also considered a substitute for cannabis. When being smoked, it exhibits mild intoxicating properties and strong relaxing properties [18]. In traditional Hindu medicine (Unani), many preparations based on A. vulgaris are used. For instance, “Arq-e-Afsanteen” is a preparation recommended for liver inflammation and obstruction. “Dava-ul-Luk” is used for treating enlarged liver or spleen and nephrolithiasis. The medication “Qurs-e-Gul” is indicated in chronic fever as a liver tonic, while decoctions from the plant are taken during dysmenorrhea [94]. In addition, liquid extract or dried greens at a single dose of 0.5–2 g is used in Ayurvedic medicine [3].
The species is also well known in the traditional medicine of South America, and often used against fever, malaria, and gastric disorders [95,96,97].
In European folk medicine, after oral administration, the A. vulgaris herb stimulates the secretion of gastric juice. Hence, it is used against gastrointestinal catarrh, insufficient production of bile and digestive juices, flatulence, and poor appetite. The plant is also used as a relaxant for the gastrointestinal tract and bile ducts and for relieving colic [3], while the observed laxative effect is utilized in the treatment of obesity. It is also used as infusions for external use for alleviating rheumatic and leg pains, as well as for preparing sitz baths for hemorrhoids. Other traditional applications of A. vulgaris include the treatment of nervous system disorders such as insomnia [31], epilepsy, depression, and excessive stress exposure [69]. Furthermore, it is recommended for relieving hypertension and inducing labor or miscarriage [98].

10. Applications in Modern Phytotherapy and Position in Official European Medicine

At present, A. vulgaris herb is not commonly used as a medication. However, due to its aroma and bitter taste, the herb is used for stimulating the secretion of digestive juices in the treatment of appetite loss, achlorhydria, gastritis, and flatulence [3,31]. The roots of A. vulgaris are not rich in bitter components, and therefore, they do not induce gastric secretion.
The essential oil of A. vulgaris is used in insect repellents and fumigants. In addition, it exhibits antibacterial and antifungal properties [3,7,18].
A monograph of the raw material A. vulgaris herba was prepared by the German Commission in 1988. This document listed only the traditional, above-mentioned uses of the herb and emphasized that the effectiveness of preparations based on A. vulgaris had not been confirmed and hence they are not recommended for therapeutic uses [51].
In one of the latest editions of European Pharmacopoeia [52] and the French Pharmacopoeia [53], A. vulgaris is listed as a homeopathic raw material. Its application includes the treatment of irregular menstrual cycles and menopausal symptoms [7], and nervous disorders such as sleepwalking, seizures, epilepsy, and anxiety [99]. The homeopathic medications are prepared using a fresh-root tincture, which is made of 65% ethanol. The tincture should contain a minimum of 0.01% (w/w) derivatives of hydroxycinnamic acid, quantified as the equivalents of chlorogenic acid [100].
In allopathy, mugwort is used mainly in the form of infusions. These are prepared by pouring boiling water over one teaspoon (about 1.2 g) of dried herb and brewing the mixture covered for about 5 min. Another form of mugwort medication used in allopathy is tinctures, which are sold all over Europe [3].
The applications of A. vulgaris documented in scientific works and the pharmacological activity profile of this species recommended in modern phytotherapy are presented in the below sections (Table 5).

11. Biological Activities of Extracts Confirmed by Scientific Research

11.1. Antioxidant Effect

In 2008, scientists from Cairo (Egypt) evaluated for the first time the antioxidant activity of A. vulgaris. They found that the aqueous extract of the herb was able to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals at IC50 = 11.4 μg/mL and nitric oxide (NO) radicals at IC50 = 125 mg/mL in the first part of the experiment. They also estimated total phenols, flavonoids, and flavonols as 19 ± 0.16 mg/g gallic acid equivalents and 7.96 ± 0.76 and 3.4 ± 0.0 mg/g rutoside equivalents, respectively. In the second part of the experiment, they observed a significant increase in the levels of ascorbic acid and glutathione, and an increase in the activity of superoxide dismutase in the blood of rats administered with the extract at a dose of 100 mg/kg body weight (BW). The results of the study showed that A. vulgaris exhibits antioxidant activity and can thus be helpful to treat oxidative stress-related diseases [8].
Some newer investigations documented the antioxidant activity of the whole plant [101], its aerial parts [102] and leaf extracts [59], and its essential oil [120].
Various modern techniques have been applied in the above studies. Oyedemi and Coopoosamy proved the strong antioxidant potential of the extracts of A. vulgaris obtained from South Africa based on lipid peroxidation, protein glycation, xanthine oxidase, and the sTable DPPH radical scavenging assays [101]. Ben Nasr et al. investigated the antioxidant potential of the aqueous extracts of Tunisian A. vulgaris using the following in vitro techniques: ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid assay), DPPH, hydroxyl, superoxide, and NO scavenging assays, ferric reducing power activity assay (for determining total antioxidant capacity), and thiobarbituric acid reactive species assay (for determining the inhibition of lipid peroxidation) [103].

11.2. Hypolipemic Effect

An extract obtained from the root of A. vulgaris was tested by a Hindu team. They analyzed the rats in which hyperlipidemia had been caused by the administration of a high-fat diet for a period of 30 days. In the following month, the rodents received a standard diet. The team found that the A. vulgaris root extract showed a significant lipid profile-normalizing activity. Total cholesterol (TC) was reduced to 180 mg/dL, triglycerides (TG) to 147.2 mg/dL, low-density lipoprotein (LDL) cholesterol to 126.3 mg/dL, and very low-density lipoprotein (VLDL) cholesterol to 28.2 mg/dL, while the level of high-density lipoprotein (HDL) cholesterol and atherogenicity indicator (AI) increased to 68 and 2.63 mg/dL, respectively, compared to the control group (TC = 282.23 mg/dL, TG = 243.2 mg/dL, LDL cholesterol = 209.16 mg/dL, VLDL cholesterol = 47.56 mg/dL, HDL cholesterol = 34.17 mg/dL, and AI = 8.2 mg/dL) [9].
The hypolipidemic and anti-inflammatory effects of the A. vulgaris extract were studied in hypercholesterolemic rats by a Chinese team. They induced hypercholesterolemia in rats by feeding them with a high-fat diet containing 3% cholesterol in olein oil, for 8 weeks. This led to a significant increase in the serum levels of triglycerides, TC, LDL cholesterol, malondialdehyde, NO, and tumor necrosis factor-α and a significant decrease in the serum level of HDL cholesterol, activity of hydroxymethylglutaryl-CoA reductase in the liver, and activity of paraoxonase-1 as compared to the normal control group. Treatment of rats with A. vulgaris extract at a dose of 100 mg/kg per day for 4 weeks normalized the serum lipid profile, significantly increased the paraoxonase-1 activity, and decreased the serum levels of malondialdehyde, NO, and tumor necrosis factor-α as compared to the high-fat diet-treated animals. Moreover, the extract caused a decrease in the activity of hydroxymethylglutaryl-CoA reductase as compared to both high-fat diet-treated animals and control ones [104].

11.3. Hepatoprotective Effect

In 2005, two teams from Pakistani universities in Karachi investigated the hepatoprotective properties of A. vulgaris herb extracts in in vivo studies on mice. They divided the test rodents into five groups and conducted an experiment. The first and second groups were administered with a saline solution; two hours later, the second group received an additional 700 mg/kg d-galactosamine and 1 μg/kg lipopolysaccharide, which caused liver inflammation as confirmed by elevated levels of hepatic function indicators—alanine aminotransferase (ALAT) and aspartate aminotransferase (ASPAT). The third, fourth, and fifth groups were given different, prophylactic, doses (from 150 to 600 mg/kg BW) of a crude hydromethanolic extract prepared from the aerial parts of A. vulgaris, and an hour later, received d-galactosamine and lipopolysaccharide. A significant reduction was noted in ASPAT and ALAT activities in plasma in all the three groups. The results observed in these groups were confirmed by a histopathological examination of the liver, which showed a decrease in cellular edema and apoptotic cell count, and no hyperemia of the hepatic parenchyma, relative to the second group [105].

11.4. Antispasmodic Effect

A group of researchers in Cardiff (UK) tested the extracts prepared from the herb of A. vulgaris for their activity toward biogenic amine receptors in the smooth muscles of the gastrointestinal tract and respiratory tract of guinea pigs. The antagonistic effects of chloroform and methanol extracts were determined using concentration–response curves for contractions of the trachea and the small intestine under the influence of 5-hydroxytryptamine, methacholine, histamine, and phenylethylamine. The team found that both extracts had an antagonistic effect on the H1 receptors and caused the relaxation of smooth muscles. Two main compounds were isolated from the chloroform fraction—yomogin and 1,2,3,4-diepoxy-11(13)-eudesmen-12,8-olide—of which the former showed significant antagonism toward the H1 receptor in the ileum [10].
Researchers in São Paulo (Brazil) studied the antinociceptive activity of A. vulgaris herb extracts and observed their antispasmodic effects. They demonstrated that in mice treated with 500 or 1000 mg/kg hydroethanolic extract, a 48% or 59% inhibition of abdominal contractions was caused by the acetic acid solution, respectively [11].

11.5. Bronchodilatory Effect

Khan and Gilani tested the antispasmodic, bronchodilatory, tracheorelaxant, and antidiarrheal activities of the crude extract of A. vulgaris in the isolated tissue preparations of rabbit jejunum and guinea pig trachea, and also analyzed the in vivo castor oil-induced bronchodilation. They found that the A. vulgaris extract exhibited a combination of anticholinergic and Ca2+ antagonist mechanisms, which seemed pharmacologically promising for the treatment of airways disorders. The authors supposed that alkaloids, coumarins, flavonoids, saponins, sterols, tannins, and terpenes are the compounds responsible for the concentration-dependent (0.03–10 mg/mL) broncholytic effect of the A. vulgaris extract [106].
Natividad et al. proved that the Philippines A. vulgaris medicinal plant exhibited antagonistic activity at selected biogenic amine receptors in the smooth muscles of the airways and gastrointestinal tract. The chloroform and methanol extracts of the plant showed histamine H1 antagonism in the ileum and trachea. The researchers indicated that yomogin (sesquiterpenoid lactone) was the compound responsible for these activities [10].

11.6. Analgesic Effect

The team from São Paulo (Brazil) also investigated the antinociceptive properties of a hydroalcoholic extract prepared from the aerial parts of A. vulgaris using a mice model. Two tests were performed by the researchers: a hot plate test to measure the central analgesic effect and a writhing test to measure the peripheral analgesic effect. In the hot plate test, the control group was given only water, while the test group received 500 or 1000 mg/kg of A. vulgaris extract. Mice given 20 mg/kg BW morphine were adopted as the positive control. The response time (response latency) was determined after placing the rodents on a plate heated to 55 °C. The time taken by the mice to respond was not found to decrease after the administration of the A. vulgaris extract. In the writhing test, the mice were divided into groups and were given water, 20 g/kg BW morphine, and the hydroalcoholic extract of A. vulgaris at doses of 100, 250, 500, and 1000 mg/kg BW. After 30 min, the rodents were administered with a 0.8% acetic acid solution and writhing episodes were determined for the next 10 min. No decrease in the number of episodes was observed with the extract administered at the doses of 100 and 250 mg/kg BW; however, a decrease was observed with the higher doses of the extract (i.e., 500 and 1000 mg/kg BW). The results proved that the tested extracts of the A. vulgaris herb had a moderate peripheral antinociceptive effect but not a central effect [11].

11.7. MAO Inhibitory Effect

Lee et al. (South Korea) isolated flavonoids (jaceosidine, eupafolin, luteolin, quercetin, apigenine) and coumarins (esculetin, esculetin-6-methylether, scopoletin) from 80% aqueous ethanol extracts of whole A. vulgaris plants, and indicated that these compounds are good inhibitors of mouse brain monoamine oxidase (MAO) enzyme. The compounds showed MAO inhibitory effects at the IC50 values of 19.0, 25.0, 18.5, 12.5, 1.0, 31.1, 32.2, and 45.0 µmol, respectively [107].

11.8. Antihypertensive Effect

The aqueous and chloroform extracts of the aerial parts of A. vulgaris were tested by two teams from the Philippines to determine the hemodynamic potential of the plant. They found that the administration of a 10% solution of the aqueous extract into the isolated perfused mesentery of rats effectively reversed the hypertensive effect induced by noradrenaline; however, the baseline blood pressure values and heart rate remained unaltered [12].
The Chinese team performed a systematic review of randomized controlled trials based on a review of articles published from 1980 to 2013 in databases (CENTRAL, Pubmed, CBM, CNKI, VIP, and online clinical trial registry websites). They exhibit that in randomized controlled trials, moxibustion (a traditional Chinese method that uses the heat generated by burning herbal preparations containing A. vulgaris to stimulate acupuncture points) showed lowering of the blood pressure compared to antihypertensive drugs by stimulation of acupoint KI 1. Meta-analysis showed superior effects of moxibustion plus antihypertensive drugs (like: Metoprolol, Nifedipine, Enalapril) on systolic blood pressure (WMD (weighted mean difference): −4.91 [−7.54, −2.28]) but no superior effects on diastolic blood pressure (WMD: −6.38 [−17.17, 4.41]) [108].

11.9. Estrogenic Effect

The flavonoids present in the aerial parts of A. vulgaris were evaluated for estrogenic activity by three scientific institutions from USA and South Korea in cooperation. Saccharomyces cerevisiae fungi containing an expression plasmid with the cDNA of the human estrogen receptor and a reporter plasmid with the α-galactosidase gene was used in the experiment. The estrogenic effect, 5% relative to 17-β-estradiol, was observed in the polar extract (extraction with ethyl acetate), whereas the less polar ethanolic extract showed no activity. After testing the individual flavonoids of the plant, it was observed that the transcription of the reporter gene was significantly induced by eriodictyol and apigenin. The activity was also found to be concentration-dependent [12].
Shaik et al. (India) confirmed the strong anti-implantation and estrogenic activities of the leaf extracts from A. vulgaris on female Wistar rats [109].

11.10. Cytotoxic Effect

A methanolic extract prepared from the aerial parts of A. vulgaris was tested by Turkish teams to analyze its cytotoxic effects on human cancer cell lines (MCF7—estrogen-dependent breast adenocarcinoma cell line, A549—non-small cell lung cancer cell line, HeLa—cervical cancer cell line), and normal cell lines (A7R5—vascular smooth muscle cell line, 293T—human embryonic kidney cell line transformed with SV40 large T antigen). Cytotoxic activity was assessed by real-time electrical impedance measurements. A statistically significant inhibitory effect was observed against MCF7 (IC50 = 190 ng/mL), HeLa (IC50 = 284 ng/mL), A7R5 (IC50 = 382 ng/mL), and 293T (IC50 = 317 ng/mL), whereas a weak influence was found on A549 (IC50 = 778 ng/mL) cells [14]. Saleh et al. from Saudi Arabia evaluated the toxicity of the essential oil extracted from the buds and leaves of the A. vulgaris plant, and characterized its growth inhibitory effects on cancer cells. The results demonstrated that the essential oil-induced apoptosis in HL-60 leukemic cell line was mediated by caspase-dependent pathways, involving caspase-3, -9, and -8, which were initiated by Bcl-2/Bax/Bid-dependent loss of mitochondrial membrane potential, leading to the release of cytochrome c to the cytoplasm to activate the caspase cascade. The results showed that the studied essential oils were more efficient in inducing apoptosis in different cancer cell lines than noncancerous cells. Based on these observations, the authors suggested that A. vulgaris might be a promising source of new anticancer agents [111].
The latest study from 2020 was performed by a Serbian team on methanolic extracts of the A. vulgaris plant. The researchers proved the genotoxic and cytotoxic activities of the extracts, both separately and in co-treatment with a known mutagen (mitomycin C). They used the cytokinesis-block micronucleus assay for measuring the micronucleus frequency in human peripheral blood lymphocytes and MTT assay as the proliferation test in SW-480 human colon cancer cells and human periodontal ligament stem cells used as control. The results of the cytokinesis-block micronucleus assay showed that both extracts significantly increased the micronucleus frequency in the peripheral blood lymphocytes treated with the A. vulgaris extract at all the tested concentrations (10, 50, 100, and 250 μg/mL), except the lowest one (10 μg/mL). The extracts induced cytotoxic activity only in co-treatment with mitomycin C after long-term exposure and did not significantly affect the viability of the human periodontal ligament stem cells. The researchers indicated that these activities were induced by the flavonoids and other phenolic compounds present in the plant [110].

11.11. Antifungal and Antibacterial Activities

Researchers from Romania investigated the antifungal activity of a commercial preparation of the essential oil extracted from the A. vulgaris herb against Candida albicans. The disk-diffusion method was used in the in vitro experiment. The observed inhibition zone measured 12.5 mm. The zone of the positive control, carried out with nystatin, measured 15.0 mm, while that of the negative control, carried out with an empty paper disc, measured 6.0 mm. The results thus confirmed the antifungal activity of the essential oil of the herb against C. albicans [15].
In 2006, Blagojević et al. from Serbia and Montenegro also studied the effects of A. vulgaris essential oil on various microorganisms. The oil was isolated from the aerial and underground parts of the plant by steam distillation. After 10- and 30-fold dilutions, the zone of inhibition of pathogen growth on paper filters was examined. The oil extracted from the aerial parts exhibited inhibitory activity against various bacteria (Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus) and fungi (C. albicans and Aspergillus niger), which was attributed to its high levels of 1,8-cineole and β-thujone. On the other hand, the oil extracted from the underground parts of the plant exhibited only a low activity against the listed pathogens, due to the low level of 1,8-cineole and the lack of β-thujone in the roots [16].
In another experiment, an aqueous leaf extract of A. vulgaris was found to exhibit antibacterial activity against the C and D serotypes of Streptococcus mutans [3].
Some recent studies also documented the antimicrobial activity of the whole plant and leaf extracts [102,113,121] as well as the essential oil of A. vulgaris [67,112].

11.12. Anti-Inflammatory Activity

El-Tantawy from Pakistan tested the anti-inflammatory and hypolipidemic effects of A. vulgaris extract in hypercholesterolemic rats. Hypercholesterolemia was induced in the rats by feeding them with a high-fat diet containing 3% cholesterol in olein oil, for 8 weeks. Treatment of these rats with A. vulgaris extract at a dose of 100 mg/kg per day for 4 weeks normalized the serum lipid profile, significantly increased the paraoxonase-1 activity, and decreased the serum levels of malondialdehyde, NO, and tumor necrosis factor-α as compared to the animals that received only high-fat diet. Moreover, the extract caused a significant decrease in the activity of hydroxymethylglutaryl-CoA reductase as compared to both high-fat diet-treated animals and control ones [104].
Afsar et al. from India studied the anti-inflammatory activity of a methanolic extract of A. vulgaris leaves using the cotton pellet granuloma method. The A. vulgaris extract was administered to rats (150–250 g BW) at doses of 200 and 400 mg/kg BW, respectively, after surgical insertion of cotton pellets into their groin region. The study was conducted following the guidelines of the “cotton pellet granuloma method.” The extract showed anti-inflammatory activity at both 200 and 400 mg/kg, whereas the results were more significant at 400 mg/kg compared with the control [115].
The latest study performed on the extracts of Tunisian A. vulgaris plants proved their moderate anti-inflammatory activity based on lipoxygenase inhibition assay [103].

11.13. Antiallergenic Effect

Olsen et al. from Denmark studied the specificity and efficacy of immunotherapy with A. vulgaris extracts on 25 patients having seasonal rhinoconjunctivitis for more than two years and only two clinically important allergies—either to A. vulgaris and Betula verrucosa or to A. vulgaris and Phleum pratense. Twenty patients completed two years of specific immunotherapy. Nine patients were treated with the extracts of A. vulgaris and 11 with the extracts of either B. verrucosa or P. pratense. Treatment with A. vulgaris was followed by a significant decrease in skin and eye sensitivity to A. vulgaris but not to B. verrucosa or P. pratense. No significant decrease was observed in medicine consumption or symptom scores. The patients treated with B. verrucosa or P. pratense experienced a significant decrease in skin and eye sensitivity to these organisms, but not to A. vulgaris, and showed a significant decrease in medicine consumption and symptom scores in the B. verrucosa or P. pratense season but not in the A. vulgaris season. The treatment was both effective and specific, with one unexplained exception that both patient groups (A. vulgaris and B. verrucosa or P. pratense) experienced decreased skin sensitivity to A. vulgaris [116].

11.14. Antimalarial Activity

Kodippili et al. from Sri Lanka investigated the antimalarial (both antiparasitic and antidisease) activity of the A. vulgaris extract. They assessed the antiparasitic activity of the extract at three doses (250, 500, and 1000 mg/kg) in vivo in the Plasmodium yoelii rodent malaria model, using distilled water as the negative control and Coartem as the positive control. In the 4-day suppressive assay, the oral administration of the extract at 500 and 1000 mg/kg significantly inhibited parasitemia by 65.16% and 51.46%, respectively. The antinociceptive activity of the extract was analyzed using the hot plate test, which indicated a central, supraspinally mediated response in relieving pain. The antidisease activity of the extract was further corroborated by the increased survival of the infected mice treated with the 500 mg/kg dose. The A. vulgaris extract was well tolerated by the mice over a period of 14 days (assay of subchronic toxicity), with no overt signs of toxicity or stress. Hepatotoxicity (evaluated in terms of the serum levels of glutamic–oxaloacetic transaminase and serum glutamic–pyruvic transaminase), renotoxicity (in terms of serum urea and creatinine), and hematotoxicity (in terms of total RBC, WBC, and differential leukocyte counts) were also ruled out. Based on these findings, the authors concluded that A. vulgaris leaf extract is orally active and nontoxic, and that A. vulgaris is a weed with the potential to act as a cheap source of the antimalarial plant [117].
Another team from the same center from Sri Lanka studied the ethanolic leaf extract of A. vulgaris for antiparasitic activity in Plasmodium berghei ANKA murine malaria model that elicits similar pathogenesis as falciparum malaria. The extract at doses of 500, 750, and 1000 mg/kg significantly inhibited parasitemia by 79.3%, 79.6%, and 87.3% respectively, in the 4-day suppressive assay. Chronic administration of the extract at high dose ruled out the overt signs of toxicity and stress as well as hepatotoxicity, renotoxicity, and hematotoxicity. Therefore, the authors claimed that the oral administration of a crude extract of A. vulgaris is nontoxic and induces antimalarial (antiparasitic) effects [118].

11.15. Anthelmintic Activity

Caner et al. from Turkey proved that the extracts of A. vulgaris and A. absinthium induce anthelmintic effects against trichinellosis (Trichinella spiralis) in rats. The results of trichinoscopy and artificial digestion showed that during the enteral (adult) phase of the illness, 300 mg/kg of methanol extracts prepared from the aerial parts of A. vulgaris and A. absinthium reduced the larval rate by 75.6% and 63.5% in the tongue, 53.4% and 37.7% in the diaphragm, 67.8% and 46.2% in the quadriceps, and 66.7% and 60.5% in the biceps–triceps muscles of rats, respectively. Furthermore, during the parenteral (encapsulated larvae) phase, 600 mg/kg dose of both the plant extracts decreased the larval rate by 66.4% and 59.9% in the tongue, 57.4% and 50.0% in the diaphragm, 47.6% and 43.7% in the quadriceps, and 60.2% and 46.4% in the biceps–triceps muscles of rats, respectively. In addition, antibody analysis showed that A. vulgaris extract can significantly reduce the antibody response [119].

11.16. Insecticidal Activity

The insecticidal activity of A. vulgaris was studied by a Hindu team from Tiruchirappalli in India. They exposed the larvae of Aedes aegypti (Egyptian mosquito, dengue virus vector) to various concentrations of the essential oil extracted from the A. vulgaris herb. Even at a low concentration of 10 ppm, insecticidal activity was observed after 24 h, while the best results were seen at a concentration of 500 ppm, where after 8 h of exposure to the oil solution, the larvae mortality rate was 100%. Thus, the study showed that the essential oil of A. vulgaris might serve as a potential insecticide [17].
The activity of essential oil obtained from the leaves and nanoparticles prepared with leaf extracts was evaluated by Lavor et al. from Brazil and Balasubramani et al. from India [122,123].
Furthermore, other teams have documented the effect of different raw materials against another insect species—Culex quinquefasciatus [124] and some stored product insects [125] (Table 6).

12. Applications in Cosmetology

Based on its profile of activity, the European database of cosmetic raw materials, CosIng (Cosmetic Ingredients database), recommends the use of A. vulgaris in eight forms, including skincare agents, humectants, skin protectants, and fragrances. Original forms used in cosmetics are filtrates obtained as a result of fermentation by bacteria (Bacillus sp., Lactobacillus sp.) or fungi (Saccharomyces sp.) (Table 7).
During fermentation, Bacillus sp. produce valuable physiologically active substances such as peptides, viscous compounds (with polysaccharide structure), antioxidants, and fibrin.
A combination of A. vulgaris and Bacillus sp. has been shown to enhance the effects of fermentation and increase the antiaging and antiwrinkle effects by inhibiting the production of matrix metalloproteinase-1 and -9 enzymes (decomposed of collagen) and increasing cell regeneration and collagen synthesis [127].
In cosmetics production, the extracts of herb and essential oil are used, which are the raw materials that do not contain pollen causing allergic reaction.
Cosmetic products containing A. vulgaris extracts are available on the European, Asian, and American markets. The species is of particular interest to South Korean companies such as Hanyul, Missha, Isntree, I’m From, Round lab, VelyVely, Dr. Jart+, and Tonymoly. They produce cosmetics (mainly in the form of creams) using the extracts of A. vulgaris herb, which are intended for skincare. In Russia, PervoeReshenie, Etude House, Natura Siberica, and Fitocosmetic use A. vulgaris in the production of, for example, toothpastes, balms, and creams. The plant extracts are also found in the products sold by American cosmetic companies, including Soleil Toujours, AoSkincare, and TruBaby.

13. Applications in the Food Industry

The essential oil extracted from the aerial parts of A. vulgaris is useful in the food industry, as it exhibits antiseptic, antioxidant, and antimicrobial activities. In addition, the oil has larvicidal, nematicidal, and pesticidal effects [46].
The species A. vulgaris is highly valued as a spice because of the aroma and bitter taste of the herb and the sweet and spicy taste of the roots. The leaves and buds collected just before flowering are used as a bitter additive for seasoning rice dishes and tea in Asia [14,18]. The species is also used as an additive to meat, poultry, fish, and salads, in the production of vodkas and herbal wines [18], and in the preparation of sweet and savory cakes (especially in Japan). Before the introduction of hops, A. vulgaris had been used to flavor beer [18].
Despite its earlier popularity, A. vulgaris is relatively rarely used as a spice in Poland. Currently, there are attempts being made to make it more popular as a seasoning agent for, inter alia, mutton, liver, cabbage, spinach, and mushroom dishes, as well as soups.
In addition, A. vulgaris tincture is widely used in animal feed as a sensory (aromatic) additive [128].
The EFSA reports that as an insecticide A. vulgaris is intended for use in arable fields, for protecting plants in orchards and vineyards, and for growing vegetables. The plant targets insects such as Ae. aegypti (Egyptian mosquito), Musca domestica (housefly), and Tribolium castaneum (red flour beetle). There is increasing interest in using the plant for this purpose because it is less harmful to both humans and the environment compared to other preparations [18,46,129].

14. Safety of Use

The use of A. vulgaris herb extracts in therapeutic doses is not likely to cause side effects; however, the plant can cause allergies, as confirmed by the U.S. Food and Drug Administration (FDA). Its pollen contains allergenic glycoproteins that cause the type I (immediate) allergic reaction, related to IgE antibodies. In addition, anaphylactic shock has been observed in patients after swallowing the pollen [3,130]. The species is also considered to be the main cause of hay fever and allergic asthma in northern Europe, North America, and part of Asia [18,131].
If a person is allergic to any ingredient of A. vulgaris or any plant of the family Asteraceae, he/she should avoid contact with them. Cross-reactions of the plant with pollen from other plants as well as with food substances have also been observed—with birch, cabbage, grasses, hazelnuts, honey, pollen of the European olive and sweet pepper, and also with royal jelly, sunflower, kiwi, peach, mango, apple, celery, and carrot [18,132].
In addition to anaphylactic shock, A. vulgaris pollen, as an allergen, can cause breathing difficulties, bronchospasm, airway hypersensitivity, asthma attack, seasonal rhinitis, and conjunctivitis. Allergic skin reactions, such as dermatitis and urticaria, may also occur [92,131,133,134].
When consumed in large doses, A. vulgaris may cause miscarriage [51,92], nausea, vomiting, and nervous system damage. Furthermore, cases of hypertension have been reported [18].
The EFSA lists that the ingredients in the essential oil of A. vulgaris herb, such as α-thujone, β-thujone, camphor, and 1,8-cineole, have potentially adverse effects on human health, when taken with food or dietary supplements [135]; however, it emphasizes that most research has concerned only a more concentrated oil, and not a less concentrated extract [18].
It should be remembered that A. vulgaris should be used with caution in patients with diabetes, as it can increase blood glucose levels. The species is also not recommended for the prevention of malaria and in vitro studies have not shown protozoicidal activity. In addition, it was reported that patients who used A. vulgaris as a prophylactic agent when traveling to eastern Africa were not prevented from developing malaria [18].
The EFSA has also published a document describing the safety of using a hydroethanolic tincture of A. vulgaris having a dry matter content of approximately 1.7% as an animal feed additive. The ruling emphasizes that although the isolated phenolic compounds are not toxic, the safety profile of this tincture cannot be determined because as much as 74% of the dry matter fraction remains undetermined. It is also not known whether the tincture can cause irritation to the skin or eyes. The document is summarized with a statement declaring that no further research is needed because A. vulgaris and its extracts are commonly used as aromatic substances in food, where they perform similar functions as in feed [128].
Scientists from four Japanese centers—Science University of Tokyo (Tokyo), The Institute of Physical and Chemical Research (RIKEN) (Wakō), Nagoya University School of Medicine (Nagoya), and Aichi Cancer Center Research Institute (Nagoya)—have isolated prunasin from A. vulgaris (Figure 4). They used 300 mg of acetone extract and obtained 25 mg of prunasin [63]. Prunasin is a cyanogenic glycoside that releases toxic hydrogen cyanide (HCN) during enzymatic hydrolysis by β-3-glucosidases in the macerated plant tissue or under the influence of intestinal microflora. In the human body, HCN combines with cytochrome oxidases and prevents oxygen access to tissues, thereby leading to body hypoxia [136]. However, further research is needed on the prunasin content in A. vulgaris and its effect on the human body.

15. Biotechnological Research on Micropropagation

Excessive exploitation and exposure to anthropogenic activities can cause a change in the chemical composition of A. vulgaris. In addition, the species rarely reproduces from seeds, which require symbiosis with soil microflora for germination. An alternative way of reproduction for the plant is micropropagation—a technique used for rapid multiplication in vitro. It provides conditions under which the progeny plant is genetically stable and its metabolites are identical in chemical composition to that of the parent plant [69].
At the University of Mysore in Karnataka (India), Sudershana et al. [44] investigated the effect of different phytohormone concentrations on the micropropagation of A. vulgaris in 2010. They used young leaf explants and the Murashige-Skoog (MS) medium enriched with 1 mg/l 6-benzylaminopurine (BAP) and 3 mg/L 1-naphthylacetic acid, which proved optimal for inducing callus growth. The callus was then transferred to a medium containing 1 mg/L BAP and 3.0 mg/L gibberellic acid (GA3), which was found to be optimal for shoot development. The addition of indolylacetic acid (IAA) at 0.5–1 mg/L induced root development [44].
In 2013, the same group of scientists developed a protocol for regenerating A. vulgaris from encapsulated somatic embryos (so-called somatic seeds). The plant development was observed to be best when encapsulation was done with a 2% alginate solution cured for 30 min in a 75 mM calcium chloride solution. It was proved that the encapsulated embryos of A. vulgaris could be stored at 4 °C, 20 °C, and 22 °C for 4 months. These embryos showed the highest (90%) efficiency of conversion into microplants at 22 °C on MS medium containing 1.5 mg/L GA3, 0.5 mg/L IAA, and 40 mg/L ascorbic acid [137].
An effective protocol for the micropropagation of A. vulgaris was developed by scientists from Bharathidasan University in Tamil Nadu (India) in cooperation with Universita di Pisa in Pisa, (Italy). The method involved in vitro liquid culturing on MS medium with the addition of BAP at concentrations from 0.44 to 8.88 μM. In 100 mL flasks, the frequency of shoot formation from explants was determined to be 99.9% when the BAP concentration was 4.44 μM. The largest number of shoots (85.5) was obtained after 30 days of culture in 500 mL flasks, with 4.44 μM BAP. An increase in shoot proliferation with an increase in flask capacity and a decrease in proliferation were observed with an increase in the BAP concentration above 4.44 μM. In the next stage of work, individual shoots were isolated and rooted on MS medium with 8.56 μM IAA. The seedlings obtained were acclimatized under standard laboratory conditions. The plants thus grown were then transferred to the greenhouse [64].

16. Conclusions

Artemisia vulgaris, a species which played a significant role in the history of European medicine, was referred to in the Middle Ages as the “mother of herbs,” and used inter alia in treating gynecological and urological ailments and gastrointestinal tract diseases, is not a pharmacopoeial species today in the global allopathic medicine. However, it occupies an important position in traditional medicine, both in Europe and Asian countries, mainly China and India.
Currently, A. vulgaris is the subject of numerous phytochemical and pharmacological studies. Phytochemical studies have proved the rich composition of the aerial parts of this plant, which consists of sesquiterpenoid lactones, flavonoids, and coumarins, and an essential oil made of qualitatively variable components. In turn, pharmacological studies have provided evidence of very valuable, previously unknown biological activities of the raw materials of A. vulgaris, including antioxidant, hypolipemic, hepatoprotective, antispasmodic, analgesic, antihypertensive, estrogenic, cytotoxic, antibacterial, and antifungal effects. Due to the high variability in its chemical composition, this species is also subject to biotechnological research, where attempts are being made to genotypically multiply high-production plants using micropropagation methods. A major part of this research is conducted by non-European research centers. This is because the species is widely distributed on as many as four continents—Europe, Asia, North America, and South America—and there is a slightly greater interest in this species outside of Europe.
In East Asian countries, mainly China and Japan, as well as in Europe, this species is well known as a spice. In Europe, the current interest in the species as a spice is definitely not as strong as in Asia; however, it has been increasingly used in the cosmetics industry.
The biological activities of the A. vulgaris raw material proven so far have raised hopes for a renaissance of the interest of contemporary medical world in the medieval “mother of herbs”.

Author Contributions

Data collection: H.E., J.P., P.K., A.R., H.Ś., A.S.; design of the study: H.E.; analysis and interpretation of the data: H.E., J.P., P.K., A.R., H.Ś., A.S.; drafting the manuscript: H.E., J.P., P.K., A.R., H.Ś., A.S.; critical revision of the manuscript: H.E., A.S. All authors read and approved the manuscript in its final form. All authors have read and agreed to the published version of the manuscript.


This research was funded by grand numbers: N42/DBS/000010 and N42/DBS/000136 supported by the Polish Ministry of Science and Higher Education.

Conflicts of Interest

The authors declare no conflict of interest.


  1. Haq, F.U.; Roman, M.; Ahmad, K.; Rahman, S.U.; Shah, S.M.A.; Suleman, N.; Ullah, S.; Ahmad, I.; Ullah, W. Artemisia annua: Trials are needed for COVID-19. Phyther. Res. 2020. [Google Scholar] [CrossRef] [PubMed]
  2. Efferth, T.; Zacchino, S.; Georgiev, M.I.; Liu, L.; Wagner, H.; Panossian, A. Nobel Prize for artemisinin brings phytotherapy into the spotlight. Phytomedicine Int. J. Phyther. Phytopharm. 2015, 22, 1–4. [Google Scholar] [CrossRef] [PubMed]
  3. Wichtl, M. Herbal Drugs and Phytopharmaceuticals: A Handbook for Practice on a Scientific Basis, 3rd ed.; Medpharm: Marburg, Germany, 2004. [Google Scholar]
  4. The Plant List. Available online: (accessed on 10 March 2020).
  5. Holm, L.; Doll, J.; Holm, E.; Pnacho, J.; Herberger, J. World Weeds: Natural Histories and Distribution; John Wiley and Sons: New York, NY, USA, 1997. [Google Scholar]
  6. Chevallier, A. The Encyclopedia of Medicinal Plants: A Practical Reference Guide to more than 500 Key Medicinal Plants and Their Uses; DK Publishing: New York, NY, USA, 1996. [Google Scholar]
  7. Barney, J.N.; DiTommaso, A. The biology of Canadian weeds. 118. Artemisia vulgaris L. Can. J. Plant. Sci. 2003, 83, 205–215. [Google Scholar] [CrossRef]
  8. Temraz, A.; El-Tantawy, W.H. Characterization of antioxidant activity of extract from Artemisia vulgaris. Pak. J. Pharm. Sci. 2008, 21, 321–326. [Google Scholar] [PubMed]
  9. Khan, K.A. A preclinical antihyperlipidemic evaluation of Artemisia vulgaris root in diet induced hyperlipidemic animal model. Int. J. Pharmacol. Res. 2015, 5, 110–114. [Google Scholar]
  10. Natividad, G.M.; Broadley, K.J.; Kariuki, B.; Kidd, E.J.; Ford, W.R.; Simons, C. Actions of Artemisia vulgaris extracts and isolated sesquiterpene lactones against receptors mediating contraction of guinea pig ileum and trachea. J. Ethnopharmacol. 2011, 137, 808–816. [Google Scholar] [CrossRef]
  11. Pires, J.M.; Mendes, F.R.; Negri, G.; Duarte-almeida, J.M.; Carlini, E.A. Antinociceptive Peripheral Effect of Achillea millefolium L. and Artemisia vulgaris L.: Both Plants known popularly by Brand Names of Analgesic Drugs. Phyther. Res. 2009, 219, 212–219. [Google Scholar] [CrossRef]
  12. Tigno, X.T.; de Guzman, F.; Flora, A.M.; Theresa, V. Phytochemical analysis and hemodynamic actions of Artemisia vulgaris L. Clin. Hemorheol. Microcirc. 2000, 23, 167–175. [Google Scholar]
  13. Lee, S.J.; Chung, H.Y.; Maier, C.G.A.; Wood, A.R.; Dixon, R.A.; Mabry, T.J. Estrogenic Flavonoids from Artemisia vulgaris L. J. Agric. Food Chem. 1998, 46, 3325–3329. [Google Scholar] [CrossRef]
  14. Erel, B.; Aydin, F.; Ballar, P. In vitro cytotoxic properties of six Artemisia L. species. Turkish J. Pharm. Sci. 2011, 8, 247–251. [Google Scholar]
  15. Obistioiu, D.; Cristina, R.T.; Schmerold, I.; Chizzola, R.; Stolze, K.; Nichita, I.; Chiurciu, V. Chemical characterization by GC-MS and in vitro activity against Candida albicans of volatile fractions prepared from Artemisia dracunculus, Artemisia abrotanum, Artemisia absinthium and Artemisia vulgaris. Chem. Cent. J. 2014, 8, 6. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Blagojević, P.; Radulović, N.; Palić, R.; Stojanović, G. Chemical composition of the essential oils of Serbian wild-growing Artemisia absinthium and Artemisia vulgaris. J. Agric. Food Chem. 2006, 54, 4780–4789. [Google Scholar] [CrossRef] [PubMed]
  17. Govindaraj, S.; Ranjitha Kumari, B.D. Composition and Larvicidal Activity of Artemisia vulgaris L. Stem Essential Oil against Aedes Aegypti. Jordan J. Biol. Sci. 2013, 6, 11–16. [Google Scholar] [CrossRef] [Green Version]
  18. European Food Safety Authority. Artemisia Vulgaris Basic Substance Application; EFSA Supporting Publications: Parma, Italy, 2013. [Google Scholar]
  19. European Commission. CosIng-Cosmetic Database. Available online: (accessed on 8 March 2020).
  20. Anwar, S.; Asif, N.; Naqvi, S.A.H.; Malik, S. Evaluation of multiple risk factors involved in the development of diabetic retinopathy. Pakistan J. Med. Sci. 2019, 35, 156–160. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  21. Abiri, R.; Silva, A.L.M.; de Mesquita, L.S.S.; de Mesquita, J.W.C.; Atabaki, N.; de Almeida, E.B.; Shaharuddin, N.A.; Malik, S. Towards a better understanding of Artemisia vulgaris: Botany, phytochemistry, pharmacological and biotechnological potential. Food Res. Int. 2018, 109, 403–415. [Google Scholar] [CrossRef]
  22. Abad, M.J.; Bedoya, L.M.; Apaza, L.; Bermejo, P. The Artemisia L. genus: A review of bioactive essential oils. Molecules 2012, 17, 2542–2566. [Google Scholar] [CrossRef] [Green Version]
  23. Tan, R.X.; Zheng, W.F.; Tang, H.Q. Biologically Active Substances from the Genus Artemisia. Planta Med. 1998, 64, 295–302. [Google Scholar] [CrossRef] [Green Version]
  24. Bora, K.S.; Sharma, A. The genus Artemisia: A comprehensive review. Pharm. Biol. 2011, 49, 101–109. [Google Scholar] [CrossRef] [Green Version]
  25. Hegnauer, R. Chemotaxonomie der Pflanzen; Springer: Basel, Switzerland, 1962. [Google Scholar]
  26. Tutin, T.G.; Heywood, V.H.; Burges, N.A.; Valentine, D.H. Flora Europaea: Volume 4. Plantaginaceae to Composite (and Rubiaceae); Cambridge University Press: Cambridge, UK, 1976; Volume 4. [Google Scholar]
  27. Szafer, W.; Kulczyński, S.; Pawłowski, B. Rośliny Polskie. Vol. I-III.; Państwowe Wydawnictwo Naukowe: Warszawa, Poland, 1986. [Google Scholar]
  28. Zarzycki, K.; Kaźmierczakowa, R.; Mirek, Z. Polska Czerwona Księga Roślin. Paprotniki i rośliny kwiatowe; Wyd. III.; Instytut Ochrony Przyrody PAN: Kraków, Poland, 2014. [Google Scholar]
  29. Missouri Botanical Garden. Available online: (accessed on 7 March 2020).
  30. Weston, L.A.; Barney, J.N.; DiTommaso, A. A review of the biology and ecology of three invasive perennials in New York State: Japanese knotweed (Polygonum cuspidatum), mugwort (Artemisia vulgaris) and pale swallow-wort (Vincetoxicum rossicum). Plant. Soil 2005, 277, 53–69. [Google Scholar] [CrossRef]
  31. van Wyk, B.-E.; Wink, M. Medicinal Plants of the World; Timber Press: Portland, OR, USA, 2004. [Google Scholar]
  32. Vallès, J.; McArthur, D.E. Artemisia Systematics and Phylogeny: Cytogenetic and Molecular Insights. In Shrubland Ecosystem Genetics and Biodiversity: Proceedings; McArthur, E.D., Fairbanks, D.J., Eds.; Department of Agriculture, Forest Service, Rocky Mountain Research Station: Ogden, UT, USA, 2001; pp. 13–15. [Google Scholar]
  33. Oliva, M.; Vallès, J. Karyological studies in some taxa of the genus Artemisia (Asteraceae). Can. J. Bot. 1994, 72, 1126–1135. [Google Scholar]
  34. Tabur, S.; Civelek, Ş.; Öney, S.; Ergün, Ş.B.Y. Chromosome counts and karyomorphology of some species of Artemisia (Asteraceae) from Turkey. Turk. J. Bot. 2012, 36, 235–246. [Google Scholar]
  35. Vallès, J.; Garcia, S.; Hidalgo, O.; Martín, J.; Pellicer, J.; Sanz, M.; Garnatje, T. Biology, genome evolution, biotechnological issues and research including applied perspectives in Artemisia (Asteraceae). In Advances in Botanical Research; Academic Press Inc.: Cambridge, MA, USA, 2011; Volume 60, pp. 349–419. [Google Scholar]
  36. Gupta, R.C.; Goyal, H.; Singh, V. Cytology of the genus Artemisia (Anthemidae, Asteraceae) in the Western Himalayas. Biologia 2014, 69, 1134–1141. [Google Scholar] [CrossRef]
  37. Pellicer, J.; Garcia, S.; Garnatje, T.; Hidalgo, O.; Korobkov, A.A.; Dariimaa, S.; Vallès, J. Chromosome counts in Asian Artemisia L. (Asteraceae) species: From diploids to the first report of the highest polyploid in the genus. Bot. J. Linn. Soc. 2007, 153, 301–310. [Google Scholar] [CrossRef]
  38. Korobkov, A.A.; Kotseruba, V.V.; Probatova, N.S. Chromosome numbers of some species of Artemisia L. from Altai region, South Siberia. Bot. Pacifica 2014, 3, 61–66. [Google Scholar] [CrossRef] [Green Version]
  39. Koul, M.L.H. Cytogenetics of Polyploids I. Cytology of polyploid Artemisia vulgaris. Cytologia 1964, 29, 407–414. [Google Scholar] [CrossRef] [Green Version]
  40. Xirau, J.V.; Siljak-Yakovlev, S. Cytogenetic studies in the genus Artemisia L. (Asteraceae): Fluorochrome-banded karyotypes of five taxa, including the Iberian endemic species Artemisia barrelieri Besser. Can. J. Bot. 1997, 75, 595–606. [Google Scholar] [CrossRef]
  41. Kim, G.B.; Lim, C.E.; Kim, J.S.; Kim, K.; Lee, J.H.; Yu, H.J.; Mun, J.H. Comparative chloroplast genome analysis of Artemisia (Asteraceae) in East Asia: Insights into evolutionary divergence and phylogenomic implications. BMC Genomics 2020, 21, 415. [Google Scholar] [CrossRef]
  42. Barney, J.N.; Hay, A.G.; Weston, L.A. Isolation and characterization of allelopathic volatiles from mugwort (Artemisia vulgaris). J. Chem. Ecol. 2005, 31, 247–265. [Google Scholar] [CrossRef]
  43. The Herb Society of America. Artemisia—An Essential Facts. 2014. Available online: (accessed on 7 March 2020).
  44. Borzabad, R.K.; Sudarshana, M.S.; Niranjan, M.H. In vitro Plant Regeneration from Leaf Explants of Artemisia vulgaris L.–A Medicinal Herb. Mod. Appl. Sci. 2010, 4, 130–134. [Google Scholar] [CrossRef]
  45. Uva, R.H.; Neal, J.C.; DiTomaso, J.M. Weeds of the Northeast; Cornell University Press: Ithaca, NY, USA, 1997. [Google Scholar]
  46. Anwar, F.; Ahmad, N.; Alkharfy, K.M.; Gilani, A.H. Mugwort (Artemisia vulgaris) oils. In Essential Oils in Food Preservation, Flavor and Safety; Academic Press: London, UK, 2016; pp. 573–579. ISBN 9780124166448. [Google Scholar]
  47. Garnock-Jones, P.J. Floret specialization, seed production and gender in Artemisia vulgaris L. (Asteraceae, Anthemideae). Bot. J. Linn. Soc. 1986, 92, 285–302. [Google Scholar] [CrossRef]
  48. Gleason, H.A.; Cronquist, A. Manual of Vascular Plants of Northeastern United States and Adjacent Canada, 2nd ed.; The New York Botanical Garden: New York, NY, USA, 1991. [Google Scholar]
  49. Pawłowski, F.; Kapeluszny, T.; Kolasa, A.; Lecyk, Z. Fertility of some species of ruderal weeds. Ann. Univ. Mariae Curie-Slodowska 1968, 22, 221–223. [Google Scholar]
  50. Hale, M. Allelopathic potential of Artemisia vulgaris rhizomes. Plant. Physiol. 1982, 69, S126. [Google Scholar]
  51. Bundesinstitut für Arzneimittel und Medizinprodukt. German Commission E Monograph; Blaumenthal, M.T., Hall, R., Rister, B., Eds.; American Botanical Council: Austin, TX, USA, 1988. [Google Scholar]
  52. European Directorate for the Quality of Medicine. European Pharmacopoeia 10.0; Council of Europe: Strasbourg, France, 2020. [Google Scholar]
  53. Française Pharmacopée. Pharmacopée Française, 11th ed.; Noculak, A., Ed.; Georg Olms Verlag: Hildesheim, France, 2020; Volume 37. [Google Scholar]
  54. Marco, J.A.; Sanz, T.J.; Del Hierro, P. Two eudesmane acids from Artemisia vulgaris. Pytochemistry 1991, 30, 2403–2404. [Google Scholar] [CrossRef]
  55. Geissmax, T.A.; Ellestad, A. Vulgarin, a Sesquiterpene Lactone from Artemisia vulgaris L. J. Org. Chem. 1961, 27, 1855–1859. [Google Scholar] [CrossRef]
  56. Numonov, S.; Sharopov, F.; Salimov, A.; Sukhrobov, P.; Atolikshoeva, S.; Safarzoda, R.; Habasi, M.; Aisa, H. Assessment of Artemisinin Contents in Selected Artemisia Species from Tajikistan (Central Asia). Medicines 2019, 6, 23. [Google Scholar] [CrossRef] [Green Version]
  57. Nganthoi, M.; Sanatombi, K. Artemisinin content and DNA profiling of Artemisia species of Manipur. South Afr. J. Bot. 2019, 125, 9–15. [Google Scholar] [CrossRef]
  58. Lee, K.H.; Jung, M.Y.; Kim, S.Y. Effects of Ascorbic Acid on the Light-Induced Riboflavin Degradation and Color Changes in Milks. J. Agric. Food Chem. 1998, 46, 407–410. [Google Scholar] [CrossRef]
  59. Melguizo-Melguizo, D.; Diaz-de-Cerio, E.; Quirantes-Piné, R.; Švarc-Gajić, J.; Segura-Carretero, A. The potential of Artemisia vulgaris leaves as a source of antioxidant phenolic compounds. J. Funct. Foods 2020, 5, 192–200. [Google Scholar] [CrossRef]
  60. Wallnofer, B.; Hofner, O.; Greger, H. Polyacetylenes from the ArtemisiaVulgares’ group. Phytochemistry 1989, 28, 2687–2691. [Google Scholar] [CrossRef]
  61. Carnat, A.; Heitz, A.; Fraisse, D.; Carnat, A.P.; Lamaison, J.L. Major dicaffeoylquinic acids from Artemisia vulgaris. Fitoterapia 2000, 71, 587–589. [Google Scholar] [CrossRef]
  62. Judžentien, A.; Buzelyte, J. Chemical composition of essential oils of Artemisia vulgaris L. (mugwort ) from North Lithuania. Chemija 2006, 17, 12–15. [Google Scholar]
  63. Mizushina, Y.; Takahashi, N.; Ogawa, A.; Tsurugaya, K.; Koshino, H.; Takemura, M.; Yoshida, S.; Matsukage, A.; Sugawara, F.; Sakaguchi, K. The cyanogenic glucoside, prunasin (D-mandelonitrile-β-D-glucoside), is a novel inhibitor of DNA polymerase β. J. Biochem. 1999, 126, 430–436. [Google Scholar] [CrossRef] [PubMed]
  64. Govindaraj, S.; Kumari, B.D.R.; Cioni, P.L.; Flamini, G. Mass propagation and essential oil analysis of Artemisia vulgaris. J. Biosci. Bioeng. 2008, 105, 176–183. [Google Scholar] [CrossRef] [PubMed]
  65. Madhav, K.; Kunal, M.; Zafar, H.; Ujjwal, B.; Gaurav, N. Antioxidant analysis of essential oils and methanolic extracts of Artemisia vulgaris. Int. J. Agric. Sci. 2018, 10, 5710–5713. [Google Scholar]
  66. El-Sahhar, K.F.; Nassar, R.M.; Farag, H.M. Morphological and anatomical studies of Artemisia vulgaris L. (Asteraceae) II. Anatomical characteristics and volatile oil. Aust. J. Basic Appl. Sci. 2015, 5, 56–68. [Google Scholar]
  67. Malik, S.; de Mesquita, L.; Silva, C.; de Mesquita, J.; de Sá Rocha, E.; Bose, J.; Abiri, R.; de Maria Silva Figueiredo, P.; Costa-Júnior, L. Chemical Profile and Biological Activities of Essential Oil from Artemisia vulgaris L. cultivated in Brazil. Pharmaceuticals 2019, 12, 49. [Google Scholar] [CrossRef] [Green Version]
  68. European Food Safety Authority. Botanical Summary Report. Available online: (accessed on 1 March 2020).
  69. Koul, B.; Taak, P. The Artemisia Genus: A Review on Traditional Uses, Phytochemical Constituents, Pharmacological Properties and Germplasm Conservation. J. Glycomics Lipidomics 2017, 7, 142. [Google Scholar] [CrossRef]
  70. Duke, J.A.; Bogenshutz-Godwin, M.J.; DuCellier, M.J.; Duke, P.A. Handbook of Medicinal Herbs, 2nd ed.; CRC Press: Boca Raton, FL, USA, 2002. [Google Scholar]
  71. Pelkonen, O.; Abass, K.; Wiesner, J. Thujone and thujone-containing herbal medicinal and botanical products: Toxicological assessment. Regul. Toxicol. Pharmacol. 2013, 65, 100–107. [Google Scholar] [CrossRef]
  72. European Union Reference Laboratory for Feed Additives. Evaluation Report on the Analytical Methods Submitted in Connection with the Application for Authorisation of a Feed Additive According to Regulation (EC) No 1831/2003. Available online: (accessed on 1 March 2020).
  73. Jerkovic, I.; Mastelic, J.; Milos, M.; Juteau, F.; Masotti, V.; Viano, J. Chemical variability of Artemisia vulgaris L. essential oils originated from the Mediterranean area of France and Croatia. Flavour Fragr. J. 2003, 18, 436–440. [Google Scholar] [CrossRef]
  74. Michaelis, K.; Vostrowsky, O.; Paulini, H.; Zintl, R.; Knobloch, K. On the Essential Oil Components from Blossoms of Artemisia vulgaris L. Z. Naturforsch. C Biosci. 1982, 37, 152–158. [Google Scholar] [CrossRef]
  75. Misra, L.N. σ-Thujone, the major component of the essential oil from Artemisia vulgaris growing wild in Nilgiri hills. J. Nat. Prod. 1986, 3, 941. [Google Scholar] [CrossRef]
  76. Mucciarelli, M.; Caramiello, R.; Maffei, M.; Chialva, F. Essential oils from some Artemisia species growing spontaneously in North-West Italy. Flavour Fragr. J. 1995, 10, 25–32. [Google Scholar] [CrossRef]
  77. Nano, M.G.; Bicchini, C.; Frattini, C.; Gallino, M. On the composition of some oils from Artemisia vulgaris. Plante Med. 1976, 30, 209–215. [Google Scholar] [CrossRef]
  78. Näf-Müller, R.; Pickenhagen, W.; Willhalm, B. New Irregular Monoterpenes in Artemisia vulgaris. Helv. Chim. Acta 1981, 64, 1424–1430. [Google Scholar] [CrossRef]
  79. Dũng, N.X.; Nam, V.V.; Huóng, H.T.; Leclercq, P.A. Chemical composition of the essential oil of Artemisia vulgaris L. var. indica Maxim. from Vietnam. J. Essent. Oil Res. 1992, 4, 433–434. [Google Scholar] [CrossRef]
  80. Thi Phuong Thao, N.; Thi Thuy, N.; Minh Hoi, T.; Huy Thai, T.; Muselli, A.; Bighelli, A.; Castola, V.; Casanova, J. Artemisia vulgaris L. from Vietnam: Chemical variability and composition of the oil along the vegetative life of the plant. J. Essent. Oil Res. 2004, 16, 358–361. [Google Scholar] [CrossRef]
  81. Des Berendes, J. Pedanios Dioskurides aus Anazarbos Arzneimittellehre in fünf Büchern. Übersetzt und mit Erklärungen Versehen; Band III.; Ferdinand Enke: Stuttgart, Germany, 1902. [Google Scholar]
  82. König, R.; Winkler, G.; Plinius Secundus, D.Ä.C. Naturkunde Lateinisch-Deutsch. 37 Bücher (und Register) in 32 Bänden; Artemis & Winkler: München, Germany, 1977. [Google Scholar]
  83. Karl Gottlob Kühn. Claudi Galeni Opera Omnia, Bd. 11, Leipzig 1826; Olms-Verlag: Hildesheim, Germany, 1965. [Google Scholar]
  84. Stoll, U. Das “Lorscher Arzneibuch“ (Codex Bambergis Medicinalis 1); Franz Steiner Verlag: Stuttgart, Germany, 1902. [Google Scholar]
  85. Wölfel, H. Das Arzneidrogenbuch Circa Instans in einer Fassung des XIII. Jahrhunderts aus der Universitätsbibliothek Erlangen: Text. und Kommentar als Beitrag zur Pflanzen- und Drogenkunde des Mittelalters; Mathematisch-Naturwissenschaftliche Dissertation: Berlin, Germany, 1939. [Google Scholar]
  86. Choulant (Hrsg.), J.L. Macer Floridus, De viribus Herbarum ‘una cum Walafridi Strabonis, Othonis Cremonensis et Ioannis Folcz’ ‘Carminibus Similis Argumenti Secundum Codices Manuscriptos et Veteres Editione Srecensuit, Supplevit et Adnotatione Criticains Truxit Ludovicus Choulan’; Leopold Voss: Leipzig, Germany, 1832. [Google Scholar]
  87. Wonnecke von Kaub, J.; Schöffer, P. Gart der Gesundheit, Mainz 1485, Kap. 1; Konrad Kölbl: München, Germany, 1996. [Google Scholar]
  88. Lonitzer, A.; Uffenbach, P. Kräuter-Buch und Künstliche Conterfeyungen der Bäumen, Stauden, Hecken, Kräuter; Verlag Bartholomae: Ulm, Germany, 1703. [Google Scholar]
  89. Brunfels, O. Contrafayt Kreüterbuch (mit naturgetreuen Abb. Hans Weidnitz), 2 Teile.; Basel, Switzerland, 1532.
  90. Madaus, G. Lehrbuch der Biologischen Heilmittel, Band I.; Georg Olms Verlag: Hildesheim, Germany, 1976. [Google Scholar]
  91. Tusaie, K.R.; Fitzpatric, J.J. Advanced Practice Psychiatric Nursing, 2nd ed.; Springer Publishing Company: Berlin, Germany, 2017. [Google Scholar]
  92. Ulbricht, C.E. Natural Standard, Herb and Supplement Guide; An. Evidence-Based Reference; Elsevier: Amsterdam, The Netherlands, 2010. [Google Scholar]
  93. Wheeler, J.; Coppock, B.; Chen, C. Does the burning of moxa (Artemisia vulgaris) in traditional Chinese medicine constitute a health hazard? Acupunct. Med. 2009, 27, 16–20. [Google Scholar] [CrossRef]
  94. Khare, C.P. Indian Herbal Remedies; Springer: Berlin, Germany, 2004. [Google Scholar]
  95. Rodrigues, E. Plants and animals utilized as medicines in the Jaú National Park (JNP), Brazilian Amazon. Phyther. Res. 2006, 20, 378–391. [Google Scholar] [CrossRef]
  96. de Albuquerque, U.P.; Monteiro, J.M.; Ramos, M.A.; de Amorim, E.L.C. Medicinal and magic plants from a public market in northeastern Brazil. J. Ethnopharmacol. 2007, 110, 76–91. [Google Scholar] [CrossRef]
  97. Milliken, W. Malaria and antimalarial plants in Roraima, Brazil. Trop. Doct. 1997, 27 (Suppl. S1), 20–25. [Google Scholar] [CrossRef]
  98. Quisumbing, E. Medicinal Plants of the Philippines; Bureau of Printing: Manila, Philippines, 1978. [Google Scholar]
  99. Lockie, A. Encyclopedia of Homeopathy; DK Publishing: New York, NY, USA, 2006; ISBN 9780756618711. [Google Scholar]
  100. Agence Nationale de sécurité du Médicamen. Mugwort for Homoeopathic Preparations Artemisia vulgaris 2004, 3–5. Available online: (accessed on 15 March 2020).
  101. Oyedemi, S.; Coopoosamy, R. Preliminary Studies on the Antibacterial and Antioxidative Potentials of Hydroalcoholic Extract from the Whole Parts of Artemisia vulgaris L. Int. J. Pharmacol. 2015, 2, 1–9. [Google Scholar] [CrossRef]
  102. Baykan Erel, Ş.; Reznicek, G.; Şenol, S.G.; Karabay Yavaşoğulu, N.Ü.; Konyalioğlu, S. Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia. Turk. J. Biol 2012, 75, 75–84. [Google Scholar] [CrossRef]
  103. Ben Nasr, S.; Aazza, S.; Mnif, W.; Miguel, M. In-vitro antioxidant and anti-inflamatory activities of Pituranthos chloranthus and Artemisia vulgaris from Tunisia. Int. J. Appl. Pharm. Sci. Res. 2020, 11, 605–614. [Google Scholar] [CrossRef]
  104. El-Tantawy, W.H. Biochemical effects, hypolipidemic and anti-inflammatory activities of Artemisia vulgaris extract in hypercholesterolemic rats. J. Clin. Biochem. Nutr. 2015, 57, 33–38. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  105. Gilani, A.H.; Yaeesh, S.; Jamal, Q.; Ghayur, M.N. Hepatoprotective activity of aqueous-methanol extract of Artemisia vulgaris. Phyther. Res. 2005, 19, 170–172. [Google Scholar] [CrossRef] [PubMed]
  106. Khan, A.U.; Gilani, A.H. Antispasmodic and bronchodilator activities of Artemisia vulgaris are mediated through dual blockade of muscarinic receptors and calcium influx. J. Ethnopharmacol. 2009, 126, 480–486. [Google Scholar] [CrossRef]
  107. Lee, S.-J.; Chung, H.-Y.; Lee, I.-K.; Oh, S.-U.; Yoo, I.-D. Phenolics with Inhibitory Activity on Mouse Brain Monoamine Oxidase (MAO) from Whole Parts of Artemisia vulgaris L. (Mugwort). Food Sci. Biotechnol. 2000, 9, 179–182. [Google Scholar]
  108. Yang, X.; Xiong, X.; Yang, G.; Wang, J. Effectiveness of Stimulation of Acupoint KI 1 by Artemisia vulgaris (Moxa) for the Treatment of Essential Hypertension: A Systematic Review of Randomized Controlled Trials. Evid. Based Complement Altern. Med. 2014, 2014, 187484. [Google Scholar] [CrossRef] [Green Version]
  109. Shaik, A.; Kanhere, R.S.; Cuddapah, R.; Nelson, K.S.; Vara, P.R.; Sibyala, S. Antifertility activity of Artemisia vulgaris leaves on female Wistar rats. Chin. J. Nat. Med. 2014, 12, 180–185. [Google Scholar] [CrossRef]
  110. Jakovljević, M.R.; Grujičić, D.; Vukajlović, J.T.; Marković, A.; Milutinović, M.; Stanković, M.; Vuković, N.; Vukić, M.; Milošević-Djordjević, O. In vitro study of genotoxic and cytotoxic activities of methanol extracts of Artemisia vulgaris L. and Artemisia alba Turra. South Afr. J. Bot. 2020, 132, 117–126. [Google Scholar] [CrossRef]
  111. Saleh, A.M.; Aljada, A.; Rizvi, S.A.A.; Nasr, A.; Alaskar, A.S.; Williams, J.D. In vitro cytotoxicity of Artemisia vulgaris L. essential oil is mediated by a mitochondria-dependent apoptosis in HL-60 leukemic cell line. BMC Complement. Altern. Med. 2014, 14, 226. [Google Scholar] [CrossRef] [Green Version]
  112. Raj Singh, B.; Singh, V.; Karan Singh, R.; Toppo, S.; Haque, N.; Ebibeni, N. Antimicrobial effect of Artemisia vulgaris essential oil. Nat. Prod. Indian J. 2011, 5, 1–7. [Google Scholar]
  113. Hiremath, S.K.; Kolume, D.G.; Muddapur, U.M. Antimicrobial activity of Artemisia vulgaris Linn. (Damanaka). Int. J. Res. Ayurveda Pharm. 2011, 2, 1674–1675. [Google Scholar]
  114. Singh, R.; Verma, P.; Singh, G. Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium. J. Intercult. Ethnopharmacol. 2012, 1, 101. [Google Scholar] [CrossRef]
  115. Afsar, S.K.; Rajesh Kumar, K.; Venu Gopal, J.; Raveesha, P. Assessment of anti-inflammatory activity of Artemisia vulgaris leaves by cotton pellet granuloma method in Wistar albino rats. J. Pharm. Res. 2013, 7, 463–467. [Google Scholar] [CrossRef]
  116. Olsen, O.T.; Frolund, L.; Heinig, J.; Jacobsen, L.; Svendsen, U.G. A double-blind, randomized study investigating the efficacy and specificity of immunotherapy with Artemisia vulgaris or Phleum pratense/Betula verrucosa. Allergol. Immunopathol. (Madr). 1995, 23, 73–78. [Google Scholar]
  117. Kodippili, K.; Ratnasooriya, W.D.; Premakumara, S.; Udagama, P.V. An investigation of the antimalarial activity of Artemisia vulgaris leaf extract in a rodent malaria model. Int. J. Green Pharm. 2011, 5, 1–7. [Google Scholar] [CrossRef]
  118. Bamunuarachchi, G.S.; Ratnasooriya, W.D.; Premakumara, S.; Udagama, P.V. Antimalarial properties of Artemisia vulgaris L. ethanolic leaf extract in a Plasmodium berghei murine malaria model. J. Vector Borne Dis. 2013, 50, 278–284. [Google Scholar]
  119. Caner, A.; Döşkaya, M.; Deǧirmenci, A.; Can, H.; Baykan, Ş.; Üner, A.; Başdemir, G.; Zeybek, U.; Gürüz, Y. Comparison of the effects of Artemisia vulgaris and Artemisia absinthium growing in western Anatolia against trichinellosis (Trichinella spiralis) in rats. Exp. Parasitol. 2008, 119, 173–179. [Google Scholar] [CrossRef]
  120. Teixeira, B.; Marques, A.; Ramos, C.; Neng, N.R.; Nogueira, J.M.F.; Saraiva, J.A.; Nunes, M.L. Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Ind. Crops Prod. 2013, 43, 587–595. [Google Scholar] [CrossRef]
  121. Singh, B.R.; Singh, V.; Singh, R.K.; Toppo, S.; Haque, N.; Ebibeni, N. Comparative evaluation of antimicrobial effect of Artemisia vulgaris essential oils extracted from fresh and dried herb. Med. Plants 2012, 4, 76–82. [Google Scholar] [CrossRef]
  122. Lavor, P.L.; Santiago, G.M.P.; Da Silva Gois, R.W.; De Sousa, L.M.; Bezerra, G.D.P.; Romero, N.R.; Arriaga, Â.M.C.; Lemos, T.L.G.; Alves, P.B.; Gomes, P.C.S. Larvicidal activity against Aedes aegypti of essential oils from northeast Brazil. Nat. Prod. Commun. 2012, 7, 1391–1392. [Google Scholar] [CrossRef] [Green Version]
  123. Balasubramani, S.; Sabapathi, G.; Moola, A.K.; Solomon, R.V.; Venuvanalingam, P.; Bollipo Diana, R.K. Evaluation of the Leaf Essential Oil from Artemisia vulgaris and Its Larvicidal and Repellent Activity against Dengue Fever Vector Aedes aegypti-An Experimental and Molecular Docking Investigation. ACS Omega 2018, 3, 15657–15665. [Google Scholar] [CrossRef] [Green Version]
  124. Ilahi, I.; Ullah, F. Larvicidal Activities of Different Parts of Artemisia vulgaris Linn. against Culex quinquefasciatus Say. (Diptera: Culicidae). Int. J. Innov. Appl. Stud. 2013, 2, 189–195. [Google Scholar]
  125. Sharifian, I.; Hashemi, S.M.; Darvishzadeh, A. Fumigant toxicity of essential oil of Mugwort (Artemisia vulgaris L.) against three major stored product beetles. Arch. Phytopathol. Plant. Prot. 2013, 46, 445–450. [Google Scholar] [CrossRef]
  126. Wang, J.; Zhu, F.; Zhou, X.M.; Niu, C.Y.; Lei, C.L. Repellent and fumigant activity of essential oil from Artemisia vulgaris to Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). J. Stored Prod. Res. 2006, 42, 339–347. [Google Scholar] [CrossRef]
  127. Seul Park, Y.; He Nam, G.; Jo Jo, K.; Won Kawk, H.; Yoo, J.-G.; Dong Jang, J.; Moon Kang, S.; Yong Kim, S.; Min Kim, Y. Adequacy of the Anti-aging and Anti-wrinkle Effects of the Artemisia vulgaris Fermented Solvent Fraction. Korean Soc. Biotechnol. Bioeng. J. 2019, 34, 199–206. [Google Scholar] [CrossRef]
  128. Bampidis, V.; Azimonti, G.; de Lourdes Bastos, M.; Christensen, H.; Kouba, M.; Kos Durjava, M.; López-Alonso, M.; López Puente, S.; Marcon, F.; Mayo, B.; et al. Safety and efficacy of a tincture derived from Artemisia vulgaris L. (Mugwort tincture) when used as a sensory additive in feed for all animal species. EFSA J. 2019, 17, 5879. [Google Scholar] [CrossRef] [Green Version]
  129. European Food Safety Authority. EFSA Supporting Publications. 2014, Volume 11. Available online: (accessed on 1 April 2020).
  130. Food and Drug Administration Pollens-Weeds and Garden Plants 2011. Available online: (accessed on 17 March 2020).
  131. Ipsen, H.; Formgren, H.; Løswenstein, H.; Ingemann, L. Immunochemical and Biological Characterization of a Mugwort (Artemisia vulgaris) Pollen Extract. Allergy 1985, 40, 289–294. [Google Scholar] [CrossRef]
  132. Yong, W.L.; Soo, Y.C.; Eun, K.L.; Jung, H.S.; Park, J.W.; Hong, C.S. Cross-allergenicity of pollens from the Compositae family: Artemisia vulgaris, Dendranthema grandiflorum, and Taraxacum officinale. Ann. Allergy Asthma Immunol. 2007, 99, 526–533. [Google Scholar] [CrossRef]
  133. Wrangsjö, K.; Ros, A.M.; Wahlberg, J.E. Contact allergy to Compositae plants in patients with summer-exacerbated dermatitis. Contact Dermatitis 1990, 22, 148–154. [Google Scholar] [CrossRef] [PubMed]
  134. Haw, S.; Cho, H.-R.; Lee, M.-H. Allergic contact dermatitis associated with mugwort (Artemisia vulgaris). Contact Dermatitis 2010, 62, 61–63. [Google Scholar] [CrossRef] [PubMed]
  135. European Food Safety Authority Compendium of botanicals reported to contain naturally occuring substances of possible concern for human health when used in food and food supplements. EFSA J. 2012, 10. [CrossRef] [Green Version]
  136. Journal, T.E. Opinion of the Scientific Panel on Food Additives, Flavourings, Processing Aids and Materials in Contact with Food (AFC) on hydrocyanic acid in flavourings and other food ingredients with flavouring properties. EFSA J. 2004, 2, 1–28. [Google Scholar] [CrossRef]
  137. Sudarshana, M.S. In vitro regeneration of multiple shoots from encapsulated somatic embryos of Artemisia vulgaris L. IOSR J. Pharm. Biol. Sci. 2013, 6, 11–15. [Google Scholar] [CrossRef]
Figure 1. The chemical structure of sesquiterpenoid lactones characteristic of A. vulgaris.
Figure 1. The chemical structure of sesquiterpenoid lactones characteristic of A. vulgaris.
Molecules 25 04415 g001
Figure 2. The chemical structure of volatile compounds characteristic of the essential oil of A. vulgaris herb.
Figure 2. The chemical structure of volatile compounds characteristic of the essential oil of A. vulgaris herb.
Molecules 25 04415 g002aMolecules 25 04415 g002b
Figure 3. Chemical structure of the tricyclic sesquiterpenoids characteristic of the root of A. vulgaris.
Figure 3. Chemical structure of the tricyclic sesquiterpenoids characteristic of the root of A. vulgaris.
Molecules 25 04415 g003
Figure 4. Chemical structure of prunasin—a cyanogenic glycoside isolated from A. vulgaris.
Figure 4. Chemical structure of prunasin—a cyanogenic glycoside isolated from A. vulgaris.
Molecules 25 04415 g004
Table 1. The Latin synonymous names of A. vulgaris L.
Table 1. The Latin synonymous names of A. vulgaris L.
Artemisia vulgaris Burm.f. [Illegitimate]
Artemisia vulgaris Mattf. [Illegitimate]
Artemisia vulgaris C.B.Clarke
Artemisia vulgaris var. americana Besser
Artemisia vulgaris f. angustisecta Fiori
Artemisia vulgaris var. aromatica Sacc.
Artemisia vulgaris subsp. candicans (Rydb.) H.M.Hall & Clem.
Artemisia vulgaris var. candicans (Rydb.) M.Peck
Artemisia vulgaris subsp. coarctata (Fors ex Besser) Ameljcz.
Artemisia vulgaris var. coarctica Besser
Artemisia vulgaris var. douglasiana H.St.John
Artemisia vulgaris subsp. flodmanii (Rydb.) H.M.Hall & Clem.
Artemisia vulgaris var. flodmanii (Rydb.) M.Peck
Artemisia vulgaris var. gilvescens (Miq.) Nakai
Artemisia vulgaris var. glabra Ledeb.
Artemisia vulgaris var. glandulifera (L.F.Hend.) M.Peck
Artemisia vulgaris subsp. gnaphalodes (Nutt.) H.M.Hall & Clem.
Artemisia vulgaris var. gnaphalodes (Nutt.) Kuntze
Artemisia vulgaris subsp. heterophylla (Nutt.) H.M.Hall & Clem.
Artemisia vulgaris var. incana Maxim.
Artemisia vulgaris var. incanescens Franch.
Artemisia vulgaris var. incompta (Nutt.) H.St.John
Artemisia vulgaris var. indica (Willd.) Hassk.
Artemisia vulgaris var. kamtschatica Besser
Artemisia vulgaris var. kiusiana Makino
Artemisia vulgaris var. latiloba Ledeb.
Artemisia vulgaris var. leucophylla Turcz. ex Besser
Artemisia vulgaris var. littoralis Suksd.
Artemisia vulgaris var. longifolia (Nutt.) M.Peck
Artemisia vulgaris subsp. longifolia (Nutt.) H.M.Hall & Clem.
Artemisia vulgaris subsp. ludoviciana (Nutt.) H.M.Hall & Clem.
Artemisia vulgaris var. ludoviciana (Nutt.) Kuntze
Artemisia vulgaris var. maximowiczii Nakai
Artemisia vulgaris var. mexicana (Willd. ex Spreng.) Torr. & A.Gray
Artemisia vulgaris subsp. mexicana (Willd. ex Spreng.) H.M.Hall & Clem.
Artemisia vulgaris subsp. michauxiana (Besser) H.St.John
Artemisia vulgaris var. minor Ledeb.
Artemisia vulgaris var. mongolica Fisch. ex Besser
Artemisia vulgaris f. montana Nakai
Artemisia vulgaris var. nilagirica C.B.Clarke
Artemisia vulgaris var. nipponica Nakai
Artemisia vulgaris f. nipponica Nakai
Artemisia vulgaris subsp. redolens (A.Gray) H.M.Hall & Clem.
Artemisia vulgaris var. rubriflora Turcz. ex Besser
Artemisia vulgaris subsp. selengensis Thell.
Artemisia vulgaris var. selengensis (Turcz. ex Besser) Maxim.
Artemisia vulgaris subsp. serrata (Nutt.) H.M.Hall & Clem.
Artemisia vulgaris var. stolonifera Maxim.
Artemisia vulgaris var. suaveolens Bég.
Artemisia vulgaris var. tenuifolia Turcz. ex DC.
Artemisia vulgaris subsp. tilesii (Ledeb.) H.M.Hall & Clem.
Artemisia vulgaris var. umbrosa Turcz. ex Besser
Artemisia vulgaris subsp. urjanchaica Ameljcz.
Artemisia vulgaris var. verbenacea Kom.
Artemisia vulgaris var. viridissima Kom.
Artemisia vulgaris var. vulgaris
Artemisia vulgaris var. wrightii (A.Gray) H.M.Hall & Clem.
Artemisia vulgaris subsp. wrightii (A.Gray) H.M.Hall & Clem.
Table 2. The chemical composition of A. vulgaris aerial parts.
Table 2. The chemical composition of A. vulgaris aerial parts.
Chemical GroupCompoundContentReferences
Sesquiterpenoid lactones1,2,3,4-diepoxy-11(13)-eudesmen-
12,8-olide; yomogin
na *[10]
psilostachyin, psilostachyin Cna[3,54]
vulgarin0.15% d.w.[3,30,55]
artemisinin0–2.3% dw.[56,57]
Flavonoidsquercetin 3-galactoside,
quercetin 3-glucoside,
kaempferol 3-glucoside,
kaempferol 3-rhamnoside,
kaempferol 3-rutinoside,
kaempferol 7-glucoside,
luteolin 7-glucoside
~6 mg/kg d.w.
~11 mg/kg d.w.
~10 mg/kg d.w.
~5 mg/kg d.w.
~5 mg/kg d.w.
~5 mg/kg d.w.
~5 mg/kg d.w.
apigenin~6 mg/kg d.w.[3,11,13,58]
chrysoeriol~2.5 mg/kg d.w.[13]
~40 mg/kg d.w.
~5 mg/kg d.w.
~2.5 mg/kg d.w.
~5 mg/kg d.w.
~10 mg/kg d.w.
hyperoside0.5 mg/g d.w.[3,58,59]
jaceosidin~3 mg/kg d.w.[13]
quercetin~3 mg/kg d.w.[3,11,30,58]
luteolin~40 mg/kg d.w.[3,13,58]
rutoside~7–20 mg/kg d.w.[11,13,59]
tricine,~3 mg/kg d.w.[13]
vitexin~4 mg/kg d.w.[3,58]
Coumarinsesculin, esculetin, umbelliferonena[3,60]
Phenolic acids1,5-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid0.3% d.w.
0.2% d.w.
5-feruloylquinic acid
quinic acid
protocatechuic acid glucoside
3-O-caffeoylquinic acid
5-O-caffeoylquinic acid
4,5-O-di-caffeoylquinic acid
0.37 mg/g d.w.
1.5 mg/g d.w.
3.2 mg/g d.w.
0.44 mg/g d.w.
2.8 mg/g d.w.
11 mg/g d.w.
caffeic acidna[3,11,61]
Sterolssitosterol, stigmasterol [3]
Fatty acidsna13.3 mg/g f.w.[46]
Cyanogenic glycosidesprunasinna[3,63]
Vitaminsascorbic acidna[30]
* na—no data available.
Table 3. The chemical composition of essential oil from A. vulgaris aerial parts.
Table 3. The chemical composition of essential oil from A. vulgaris aerial parts.
Chemical Groups/CompoundsEstimated Content (%)References
artemisia alcohol, artemisyl acetate, isobornyl acetate, (Z)-β-ocymen, terpinolene0–2.6[62]
artemisia ketone0–2.89[62,64,65]
bornyl acetate0–6.29[15,16,17,65,67]
trans-carveol, trans-pinocarveol0–0.77[15,64,65]
cis-chrysanthenol, dehydrosabinaketone, methyleugenol, verbenyl acetate, p-cymene-8-ol, piperitone, p-mentha-1,4-dien-7-ol, sabinaketone, trans-verbenol, cuminol0–7.0[15]
isobornyl 2-methylbutyrate, menthol0–5[46]
3-thujanol [17,64]
chrysanthenyl acetate0–23.6[15,46,62]
cis-sabinene hydrate,0–1.08[62,64,65]
trans-sabinene hydrate0–0.55[15,62,64,65]
santolina triene0–0.6[15,16,17,62,64]
α-fenchen [16,17,64]
caryophyllene oxide1.52–5.5[3,15,16,17,46,62,64,65,66]
trans-caryophyllene, trans-salvene2.5–12.2[46,65]
caryophylla-4(14),8(15)-diene-5-α-ol, (E)-nerolidol, humulene epoxide II, germacrene d-4-ol, ledol, farnesyl acetate, lanceol acetate, salvial-4(14)-en-1-one, silphiperfol-5-en-3-ol (Z)-
β-farnesene, α-calacorene, β-chamigrene,
davanone, silphiperfol-4,7(14)-diene,
β-elemene0–8[17], [64]
α-elemene, β-bisabolene0–8.8[16,62]
germacrene D5.3–15.1[15,46,62,64]
7-α-silphiperfol-5-ene, epi-β-santalene, modhephene, petasitene, presilphiperfol-7-ene, silphin-1-ene, valeranone, humulene oxide,
α-bisabololene, α-cedrene,
α-isocomene, α-trans-bergamotene
Table 4. Differences in the composition of essential oils from the herb of A. vulgaris of various origins.
Table 4. Differences in the composition of essential oils from the herb of A. vulgaris of various origins.
Origin of PlantsMain ComponentsReferences
Brazilcaryophyllene (37.45%)
germacrene D (16.17%)
humulene (13.66%)
borneol (6.80%)
caryophyllene oxide (5.67%)
Francecamphor (1–13%)
1,8-cineole (1–23%)
terpinen-4-ol (1–19%)
Germanysabinene (16%)
myrcene (14%)
1,8-cineole (10%)
Indiaα-thujone and thujone isomer (β-thujone)
Italycamphor (47%)[76]
camphor (2–20%)
myrcene (9–70%)
1,8-cineole (1–27%)
borneol (3–18%)
Lithuania (North part)sabinene (0–8.4%)
β-pinene (0.1–12.9%)
1,8-cineole (2.6–17.6%)
cis-thujone (0–12.9%)
trans-thujone (0–20.2%)
chrysanthenyl acetate (0–23.6%)
caryophyllene (2.5–12.2%)
germacrene D (5.3–15.1%)
Vietnamβ-caryophyllene (24%)
β-cubebene (12%)
β-elemene (6%)
Table 5. Pharmacological properties of A. vulgaris herb and root extract.
Table 5. Pharmacological properties of A. vulgaris herb and root extract.
Activity *InformationCompounds Supposed to be ResponsibleReferences
AntioxidantProved by different methods: DPPH, lipid peroxidation, protein glycation, xanthine oxidases, ABTS, hydroxyl superoxide, nitric oxide, ferric reducing power activity and inhibition of lipid peroxidation by thiobarbituric acid reactive species assays.
Increasing the level of ascorbic acid and glutathione.
flavonoids, flavonols, phenolic acids[8,59,101,102,103]
HypolipemicNormalized serum lipid profile, a significant increase in paraoxonase-1 activity and decrease in serum malondialdehyde, nitric oxide, tumor necrosis factor-α level and decrease in hydroxymethylglutaryl-CoA reductase activity.
Lowering total cholesterol, triglycerides, LDL, VLDL, and increasing HDL and atherogenicity indicator (aqueous extract of A. vulgaris roots) *.
HepatoprotectiveProphylactic protective effect limiting inflammation, cellular oedema, apoptotic cell count, and hyperaemia of the hepatic parenchyma. [105]
AntispasmolyticAntagonism towards H1 histamine receptors. [10,11]
BronchodilatoryAnticholinergic and Ca2+ antagonist mechanisms. Histamine H1 antagonism in the ileum and trachea.yomogin (sesquiterpene lactone), alkaloids, coumarins, flavonoids, saponins, sterols, tannins, terpenoids[10,106]
AnalgesicMild peripheral anti-nociceptive effect. probably induced by rutoside, hydroxybenzoic acid derivatives, and caffeic acid and its derivatives[11]
MAO inhibitionInhibition of mouse brain monoamine oxidase (MAO) enzyme.flavonoids: jaceosidine, eupafolin, luteolin, quercetin, apigenine; coumarins: esculetin, esculetin-6-methylether, scopoletin[107]
AntihypertensiveInhibiting the hypertensive effect of noradrenaline.
Moxibustion showed lowering the blood pressure compared to antihypertensive drugs by stimulation of acupoint KI 1.
na **
Moxibustion—a traditional Chinese method that uses the heat generated by burning herbal preparations containing A. vulgaris to stimulate acupuncture points
EstrogenicAntagonism towards the estrogen receptor and activation of gene transcription. Induction of gene transcription by eriodictyol and apigenin.
Anti-implantation activity and estrogenic activity on female Wistar rats.
CytotoxicInhibition of tumour cell growth in cancer cell lines: MCF7, HeLa, A7R5, 293T, HL-60 and SW-480.phenolic compounds, flavonoids, essential oil[14,110,111]
Antifungal and antibacterialInhibitory effect of the oil fraction on the development of Candida albicans.
Inhibitory effect of the oil fraction on the development of Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus mutans, Candida albicans, and Aspergillus niger.
probably associated with the presence of essential oils, 1,8-cineole, α-thujone and camphene[15,16,67,102,112,113,114]
Anti-inflammatoryNormalization of serum lipid profile, increase in paraoxonase-1 activity and decrease in serum malondialdehyde, nitric oxide and tumor necrosis factor-α level. Proved by lipoxygenase (LOX) inhibitory activity assay and “Cotton Pellet Granuloma Method”.na[103,104,115]
AntialergenicDecrease in skin sensitivity and eye[116]
AntimalarialActivity angainst Plasmodium yoelii and P.[117,118]
AnthelminticActivity against Trichinella[119]
* all of the listed activities have been proved for extracts of A. vulgaris herb, except for hypolipemic effects; ** na—no data available.
Table 6. Insecticidal activity of different raw materials from A. vulgaris—examples.
Table 6. Insecticidal activity of different raw materials from A. vulgaris—examples.
Insect SpeciesPathogenityRaw Material of A. VulgarisReferences
Aedes aegypti (Egyptian mosquito)denge fever
virus vector
essential oil from the leaves[122,123]
essential oil from the herb[17]
nanoparticles with leaf extract[123]
Culex quinquefasciatus Say. (Culex fatigans) (arbo virus)vector of avian malaria, vector of wuchereria bancroftileaves extracts[124]
stem extracts
root extracts
Tribolium castaneum (Herbst)stored–product insect pestsessential oil from leaves[126]
essential oil from aerial parts[125]
Callosobruchus maculatus (F.)stored–product beetlesessential oil from aerial parts[125]
Rhyzopertha dominicastored–product beetlesessential oil from aerial parts[125]
Table 7. Applications of A. vulgaris in cosmetology as recommended by the CosIng database.
Table 7. Applications of A. vulgaris in cosmetology as recommended by the CosIng database.
Name in CosIng DatabaseFunctions
Artemisia vulgaris extractskin conditioning
Artemisia vulgaris herb extractperfuming
Artemisia vulgaris oilperfuming, skin conditioning
Bacillus/ Artemisia vulgaris extract/ferment filtrateskin conditioning
hydrolyzed Artemisia vulgaris leafhumectant, skin conditioning
Lactobacillus/Chrysanthemum zawadskii flower/Gleditsia japonica fruit/Thuja orientalis leaf/Morus alba bark/Panax ginseng root/Artemisia vulgaris extract ferment filtrateskin conditioning
Bacillus/Cinnamomum cassia bark/Momordica charantia/Opuntia humifusa fruit/Aloevera/Artemisia vulgaris/Camellia sinensis/Nelum bonucifera leaf/Pueraria thunbergiana root ferment filtrateantioxidant, skin protecting
Saccharomyces/Aloe barbadensis leaf/Artemisia vulgaris leaf/Prunus mume fruit ferment filtrateskin conditioning

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Ekiert, H.; Pajor, J.; Klin, P.; Rzepiela, A.; Ślesak, H.; Szopa, A. Significance of Artemisia Vulgaris L. (Common Mugwort) in the History of Medicine and Its Possible Contemporary Applications Substantiated by Phytochemical and Pharmacological Studies. Molecules 2020, 25, 4415.

AMA Style

Ekiert H, Pajor J, Klin P, Rzepiela A, Ślesak H, Szopa A. Significance of Artemisia Vulgaris L. (Common Mugwort) in the History of Medicine and Its Possible Contemporary Applications Substantiated by Phytochemical and Pharmacological Studies. Molecules. 2020; 25(19):4415.

Chicago/Turabian Style

Ekiert, Halina, Joanna Pajor, Paweł Klin, Agnieszka Rzepiela, Halina Ślesak, and Agnieszka Szopa. 2020. "Significance of Artemisia Vulgaris L. (Common Mugwort) in the History of Medicine and Its Possible Contemporary Applications Substantiated by Phytochemical and Pharmacological Studies" Molecules 25, no. 19: 4415.

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