The Artemisia L. Genus: A Review of Bioactive Essential Oils

Numerous members of the Anthemideae tribe are important as cut flowers and ornamental crops, as well as being medicinal and aromatic plants, many of which produce essential oils used in folk and modern medicine and in the cosmetics and pharmaceutical industry. Essential oils generally have a broad spectrum of bioactivity, owing to the presence of several active ingredients that work through various modes of action. Due to their mode of extraction, mostly by distillation from aromatic plants, they contain a variety of volatile molecules such as terpenes, phenol-derived aromatic and aliphatic components. The large genus Artemisia L., from the tribe Anthemideae, comprises important medicinal plants which are currently the subject of phytochemical attention due to their biological and chemical diversity. Artemisia species, widespread throughout the world, are one of the most popular plants in Chinese traditional preparations and are frequently used for the treatment of diseases such as malaria, hepatitis, cancer, inflammation and infections by fungi, bacteria and viruses. Extensive studies of the chemical components of Artemisia have led to the identification of many compounds as well as essentials oils. This review summarizes some of the main reports on the chemistry and anti-infective activities of Artemisia. Li. essential oils from the data in the recent literature (2000–2011).


Introduction
Medicinal plants are Nature's gift to human beings to help them pursue a disease-free healthy life, and thus can play an important role in preserving health. Plants have been used as drugs by humans since thousands of years ago. Today, all the world's cultures have an extensive knowledge of herbal medicine. Traditional medicine is based on beliefs and practices that existed before the development of so-called "modern medicine" or "scientific drug therapy". These practices are part of a country's cultural heritage and are transmitted orally or by written transmission.
Artemisia echegaray Hieron is commonly known in Argentina as "ajenjo", and is used as a natural food additive [13].
Decoctions of leaves and stems of Artemisia frigida Willd. are used for coughs and diabetes [4]. Artemisia fukudo Makino is distributed along the shorelines of South Korea's Jeju Island and in the south of the Korean Peninsula, Japan and Taiwan. This plant is used as a flavouring agent and in a variety of cosmetics in Korea. It also has various biological effects, including anti-inflammatory, antitumour and antibacterial properties [16].
Artemisia haussknechtii Boiss. is used in food as a flavouring, in perfumes and in pharmaceutical products (for its functional properties) in Iran [17].
Artemisia herba-alba Asso (syn. Artemisia maritima L., Artemisia brevifolia Wall.) is used in the traditional medicine of the northern Badia region of Jordan, in the form of a decoction, against fever and menstrual and nervous problems [18].
Artemisia iwayomogi Kitamura is a perennial herb easily found around Korea. It is called "hanin-jin" or "dowijigi" in Korean, and is traditionally used for the treatment of various liver diseases, including hepatitis [19].
Artemisia judaica L. is a perennial fragrant shrub which grows widely in the deserts and on the Sinai Peninsula in Egypt, and is a very common anthelmintic drug in most North African and Middle-Eastern countries where it is known by the Arabic name of "shih" [7].
The inhabitants of north-eastern of Mexico use an infusion of leaves from Artemisia ludoviciana Nutt. as an antidiarrheal remedy [20].
Artemisia nilagirica (Clarke) Pamp, commonly called "Indian wormwood", is widely found in the hilly areas of India, where it is used as insecticide [21].
Artemisia princeps Willd. ("Japanese mugwort" or "yomogi") is the best known Artemisia in Japan, where it is a fundamental ingredient of the Japanese confection "kusa-mochi". This plant has also been used in traditional Asian medicine for the treatment of inflammation, diarrhoea and many circulatory disorders [19].
Artemisia rubripes Nakai has been used as a traditional Korean medicine for stomach ache, vomiting, diarrhoea and as a haemostatic agent [16].
Artemisia scoparia Waldst. & Kit. ("redstem wormwood") is a faintly scented annual herb which is widespread and common throughout the world, particularly in southwest Asia and central Europe. The success of A. scoparia may be attributed to the presence of phytotoxins, the volatile essential oils, in addition to other non-volatile secondary products. It has been established that aerial parts of A. scoparia yield a volatile essential oil that has medicinal value. It possesses insecticidal, antibacterial, anticholesterolemic, antipyretic, antiseptic, cholagogue, diuretic, purgative and vasodilatatory activity, and is also used for the treatment of gall bladder inflammation, hepatitis and jaundice [4].
Artemisia spicigera C. Koch, known locally in Turkey as "yavsani", is widespread in central and eastern Anatolia, and is traditionally used for skin diseases and ulcerative sores [22].
"Big sagebrush" (Artemisia tridentata Nutt.) is one of the most widely distributed and ecologically important shrub species in western North America. This species serves as a critical habitat and food resource for many animals and invertebrates [13].
Artemisia vulgaris L., commonly known as "mugwort", is a perennial weed growing wild native in Asia, Europe and North America. The plant is widely used in the Philippines, where it is locally known as "herbaka", for its antihypertensive actions. It has also been suggested to have other medicinal activities such as anti-inflammatory, antispasmodic, carminative and anthelmintic properties, and has been used in the treatment of painful menstruation (dysmenorrhoea) and in the induction of labour or miscarriage [23].

Taxonomy
The genus Artemisia is characterized by a wide range of morphological and phytochemical variability, which is associated with different geographical origins of the samples. The genus displays a huge ecological plasticity, with species occurring from sea level to high mountains and from arid zones to wetlands. Additionally, polyploidy is notably common and reported cytotypes differ in external morphology, anatomy, fertility and phytochemical cytogenetically [24].

Chemical Composition of Essential Oils from the Artemisia Genus
Essential oils are volatile, natural, complex compounds characterized by a strong odour and are formed by aromatic plants as secondary metabolites. They are usually obtained by steam or hydro-distillation, although there are several methods for extracting them. These may include the use of liquid carbon dioxide and microwaves, but mainly involve low or high pressure distillation employing boiling water or hot steam.
In nature, essential oils play an important role in the protection of plants as antibacterials, antivirals, antifungals, insecticides and also against herbivores by reducing their appetite for such plants. They also may attract some insects, thereby favouring the dispersion of pollens and seeds, or repel other undesirable insects.
Chemically, essential oils are very complex natural mixtures which can contain about 20-60 components at quite different concentrations. They are characterized by 2-3 major components at fairly high concentrations (20-70%), compared to other components present in trace amounts. Generally, these major components determine the biological properties of the essential oil. The components include two groups with different biosynthetical origins: the main group is composed of terpenes, and the other of aromatic and aliphatic constituents, all characterized by their low molecular weight.
The strong and aromatic smell of some species of Artemisia genus is due mainly to high concentrations of volatile terpenes, constituents of their essential oils, especially in leaves and flowers. The chemical composition of essential oils from the Artemisia genus has been extensively studied in several species from around the world. Many studies have shown that Artemisia species display significant intraspecific variations in the terpene constituents of their essential oils. In some cases, the variation in the volatile components of these plants may occur during plant ontogeny or growth at different altitudes. The quality and yield of essential oils from Artemisia species is influenced by the harvesting season, fertilizer and pH of soils, the choice and stage of drying conditions, the geographic location, chemotype or subspecies, choice of plant part or genotype, or extraction method. Reported constituents of Artemisia species are outlined in Table 1.     Oils from A. nilagirica, a plant growing at different altitudes in Himachal Pradesh, India, were hydrodistilled and analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) [25]. The main constituents of the oil show variation with changes in altitude. At lower, middle and higher altitudes, the major constituents were caryophyllene oxide (28.6%), borneol (35.8%) ( Figure 1) and camphor (46.9%), respectively. The characteristic compounds observed in plants from lower altitudes were 2-hexene-1-ol, β-thujone ( Figure 2), thujanol, myrtenol and lynalyl acetate, while the higher altitude plants were characterized by the presence of α-pinene, β-pinene, limonene ( Figure 3), linalool, γ-gurijunene, germacrane D and farnesol. Padalia et al. [26] analyzed and compared by capillary GC and GC/MS the essential oil yield and composition of the aerial parts of A. annua growing in Uttarakhand, India, at different stages of development. The analyses led to the identification of 81 constituents, forming 91-97.1% of the essential oil composition. However, the essential oil content was found to vary from 0.3% to 0.7% at different stages of growth. The major constituents were camphor (22.8-42.6%), 1,8-cineole (3.7-8.4%) ( Figure 4), linalool (0.1-11.9%), β-caryophyllene (2-9.2%), (E)-β-farnese (1.3-8.5%), germacrene D (0.5-7.3%) and 1-epi-cubenol (0.7-5.2%). With this species cultivated in an experimental greenhouse at the Institute of Botany, Chinese Academy of Sciences, the analysis of their essential oil was also performed by comprehensive two-dimensional GC time-of-flight mass spectrometry (MS) [27]. Viuda-Martos et al. [28] investigated the chemical composition of this species, A. annua, cultivated in Egypt, and 29 components were identified, representing 93.7% of the total oil. The main constituents were 1,8-cineole (8.1%) ( Figure 4) and artemisia ketone (14%) ( Figure 5).  [29] investigated the essential oil composition. GC and GC/MS analysis showed the dominant presence of phenyl alkynes (61.2-85.5%), γ-terpinene (11.1%), p-cymene (4.5%) and (E)-β-ocimene (4.4%). The essential oil from the aerial parts of this species, A. scoparia, from Tajikistan was obtained by hydrodistillation and analyzed by GC/MS [30]. A total of 32 compounds were identified, representing 98% of the total oil composition. A. scoparia oil was dominated by the diacetylenes 1-phenyl-2,4-pentadiyne (34.2%) and capillene (4.9%). Other major components were β-pinene (21.3%), methyl eugenol (5.5%), α-pinene (5.4%), myrcene (5.2%), limonene (5%) ( Figure 3) and β-ocimene (3.8%). The essential oil from the freshly plucked leaves of this species, A. scoparia, growing in wastelands around Chandigah, was extracted by hydrodistillation and analyzed by GC/MS [31]. Previously, the young and mature leaves were separated. GC/Ms analyses revealed a monoterpenoid nature (64-67%), with 44 and 31 constituents in young and mature leaves oil, respectively; β-myrcene (24.1%) and p-cymene (27%) were the major constituents in young and matures oil, respectively.
Reports of the chemical composition of the essential oil from Artemisia species also included A. argyi, which is a widely used traditional Chinese medicine. Li et al. [32] reported for the first time the separation and identification of volatile constituents in this plant by GC/MS. The main components are borneol ( Figure 1) and bornyl acetate. The chemical constituents of the essential oil from A. rubripes were also investigated [33]. The volatile oil was extracted from leaves by steam distillation and the components of the oil were separated and identified by GC/MS. The main components of volatile oil were camphor (26.9%), 1,8-cineole (15.6%), β-caryophyllene (13.3%) and germacrene D (5.4%). Yang et al. [34] investigated the chemical composition of the essential oil of another Chinese Artemisia species, Artemisia ordosica Krasch. The main composition and the composition fraction of volatile oil of this plant were analyzed by GC/MS, and 37 components, representing 90% of the total oil were identified, which mainly included 17 terpenoids, 14 alcohols, two esters, two ketones and two other components. The main constituents were β-bisabolol (27%), α-cadinol (26.4%), epiglobulol (25.6%) and cis-lanceol (25%). The volatiles chemical constituents of Artemisia capillaris Thunb., another important Chinese medicine, were also determined by GC/MS and sub-window factor analysis [35]. A total of 75 components were separated and 43 of them were qualitatively and quantitatively determined, which represented about 89% of the total content. The main constituents were 9,12,15-octadecatrienal (34.5%), phytol (33.6%) and cyclopentaneundecanoic acid (33.1%).
Members of the Artemisia genus have also been reported in Iran. The aerial parts of A. spicigera collected from Azerbaijan province, were subjected to steam distillation in Clevenger apparatus, and a light yellow residue was obtained [36]. The essential oil was submitted to careful analysis by GC and GC/MS, and the results showed the presence of approximately 17 components, with camphor (24.6%), 1,8-cineole (23.3%) (Figure 4), β-thujone (20.7%) ( Figure 2) and α-thujone as the major components in this plant. The essential oil has a very strong odour and an acrid taste, and is described as neurotoxic due to its high thujone content. The composition of the volatile oil in A. spicigera varies widely according to the geographical location, climate, day length, soil type and cultivar. Rezaeinodehl and Khangholi [37] investigated the chemical composition of the essential oil of A. absinthium growing wild in Iran. The aerial parts were harvested at full bloom from an area between the villages of Deylamon and Asiabar, in Guilau province. Results showed that 28 components were identified, representing 93.3% of the oil, most of which were monoterpenes. The main components were β-pinene (23.8%) and β-thujone (18.6%) (Figure 2). The fresh aerial parts of A. dracunculus cultivated in Karadj (west of Tehran) were subjected to hydrodistillation using a Clevenger apparatus, and the oil analyzed by GC/MS [38]. The results revealed the presence of trans-anethole (21.1%), trans-αocimene (20.6%), limonene (12.4%) (Figure 3), α-pinene (5.1%), cis-β-ocimene (4.8%), methyl eugenol (2.2%), β-pinene (0.8%), α-terpinolene (0.5%), bornyl acetate (0.5%) and bicyclogermacrene (0.5%) as the main components.
A novel method was developed for extraction and analysis of the volatile compounds of A. haussknechtii using simultaneous hydrodistillation and static head space liquid microextraction followed by GC/MS [39]. The main constituents of this essential oil included camphor (41%),  The intraspecific chemical variability of essential oils (50 samples) isolated from the aerial parts of A. herba-alba growing wild in the arid zone of southeastern Tunisia was investigated [41]. Analysis by GC and GC/MS allowed the identification of 54 essential oil components. The main compounds were β-thujone ( Figure 2) and α-thujone, followed by 1,8-cineole (Figure 4), camphor, chrysanthenone, trans-sabinyl acetate, trans-pinocarveol and borneol (Figure 1). Wild plants of A. herba-alba randomly harvested in the area of Kirchaou and transplanted by farmers for cultivation in arid zones of southern Tunisia produced an essential oil belonging to the α-thujone/β-thujone chemotype, and containing also 1,8-cineole (Figure 4), camphor and trans-sabinyl acetate in appreciable amounts [42]. From the essential oil from this species, A. herba-alba growing in Pakistan, Shah et al. [43] identified 25 compounds, composing 93.7% of the oil. Among these, chrysanthenyl propionate and elixene were identified for the first time from any Artemisia species.

Anti-infective Effects of Essential Oils from the Artemisia Genus
Essential oils have been largely employed for their properties which are already observed in Nature. Known for their antiseptic (bactericidal, virucidal and fungicidal) and medicinal properties and their fragrance, they are used in embalment, preservation of foods, and as microbicidal, analgesic, sedative, anti-inflammatory, spasmolytic and locally anaesthetic remedies [48]. At present, approximately 3,000 essential oils are known, 300 of which are commercially important, especially for the pharmaceutical, agricultural, food, sanitary, cosmetics and perfume industries. Essential oils or some of their components are used in perfumes and make-up products, in sanitary products, in dentistry, in agriculture, as food preservatives and additives and as natural remedies. These characteristics have remained largely unchanged to the present day, except that more is known about some of their mechanisms of action, particularly at the antimicrobial level. Lipophilic constituents of essential oils, either inhaled or topically applied, have been suggested to successfully inhibit microbial growth by means of reacting with the lipid parts of the cell membranes, rendering more permeable microbial cell membranes and mitochondria membrane, which results in death of bacterial cell after massive ion leakage and interrupting enzyme function in specific metabolic pathways. Essential oils are also able to inhibit the synthesis of DNA, RNA, proteins and polysaccharides in bacterial cells.
Lopez-Lutz et al. [54] used GC/MS to investigate the chemical composition and antimicrobial activity of essential oil isolated from aerial parts of seven wild sages from western Canada Nibret and Wink [55] investigated the in vitro antitrypanosomal activity of four Ethiopian Artemisia species (A. absinthium, A. abyssinica, A. afra and A. annua). The essential oil from their leaves and aerial parts was tested in vitro against bloodstream forms of Trypanosoma brucei brucei. These studies validated the claims of the traditional medicinal uses of the four Artemisia species for the treatment of protozoal infections, in this particular case against trypanosomes. Camphor was detected in the four species and was found to be the principal compound (38.7%). The essential oil of two of these Ethiopian Artemisia species, A. absinthium and A. abyssinica, also showed activity against promastigote and axenic amastigote forms of two Leishmania strains, Leishmania aethiopica and Leishmania donovani [56,57]. These results demonstrated the potential use of both oils as a source of novel agents for the treatment of leishmaniasis. The oil of the species A. absinthium was also tested against eleven pathogenic bacterial strains [58]. Lysteria monocytogenes, Bacillus cereus and Staphylococcus aureus were the most sensitive bacteria with a minimum inhibitory concentration (MIC) of 0.14, 0.8 and 0.62 μL/mL, respectively. The essential oil of A. annua also presented toxic repellent and development inhibitory activities against two economically important stored product insects: Tribolium castaneum and Callosobruchus maculates [59]. Adult beetles were repelled significantly by the oil of A. annua at 1% concentration and in the filter paper arena test. O Three species of the genus, A. scoparia, Artemisia sieberi Bess and Artemisia aucheri Boiss, widely distributed in the desert area of Iran, were screened for antifungal and anti-infective activities on some soil-borne phytopathogens and insects [60][61][62]. The essential oils were obtained by hydro-distillation of air-dried samples and their chemical composition identified by GC/MS. Oxygenated monoterpenes were the major components of the oils of the three species: α-thujone (81.7%), β-thujone (14.5%) ( Figure 2) and 1,8-cineole (1.9%) (Figure 4) were the major compounds in the essential oil of A. scoparia; the essential oil of A. aucheri was rich in linalool (44.1%), geranyl acetate (10.7%), (E)-citral (9.7%) and (Z)-citral (7.7%); and the essential oil of A. sieberi was rich in β-thujone (19.8%) (Figure 2), α-thujone (10.5%), camphor (19.5%), verbenol (9.7%), p-mentha-1,5-dien-8-ol (6.4%) and davanone (5.8%) ( Figure 6). Results of bioassay showed that the oils of A. aucheri and A. sieberi have stronger antifungal activity against some soil-borne pathogenic fungi such as Rhizoctonia solani, Tiarospella phaseolina, Fusarium moniliforme and Fusarium solani, while the oils of A. scoparia and A. sieberi against three coleopteran stored-product insects (Callosobruchus maculates, Sitophilus oryzae and Tribolium castaneum). The insecticidal constituents of these essential oils are monoterpenoids. Due to their high volatility, they have fumigant activity that might be of importance for controlling stored-product insects.
Cha et al. [63] used GC/MS to analyze the chemical composition of the essential oil obtained from one of these species from South Korea, A. scoparia. The essential oil was rich in camphor (11%), 1,8-cineole (21.5%) ( Figure 4) and β-caryophyllene (6.8%). The essential oil and its major compounds were tested for their antimicrobial activity against fifteen different genera of oral bacteria, exhibiting various degrees of growth inhibition. The essential oil from this species A. scoparia from India also presents phytotoxic effects against five weed species, suggesting its possible use as a bioherbicide [64]. GC/MS analysis revealed the essential oil (yield 0.84%) is a complex mixture containing 19 monoterpenes, seven sesquiterpenes and 15 other compounds (aliphatic alcohols, ketones, aromatic hydrocarbons and esters) [65]. The main monoterpenes were β-myrcene (30.2%), p-cymene (12.8%) and limonene (12.4%) (Figure 3). The three monoterpenes exhibited phytotoxicity and reduced germination, seedling growth and chlorophyll content of Arena sativa and Triticum aestivum in a dose-dependent manner. The essential oil of A. scoparia and its active constituents inhibits plant growth by generating reactive oxygen species (lipid peroxidation, membrane integrity and amounts of conjugated dienes and hydrogen peroxide), and causing oxidative damage [66]. Inhibition of germination and root growth by oil from foliage of A. scoparia suggests that under natural conditions, these volatile terpenes may emanate from the plant, enter the soil, and may be involved in suppression of associated vegetation, thus resulting in the formation of its monospecific strands. The essential oil from this species, A. scoparia, together with Artemisia mongolica (Fisch. ex Besser) Nakai, was also found to possess insecticidal activity against the maize weevil Sitophilus zeamais [67]. The main constituents of A. mongolica essential oil were α-pinene (12.6%), germacrene D (8.3%) and γ-terpinene (8.1%).
Militello et al. [68] investigated the chemical composition of essential oil from A. arborescens growing wild in Sicily, Italy. Essential oil extracted by steam distillation was also examined for its capability to inhibit some food-borne pathogen bacteria. A total of 43 compounds, accounting for 93.7% of the total oil, were identified by GC and GC/MS. Oxygenated monoterpenes (57.3%) constituted the main fraction, with β-thujone (45%) (Figure 2) as the main compounds, followed by the sesquiterpene hydrocarbon chamazulene (22.7%). Undiluted essential oil showed a large inhibition spectrum against strains of Lysteria monocytogenes, whilst it was ineffective against enterobacteria and Salmonella; thus, it may represent a natural preservative, alternative to the common chemical additives. This essential oil also present antiherpes virus activity, principally due to direct virucidal effect [69]. The mode of action of the essential oil as an antiherpes virus agent appears particularly interesting due to its ability to inactivate the virus and to inhibit cell-to-cell virus diffusion.
Reports on the chemical composition and anti-infective activity of other Artemisia species from China were also found in the literature. The essential oil of A. argyi obtained by supercritical carbon dioxide extraction and hydro-distillation was analyzed by GC/MS to characterize its components, and also tested for antifungal activity [72]. A total of 61 compounds were identified, of which the most abundant were 1,8-cineole (4.4%) (Figure 4), borneol (3.5%) (Figure 1), terpinol (10.1%), spathulenol (10%) (Figure 7), caryophyllene oxide (6.5%), juniper camphor (8.7%), chamazulene (2%) and camphor (3.4%). The essential oil extracted by these two methods exhibited antifungal activity against Botrytis cinerea and Alternaria alternata, two common storage pathogens of fruits and vegetables. The inhibition of these fungi was 93.3% and 84.7% for oil extracted by hydrodistillation when exposed to a concentration of 1 mg/mL, while values of 70.8% and 60.5% were observed from oil extracted by supercritical carbon dioxide. The essential oil from A. princeps, a Chinese medicinal plant widespread in China, exhibited repellent activities against the beetles Sitophillus orizae and Bruchus rugimanus at concentrations ranging from 250 to 1000 μg/g [73]. The essential oil from this species, A. princeps, and its main constituents, 1,8-cineole and α-terpineol, also ameliorated bacterial vaginosis and vulvovaginal candidiasis in mice by inhibiting bacterial growth and nuclear factor-κB activation [74]. From the two compounds, α-terpineol most potently inhibited the growths of Gardnerella vaginalis and Candida albicans with MIC values of 0.06 and 0.125% (v/v), respectively. From another Chinese Artemisia species, A. annua, 1,8-cineole (Figure 4) has been identified as an anti-infective constituent [75]. The compound inhibited the growth of Trypanosoma brucei brucei, the causative agent of Nagana epidemic, with an inhibitory concentration 50 (IC 50 ) value of 64.6 μg/mL.
The antifungal, antibacterial and insect repellent activities of the essential oil from Artemisia mendozana DC., a medicinal plant from the central Andes in Argentina, were also investigated [81]. However, the oil showed only moderate activity.
The leaf oil of A. douglasiana has been shown to be an efficacious complementary herbal treatment for chronic bladder infection. The oil has been analyzed by GC/MS and the major components found to be camphor (29%), artemisia ketone (26%) (Figure 5), artemisia alcohol (13%), α-thujone (10%), 1,8-cineole (8%) ( Figure 4) and hexanal (5%) [82]. The leaf oil and the major components have been tested for antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. The essential oil shows limited antimicrobial activity in vitro, so it is unclear if the oil exerts a direct antimicrobial effect in vivo or plays some role in stimulating the host defences. From this species, vulgarone B ( Figure 10) has been identified as the active antifungal constituent [83]. One of the possible modes of this antifungal compound may be due to their role as Michael-type acceptor for biological nucleophiles. For the studies of structure-activity relationships, it was postulated that the α,β-unsaturated carbonyl group is essential for the antifungal activity of vulgarone B. This compound, vulgarone B, has also been reported in the essential oil from A. iwayomogi as an antibacterial constituent [84]. The antibacterial activities of vulgarone B have been demonstrated against some antibiotic-susceptible and -resistant human pathogens, and the antibiotic mechanism involved might be related to DNA cleavage.  The antifungal activity of another Artemisia species, A. herba-alba, was found to be associated with two major volatile compounds isolated from the fresh leaves of the plant [85]. Carvone and piperitone were isolated and identified by GC/MS. Antifungal activity was measured against Penicillium citrinum and Mucora rouxii. The antifungal activity of the purified compounds (carvone and piperitone) was estimated to be effective against Penicillium citrinum (IC 50 of 5 and 2 μg/mL), and against Mucora rouxii (IC 50 of 7 and 1.5 μg/mL), respectively. The essential oil from this species, A. herba-alba, growing wild in southwest of Tunisia, also presents antimicrobial activity against six bacterial strains and three fungal strains, with IC 50 values of 8-51 μg/mL [86].

Conclusions
This review summarizes and characterizes the importance of essential oils found from a wide range of Artemisia species. A number of compounds of these oils (and the oils themselves) have medicinal and (ethno-) pharmacological properties. The potential for developement of leads from Artemisia continues to grow, particularly in the area of infectious conditions. The information summarize here is intended to serve as a reference tool to people in all fields of ethnopharmacology and natural products chemistry.