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Open AccessFeature PaperArticle

Features and Advantages of Supercritical CO2 Extraction of Sea Cucumber Cucumaria frondosa japonica Semper, 1868

1
School of Biomedicine, Department of Pharmacy and Pharmacology, Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, Russia
2
N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Sankt-Peterburg, Russia
3
NhaTrang Institute of Technology Research and Application, VAST, Nhatrang, Nha Trang City 57000, Vietnam
4
Heze Branch, Qilu University of Technology (Shandong Academy of Sciences), Biological Engineering Technology Innovation Center of Shandong Province China, Heze 274008, China
5
Key Laboratory for Biosensor of Shandong Province, Biology Institute, Jinan 250013, China
6
Department of Biotechnology, Chonnam National University, Yeosu 59626, Korea
*
Author to whom correspondence should be addressed.
Molecules 2020, 25(18), 4088; https://doi.org/10.3390/molecules25184088
Received: 14 July 2020 / Revised: 26 August 2020 / Accepted: 2 September 2020 / Published: 7 September 2020
Extraction process of Cucumaria frondosa japonica Semper, 1868, which are subspecies of Cucumaria frondosa (Gunnerus, 1767), were studied. It was shown that supercritical carbon dioxide extraction of holothuria was more effective than conventional solvent extraction. Step-by-step extraction with carbon dioxide followed by supercritical extraction with the addition of a co-solvent of ethanol can almost double the yields of extracts of triterpene glycosides, styrenes and carotenoids. Moreover, the fraction of triterpene glycosides practically does not contain colored impurities, in contrast to traditional ethanol extraction. The obtained extracts by HPLC in combination with tandem mass spectrometry (HPLC-MS/MS) identified 15 triterpene glycosides, 18 styrene compounds and 14 carotenoids. Supercritical extraction made it possible to obtain extracts with yields superior to conventional hexane and alcohol extracts. Moreover, such an approach with the use of supercritical fluid extraction (SFE) and subsequent profiling of metabolites can help with the study of holothuria species that are not as well studied. View Full-Text
Keywords: sea cucumber; supercritical extraction; Cucumaria frondosa japonica; triterpene glycosides; saponins; carotenoids; tandem mass spectrometry sea cucumber; supercritical extraction; Cucumaria frondosa japonica; triterpene glycosides; saponins; carotenoids; tandem mass spectrometry
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MDPI and ACS Style

Zakharenko, A.; Romanchenko, D.; Thinh, P.D.; Pikula, K.; Hang, C.T.T.; Yuan, W.; Xia, X.; Chaika, V.; Chernyshev, V.; Zakharenko, S.; Razgonova, M.; Chung, G.; Golokhvast, K. Features and Advantages of Supercritical CO2 Extraction of Sea Cucumber Cucumaria frondosa japonica Semper, 1868. Molecules 2020, 25, 4088. https://doi.org/10.3390/molecules25184088

AMA Style

Zakharenko A, Romanchenko D, Thinh PD, Pikula K, Hang CTT, Yuan W, Xia X, Chaika V, Chernyshev V, Zakharenko S, Razgonova M, Chung G, Golokhvast K. Features and Advantages of Supercritical CO2 Extraction of Sea Cucumber Cucumaria frondosa japonica Semper, 1868. Molecules. 2020; 25(18):4088. https://doi.org/10.3390/molecules25184088

Chicago/Turabian Style

Zakharenko, Alexander; Romanchenko, Denis; Thinh, Pham D.; Pikula, Konstantin; Hang, Cao T.T.; Yuan, Wenpeng; Xia, Xuekui; Chaika, Vladimir; Chernyshev, Valery; Zakharenko, Svetlana; Razgonova, Mayya; Chung, Gyuhwa; Golokhvast, Kirill. 2020. "Features and Advantages of Supercritical CO2 Extraction of Sea Cucumber Cucumaria frondosa japonica Semper, 1868" Molecules 25, no. 18: 4088. https://doi.org/10.3390/molecules25184088

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