Potent and Non-Cytotoxic Antibacterial Compounds Against Methicillin-Resistant Staphylococcus aureus Isolated from Psiloxylon mauritianum, A Medicinal Plant from Reunion Island
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Plant Material
3.3. Extraction and Isolation
3.4. Determination of Minimal Inhibitory Concentration
3.5. Cytotoxicity Evaluation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Position | Aspidin VB | ||||
---|---|---|---|---|---|
δC1 | δH(J in Hz) 2 | COSY | HMBC | ROESY | |
1 | 111.9 | ||||
2 | 188.4 | ||||
3 | 108.9 | ||||
4 | 199.9 | ||||
5 | 45.1 | ||||
6 | 172.7 | ||||
7 | 17.7 | 3.57, s | C1, C2, C6, C1′, C2′, C6′ | ||
8 | 207.5 | ||||
9 | 41.6 | 3.19, dd (7.2, 7.2) | H8 | C3, C8, C10, C11 | H11 |
10 | 25.3 | 1.67, m | H9, H11 | C8, C9, C11, C12 | |
11 | 32.5 | 1.39, m | H10, H12 | C12 | H9 |
12 | 23.2 | 1.38, m | H11, H13 | C11 | |
13 | 14.3 | 0.93, t (7.1) | H12 | C11, C12 | |
1′ | 110.2 | ||||
2′ | 160.6 | ||||
3′ | 108.5 | ||||
4′ | 161.5 | ||||
5′ | 113.2 | ||||
6′ | 163.6 | ||||
7′ | 62.1 | 3.80, s | C4′ | H9′ | |
8′ | 208.0 | ||||
9′ | 44.8 | 3.15, dd (7.2, 7.2) | H10′ | C8′, C10′, C11′ | H7′ |
10′ | 18.8 | 1.72, sex (7.4) | H9′, H11′ | C8′, C10′, C11′ | |
11′ | 14.2 | 0.98, t (7.4) | H10′ | C9′, C10′ | H9′ |
12′ | 9.5 | 2.10, s | C4′, C5′, C6′ | H7′ | |
6-OH | 10.05, s | C1, C5, C6 | H7, 2′-OH, 6′-OH | ||
2′-OH | 15.86, s | C1′, C3′, C8′ | 6-OH | ||
6′-OH | 11.41, s | C1′, C5′, C6′ | H7, 6-OH |
Compounds | MIC (µg/mL) | MRC5 Cell Viability (%) | ||||
---|---|---|---|---|---|---|
C. albicans ATCC 10213 | T. rubrum SNB-TR1 | S. aureus ATCC 29213 | MRSA ATCC 33591 | 10−5 M | 10−6 M | |
1 | >256 | >256 | 2 | 1 | 86 ± 3 | 104 ± 1 |
2 | >256 | 256 | 0.25 | 0.25 | 99 ± 2 | 105 ± 2 |
Crude extract | 8 | 256 | 8 | nd | 96 ± 2 | 100 ± 3 |
Fluconazole 1 | 1 | 4 | nd | nd | nd | nd |
Itraconazole 1 | <0.5 | <0.5 | nd | nd | nd | nd |
Oxacillin 1 | nd 2 | nd | 0.25 | nd | nd | nd |
Vancomycin 1 | nd | nd | nd | 4 | nd | nd |
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Sorres, J.; André, A.; Elslande, E.V.; Stien, D.; Eparvier, V. Potent and Non-Cytotoxic Antibacterial Compounds Against Methicillin-Resistant Staphylococcus aureus Isolated from Psiloxylon mauritianum, A Medicinal Plant from Reunion Island. Molecules 2020, 25, 3565. https://doi.org/10.3390/molecules25163565
Sorres J, André A, Elslande EV, Stien D, Eparvier V. Potent and Non-Cytotoxic Antibacterial Compounds Against Methicillin-Resistant Staphylococcus aureus Isolated from Psiloxylon mauritianum, A Medicinal Plant from Reunion Island. Molecules. 2020; 25(16):3565. https://doi.org/10.3390/molecules25163565
Chicago/Turabian StyleSorres, Jonathan, Amandine André, Elsa Van Elslande, Didier Stien, and Véronique Eparvier. 2020. "Potent and Non-Cytotoxic Antibacterial Compounds Against Methicillin-Resistant Staphylococcus aureus Isolated from Psiloxylon mauritianum, A Medicinal Plant from Reunion Island" Molecules 25, no. 16: 3565. https://doi.org/10.3390/molecules25163565