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Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts
valued for its horticultural as well as therapeutic benefits. The present study set out to investigate
the inhibitory activity of A. pyramidalis tubers against key biological targets for the management of
type 2 diabetes, Alzheimer disease, and skin hyperpigmentation. In addition, the antioxidant
potential of the extracts was also assessed using multiple methods. The detailed phytochemical
profiles of the extracts were determined using high‐performance liquid chromatography. Based on
qualitative phytochemical fingerprint, a network pharmacology analysis was conducted as well.
Parishin was identified from the water extract only, whereas gastrodin and caffeic acid derivatives
were present in the methanol extract. The methanol extract exhibited high inhibitory activity
against tyrosinase (69.69 mg kojic acid equivalent/g extract), α‐amylase (15.76 mg acarbose
equivalent/g extract), and α‐glucosidase (20.07 mg acarbose equivalent/g extract). Similarly, the
methanol extract showed highest antioxidant potential (22.12, 44.23, 45.56, and 29.38 mg Trolox
equivalent/g extract, for 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), 2,2ʹ‐azino‐bis(3‐
ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), CUPric Reducing Antioxidant Capacity (CUPRAC),
and Ferric Reducing Antioxidant Power (FRAP) assays, respectively). Finally, the results of
network pharmacology analysis, besides corroborating traditional uses of plant extracts in the
management of cold and flu, confirmed a direct involvement of identified phytochemicals in the
observed enzyme inhibitory effects, especially against tyrosinase, α‐amylase, and α‐glucosidase.
Furthermore, based on the results of both colorimetric assays and network pharmacology analysis related to the activity of A. pyramidalis extracts and identified phytocompounds on enzymes
involved in type 2 diabetes, a docking study was conducted in order to investigate the putative
interactions of oxo‐dihydroxy octadecenoic acid trihydroxy octadecenoic acid against aldose
reductase, peroxisome proliferator‐activated receptor (PPAR)‐α, dipeptidyl peptidase (DPP)‐IV,
and α‐glucosidase. Docking analysis suggested the inhibitory activity of these compounds against
the aforementioned enzymes, with a better inhibitory profile shown by oxo‐dihydroxy
octadecenoic acid. Overall, the present findings supported the rationale for the use of A.
pyramidalis as source of bioactive metabolites and highlight, today more than ever, for the strong
necessity of linkage strategy between wild resource valorization and conservation policy.
Mahomoodally, M.F.; Picot-Allain, M.C.N.; Zengin, G.; Llorent-Martínez, E.J.; Abdullah, H.H.; Ak, G.; Senkardes, I.; Chiavaroli, A.; Menghini, L.; Recinella, L.; Brunetti, L.; Leone, S.; Orlando, G.; Ferrante, C. Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts. Molecules 2020, 25, 2422.
Mahomoodally MF, Picot-Allain MCN, Zengin G, Llorent-Martínez EJ, Abdullah HH, Ak G, Senkardes I, Chiavaroli A, Menghini L, Recinella L, Brunetti L, Leone S, Orlando G, Ferrante C. Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts. Molecules. 2020; 25(10):2422.Chicago/Turabian Style
Mahomoodally, Mohamad F.; Picot-Allain, Marie C.N.; Zengin, Gokhan; Llorent-Martínez, Eulogio J.; Abdullah, Hassan H.; Ak, Gunes; Senkardes, Ismail; Chiavaroli, Annalisa; Menghini, Luigi; Recinella, Lucia; Brunetti, Luigi; Leone, Sheila; Orlando, Giustino; Ferrante, Claudio. 2020. "Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts." Molecules 25, no. 10: 2422.