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A Rapid UPLC-MS Method for Quantification of Gomisin D in Rat Plasma and Its Application to a Pharmacokinetic and Bioavailability Study

School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China
Author to whom correspondence should be addressed.
Academic Editors: Isabel C.F.R. Ferreira and Nancy D. Turner
Molecules 2019, 24(7), 1403;
Received: 19 March 2019 / Revised: 2 April 2019 / Accepted: 7 April 2019 / Published: 10 April 2019
(This article belongs to the Collection Bioactive Compounds)
PDF [780 KB, uploaded 10 April 2019]


Gomisin D, a lignan compound isolated from Fructus Schisandra, is a potential antidiabetic and anti-Alzheimer’s agent. Recently, gomisin D was used as a quality marker of some traditional Chinese medicine (TCM) formulas. In this study, a rapid ultra-performance liquid chromatography/tandem mass spectrometry method (UPLC-MS/MS) was developed and validated to quantify gomisin D in rat plasma for a pharmacokinetic and bioavailability study. Acetonitrile was used to precipitate plasma proteins. Separations were performed on a BEH C18 column with a gradient mobile phase comprising of acetonitrile and water (0.1% formic acid). An electrospray ionization source was applied and operated in the positive ion mode. The multiple reaction monitoring mode (MRM) was utilized to quantify gomisin D and nomilin (internal standard, IS) using the transitions of m/z 531.2 → 383.1 and m/z 515.3 → 161.0, respectively. The calibration curve was linear over the working range from 1 to 4000 ng/mL (R2 = 0.993). The intra- and interday precision ranged from 1.9% to 12.9%. The extraction recovery of gomisin D was in the range of 79.2–86.3%. The validated UPLC-MS/MS method was then used to obtain the pharmacokinetic characteristics of gomisin D after intravenous (5 mg/kg) and intragastric (50 mg/kg) administration to rats. The bioavailability of gomisin D was 107.6%, indicating that this compound may become a promising intragastrical medication. Our results provided useful information for further preclinical studies on gomisin D. View Full-Text
Keywords: UPLC-MS/MS; gomisin D; pharmacokinetic; bioavailability UPLC-MS/MS; gomisin D; pharmacokinetic; bioavailability

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Zheng, X.; Feng, F.; Jiang, X.; Qiu, J.; Cai, X.; Xiang, Z. A Rapid UPLC-MS Method for Quantification of Gomisin D in Rat Plasma and Its Application to a Pharmacokinetic and Bioavailability Study. Molecules 2019, 24, 1403.

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