Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways
Chang Liu 1,†
, Hong Liu 1,†, Yanzhang Wen 2, Huiqi Huang 2, Ji Hao 2, Yibing Lv 2, Rui Qin 1,* and Xinzhou Yang 2,*
, Hong Liu 1,†, Yanzhang Wen 2, Huiqi Huang 2, Ji Hao 2, Yibing Lv 2, Rui Qin 1,* and Xinzhou Yang 2,*
1
College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China
2
School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2019, 24(6), 1074; https://doi.org/10.3390/molecules24061074
Received: 3 March 2019 / Revised: 14 March 2019 / Accepted: 15 March 2019 / Published: 19 March 2019
(This article belongs to the Collection Natural Products: Anticancer Potential and Beyond)
Abstract
Aspernolide A, a butyrolactone secondary metabolite, was purified from the endophytic fungus Cladosporium cladosporioides derived from roots of Camptotheca acuminata Decne. In this study, the antitumor activity and mechanisms of aspernolide A on human laryngeal cancer Hep-2 and TU212 cells were studied by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, morphological observation and Western blotting. The results showed that aspernolide A significantly inhibited the proliferation of Hep-2 and TU212 cells in dose- and time-dependent manners. Morphological changes of apoptotic cells could be observed under an inverted microscope, such as irregular margins, decreased adherence ability and chromatin condensation. The expressions of Bax, Caspase-9, Caspase-3 and PARP (poly ADP-ribose polymerase) increased with the increase of dosage while Bcl-2 decreased, suggesting that the apoptotic mechanism might be related to the mitochondrial apoptotic pathway. Moreover, the expression of the phosphorylation of STAT3 decreased with the increase of dosage, suggesting that the apoptotic mechanism might be related to the STAT3 signaling pathway. All these conclusions indicated that aspernolide A has the potential anti-laryngocarcinoma effects. View Full-TextKeywords:
aspernolide A; camptotheca acuminata decne; cladosporium cladosporioides; anticancer; laryngeal cancer; apoptosis; mitochondrial pathway; STAT3
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Liu, C.; Liu, H.; Wen, Y.; Huang, H.; Hao, J.; Lv, Y.; Qin, R.; Yang, X. Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways. Molecules 2019, 24, 1074.
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