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Molecules 2019, 24(6), 1019; https://doi.org/10.3390/molecules24061019

Development of a Bioactive Sauce Based on Oriental Mustard Flour with Antifungal Properties for Pita Bread Shelf Life Improvement

Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain
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Authors have contributed equally to the study and should be considered co-first authors.
Academic Editor: Chiara Dall’Asta
Received: 11 February 2019 / Revised: 27 February 2019 / Accepted: 12 March 2019 / Published: 14 March 2019
(This article belongs to the Special Issue Mycotoxins)
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Abstract

Ochratoxin A (OTA) is a mycotoxin produced in the secondary metabolism of fungus belonging to the genus Aspergillus and Penicillium. In this study, the employment of oriental mustard flour (OMF) as an ingredient in a packaged sauce was evaluated for the generation in situ of the antimicrobial compound allyl isothiocyanate (AITC) in order to preserve pita bread contaminated with Penicillium verrucosum VTT D-01847, an OTA producer, in an active packaging system. Four different concentrations (8, 16, 33 and 50 mg/g) were tested. Mycelium formation, mycotoxin production, AITC absorbed by the food matrix, and volatilization kinetics were studied for each concentration. The results obtained were compared with bread treated with the commercial additive calcium propionate (E-282). The results showed a shelf life increase of two and three days with the employment of 33 and 50 mg/g of OMF, with a significant reduction of the fungal population (3.1 and 5.7 logs, respectively) in comparison with the control experiment. The use of 16 and 33 mg/g of OMF in the sauce formulation decreased the concentration of OTA in the bread samples while no OTA production was detected employing 50 mg/g of OMF. View Full-Text
Keywords: active packaging; antifungal properties; AITC; bread; shelf life; OTA active packaging; antifungal properties; AITC; bread; shelf life; OTA
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Torrijos, R.; Nazareth, T.M.; Pérez, J.; Mañes, J.; Meca, G. Development of a Bioactive Sauce Based on Oriental Mustard Flour with Antifungal Properties for Pita Bread Shelf Life Improvement. Molecules 2019, 24, 1019.

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