Next Article in Journal
In Vitro Infant Faecal Fermentation of Low Viscosity Barley β-Glucan and Its Acid Hydrolyzed Derivatives: Evaluation of Their Potential as Novel Prebiotics
Next Article in Special Issue
Formation of Ethyl Carbamate during the Production Process of Cantonese Soy Sauce
Previous Article in Journal
Prediction Models to Control Aging Time in Red Wine
Article Menu
Issue 5 (March-1) cover image

Export Article

Open AccessArticle

Chromo-Fluorogenic Detection of Soman and Its Simulant by Thiourea-Based Rhodamine Probe

Research Institute of Chemical Defense, Beijing 102205, China
State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
Laboratory of Organic NanoPhotonics and CAS Key Laboratory of Bio-Inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Authors to whom correspondence should be addressed.
These authors have contributed equally to this work.
Academic Editor: Simone Morais
Molecules 2019, 24(5), 827;
Received: 3 February 2019 / Revised: 18 February 2019 / Accepted: 18 February 2019 / Published: 26 February 2019
(This article belongs to the Special Issue Advanced Analysis of Contaminants of Emerging Concern )
PDF [7846 KB, uploaded 26 February 2019]


Here, we introduced a novel thiourea-based rhodamine compound as a chromo-fluorogenic indicator of nerve agent Soman and its simulant diethyl chlorophosphate (DCP). The synthesized probe N-(rhodamine B)-lactam-2-(4-cyanophenyl) thiourea (RB-CT), which has a rhodamine core linked by a cyanophenyl thiosemicarbazide group, enabled a rapidly and highly sensitive response to DCP with clear fluorescence and color changes. The detection limit was as low as 2 × 10−6 M. The sensing mechanism showed that opening of the spirolactam ring following the phosphorylation of thiosemicarbazides group formed a seven-membered heterocycle adduct, according to MS analysis and TD-DFT calculations. RB-CT exhibited high detecting selectivity for DCP, among other organophosphorus compounds. Moreover, two test kits were employed and successfully used to detect real nerve agent Soman in liquid and gas phase. View Full-Text
Keywords: Nerve agents; Soman; Rhodamine B; Thiosemicarbazide; Chromo-fluorogenic probe; Detection Nerve agents; Soman; Rhodamine B; Thiosemicarbazide; Chromo-fluorogenic probe; Detection

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Li, S.; Zheng, Y.; Chen, W.; Zheng, M.; Zheng, H.; Zhang, Z.; Cui, Y.; Zhong, J.; Zhao, C. Chromo-Fluorogenic Detection of Soman and Its Simulant by Thiourea-Based Rhodamine Probe. Molecules 2019, 24, 827.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top