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Molecules 2019, 24(3), 618; https://doi.org/10.3390/molecules24030618

Lysosome-Targeted Single Fluorescence Probe for Two-Channel Imaging Intracellular SO2 and Biothiols

School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
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Received: 16 January 2019 / Revised: 3 February 2019 / Accepted: 4 February 2019 / Published: 11 February 2019
(This article belongs to the Section Analytical Chemistry)
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Abstract

As the members of reactive sulfur species, SO2 and biothiols play a significant role in physiological and pathological processes and directly influence numerous diseases. Furthermore, SO2 and biothiols can provide a reductive environment for lysosomes to carry out their optimal functionality. To this end, the development of single fluorescent probes for imaging SO2 and biothiols from different emission channels is highly desirable for understanding their physiological nature. Here, a lysosome-targeted fluorescent probe (BPO-DNSP) with a dual reaction site for SO2 and biothiols was presented. BPO-DNSP can sensitively and selectively respond to SO2 in the green channel with a large Stokes shift over 105 nm, and to biothiols in the near-infrared emission channel with a large Stokes shift over 109 nm. The emission shift for the two channels was as high as 170 nm. Colocalization experiments verified that BPO-DNSP can selectively enrich lysosomes. Notably, BPO-DNSP can not only be used to image intracellular SO2 and biothiols from two different channels, but also to monitor the conversion of biothiols to SO2 without adding exogenous enzymes in living HeLa cells. View Full-Text
Keywords: single fluorescent probe; lysosome-targeted; SO2; biothiols; fluorescence imaging single fluorescent probe; lysosome-targeted; SO2; biothiols; fluorescence imaging
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Wang, Y.; Liu, L.; Zhou, X.-L.; Wu, M.-Y. Lysosome-Targeted Single Fluorescence Probe for Two-Channel Imaging Intracellular SO2 and Biothiols. Molecules 2019, 24, 618.

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