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Molecules 2019, 24(3), 619; https://doi.org/10.3390/molecules24030619

HPLC Profile of Longan (cv. Shixia) Pericarp-Sourced Phenolics and Their Antioxidant and Cytotoxic Effects

1
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 10048, China
2
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
3
School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China
*
Authors to whom correspondence should be addressed.
Received: 4 January 2019 / Revised: 31 January 2019 / Accepted: 5 February 2019 / Published: 11 February 2019
(This article belongs to the Special Issue The Antioxidant Capacities of Natural Products 2019)
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Abstract

Longan (Dimocarpus longan Lour.) pericarp, the main by-product of aril and pulp processing, is abundant in phenolic compounds and worthy of further utilization. The present work firstly reported HPLC analysis and in vitro antioxidant evaluation of longan (cv. Shixia) pericarp-derived phenolics (LPPs), the purified longan pericarp extract (LPE), as well as their cytotoxic effect on lung cancer cell line, A549. The results indicated that the purified LPE had significant amounts of phenolics, with content of 57.8 ± 0.6 mg of gallic acid equivalents per gram of dry longan pericarp (mg GAE·g−1 DLP), which consisted of six phenolic compounds (A–F), including protocatechuic acid (A), isoscopoletin (B), quercetin (C), ellagic acid (D), corilagin (E), and proanthocyanidins C1 (F). Antioxidant assays showed that LPPs (10 μM) and LPE (1.0 mg·mL−1) had certain antioxidant activities, in which corilagin (E) possessed the best DPPH radical scavenging rate 71.8 ± 0.5% and •OH inhibition rate 75.9 ± 0.3%, and protocatechuic acid (A) exhibited the strongest Fe2+ chelating ability 36.4 ± 0.7%. In vitro cytotoxic tests suggested that LPPs had different effect on A549 cell line, in which corilagin (E) exhibited potent cytotoxicity with an IC50 value of 28.8 ± 1.2 μM. These findings were further confirmed by cell staining experiments. View Full-Text
Keywords: Longan pericarp; Shixia; polyphenol; HPLC; antioxidant; cytotoxicity Longan pericarp; Shixia; polyphenol; HPLC; antioxidant; cytotoxicity
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Bai, X.; Pan, R.; Li, M.; Li, X.; Zhang, H. HPLC Profile of Longan (cv. Shixia) Pericarp-Sourced Phenolics and Their Antioxidant and Cytotoxic Effects. Molecules 2019, 24, 619.

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