Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts
Abstract
:1. Introduction
2. Results and Discussion
2.1. Polyphenol Characterization by LC/UV/ESI-MS Analysis
2.2. Tea Extracts Yields, Polyphenol Content, Antioxidant Activity and Anti-Enzyme Activity
2.3. Antibacterial Activity
3. Materials and Methods
3.1. Starting Materials and Chemicals
3.2. Aqueous Extracts Preparation
3.3. Ethanol Extracts Preparation
3.4. LC-UV/ESI-MS Analysis
3.5. Ripe and Raw Components Characterization by High Resolution Mass Spectrometry
3.6. Determination of the Total Polyphenol Content
3.7. Determination of Total Flavonoid Content
3.8. Determination of Total Tannin Content
3.9. Determination of Free Amino Acid Content
3.10. Determination of Total Protein Content
3.11. Determination of Carbohydrate Content
3.12. Quantitative Determination of Caffeine
3.13. DPPH (2,2-Diphenyl-1-Picrylhydrazyl) Assay
3.14. Superoxide Anion Radical Scavenging Activity (SARSA)
3.15. ABTS Radical Cation Decolorization Assay
3.16. Determination of the Oxygen Radical Absorbing Capacity (ORAC)
3.17. FRAP Assay
3.18. Hyaluronidase Inhibition Activity
3.19. Tyrosinase Inhibition Activity
3.20. Elastase Inhibition Activity
3.21. Collagenase Inhibition Activity
3.22. Spectrophotometric Analyses
3.23. Antibacterial Activity
3.24. Principal Component Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Raw materials of ripe and raw pu-erh teas are available from the authors. |
Time | Water % | Acetonitrile % | Formic Acid % |
---|---|---|---|
0 | 5 | 0.1 | |
50 | 17 | 0.1 | |
80 | 32 | 0.1 | |
83 | 40 | 0.1 | |
95 | 5 | 0.1 |
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Roda, G.; Marinello, C.; Grassi, A.; Picozzi, C.; Aldini, G.; Carini, M.; Regazzoni, L. Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts. Molecules 2019, 24, 473. https://doi.org/10.3390/molecules24030473
Roda G, Marinello C, Grassi A, Picozzi C, Aldini G, Carini M, Regazzoni L. Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts. Molecules. 2019; 24(3):473. https://doi.org/10.3390/molecules24030473
Chicago/Turabian StyleRoda, Gabriella, Cristina Marinello, Anita Grassi, Claudia Picozzi, Giancarlo Aldini, Marina Carini, and Luca Regazzoni. 2019. "Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts" Molecules 24, no. 3: 473. https://doi.org/10.3390/molecules24030473